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  1. Long-term live imaging, cell identification and cell tracking in regenerating crustacean legs

    This article has 8 authors:
    1. Çağrı Çevrim
    2. Béryl Laplace-Builhé
    3. Ko Sugawara
    4. Maria Lorenza Rusciano
    5. Nicolas Labert
    6. Jacques Brocard
    7. Alba Almazán
    8. Michalis Averof
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This study presents a valuable technical advance in the long-term live imaging of limb regeneration at cellular resolution in Parhyale hawaiensis. The authors develop and carefully validate a method to continuously image entire regenerating legs over several days while minimizing photodamage and optimizing conditions for robust cell tracking, together with post-hoc in situ identification of cell types. The data are convincing, the methodology is rigorous and clearly documented, and the results will be of interest to researchers in regeneration biology, developmental biology, and advanced live imaging and cell tracking software development.

      [Editors' note: this paper was reviewed by Review Commons.]

    Reviewed by eLife, Review Commons

    This article has 8 evaluationsAppears in 2 listsLatest version Latest activity