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  1. Acquired resistance to sotorasib in KRASG12C mutant NSCLC is vulnerable to PI3K-mTOR pathway inhibition mediated by 4E-BP1 regulator of cap-dependent translation

    This article has 15 authors:
    1. Ismail M Meraz
    2. Shuhong Wu
    3. Yi Xu
    4. Lihui Gao
    5. Meng Feng
    6. Chenghui Ren
    7. Renduo Song
    8. Ran Zhang
    9. Qi Wang
    10. Yuanxin Xi
    11. Sung Yun Jung
    12. Jing Wang
    13. Bingliang Fang
    14. Mourad Majidi
    15. Jack A Roth
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      The authors investigate mechanisms of acquired resistance (AR) to KRAS-G12C inhibitors (sotorasib) in non-small cell lung cancer, proposing that resistance arises from signaling rewiring rather than additional mutations. While the study addresses a valuable clinical question, it is limited by several weaknesses in experimental rigor, data interpretation, and presentation, meaning the strength of evidence is incomplete

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    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  2. Systematic characterization of site-specific proline hydroxylation using hydrophilic interaction chromatography and mass spectrometry

    This article has 7 authors:
    1. Hao Jiang
    2. Jimena Druker
    3. James W Wilson
    4. Dalila Bensaddek
    5. Jason R Swedlow
    6. Sonia Rocha
    7. Angus I Lamond
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      The study presents a valuable resource of proline hydroxylation proteins for molecular biology studies in oxygen-sensing and cell signaling with the characterization of Repo-man proline hydroxylation site. The evidence supporting the claim of the authors is solid, although further clarification of the overall efficiency of the HILIC analysis, the specificity/sensitivity of immonium ion analysis, as well as quantification of proline hydroxylation identifications will be helpful. The work will be of interest to researchers studying post-translational modification, oxygen sensing, and cell signaling.

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    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  3. Acidocalcisome-like vacuoles constitute a feedback-controlled phosphate buffering system for the cytosol

    This article has 6 authors:
    1. Samuel Bru
    2. Lydie Michaillat Mayer
    3. Geun-Don Kim
    4. Danye Qiu
    5. Henning Jacob Jessen
    6. Andreas Mayer
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This study provides fundamental insights into eukaryotic phosphate homeostasis by demonstrating how yeast vacuoles dynamically regulate cytosolic phosphate levels. The conclusions are convincing, supported by an elegant combination of in vitro assays and in vivo measurements. This study will be of interest to cell biologists, particularly for those who are working in the field of phosphate metabolism.

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    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  4. Structural insights into the recruitment of viral Type 2 IRES to ribosomal preinitiation complex for protein synthesis

    This article has 2 authors:
    1. Deepakash Das
    2. Tanweer Hussain
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This manuscript offers valuable structural and mechanistic insights into the structure and assembly of the Type II internal ribosome entry site (IRES) from encephalomyocarditis virus (EMCV) and the translation initiation complex, revealing a direct interaction between the IRES and the 40S ribosomal subunit. While a solid cryo-EM method was used, enhancing the overall resolution or adding complementary biochemical data would further improve the clarity and impact of this study. This manuscript will attract researchers in cap-independent translation, host-pathogen interactions, and virology.

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    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  5. Task structure tailors the geometry of neural representations in human lateral prefrontal cortex

    This article has 4 authors:
    1. Apoorva Bhandari
    2. Haley Keglovits
    3. Defne Buyukyazgan
    4. David Badre
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This study presents a valuable finding on the representational structure of task encoding in the prefrontal cortex. The evidence supporting the claims of the authors is solid, representing an impressive data collection effort and best-practice fMRI analyses. However, at least including visual regions as a control and controlling for behavioral differences in the task in representation analyses would have strengthened the study. The work will be of interest to cognitive neuroscientists interested in the neural basis of cognitive control.

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    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  6. Dynamics of mesoscale brain network during decision-making learning revealed by chronic, large-scale single-unit recording

    This article has 5 authors:
    1. Tian-Yi Wang
    2. Chengcong Feng
    3. Chengyao Wang
    4. Chi Ren
    5. Zhengtuo Zhao
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This study presents experiments suggesting intriguing mesoscale reorganization of functional connectivity across distributed cortical and subcortical circuits during learning. The approach is technically impressive and the results are potentially of valuable significance. However, in its current form, the strength of evidence is incomplete. More in-depth analyses and the acquisition of data from additional animals in the primary experiment could bolster these findings.

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    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  7. Coordinated dynamics of excitatory and inhibitory synapse assembly

    This article has 3 authors:
    1. Krassimira Garbett
    2. James Allen
    3. Richard C Sando
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      In this valuable study, the authors developed long-term imaging tools to simultaneously monitor the temporal and spatial dynamics of excitatory and inhibitory synapses and reported that excitatory and inhibitory synapses need to develop synergistically during synaptogenesis to maintain balance. While the analysis and quantification of the imaging data are incomplete, there is convincing evidence that the developed tools are feasible. If these tools can function stably in vivo, their applications will be much broader.

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    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  8. Heterogenous associations of polygenic indices of 35 traits with mortality

    This article has 7 authors:
    1. Hannu Lahtinen
    2. Jaakko Kaprio
    3. Andrea Ganna
    4. Kaarina Korhonen
    5. Stefano Lombardi
    6. Karri Silventoinen
    7. Pekka Martikainen
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This valuable study reports convincing evidence about associations between 35 polygenic indices (PGIs) for social, behavioral, and psychological traits, along with some non-fatal health conditions (e.g., BMI) and all-cause mortality in data from Finnish population-based surveys and a twin cohort linked with administrative registers. PGIs for education, depression, alcohol use, smoking, BMI, and self-rated health showed the strongest associations with all-cause mortality, on the order of ~10% increment in risk per PGI standard deviation. Effect sizes from twin-difference analyses tended to be slightly larger than the effect sizes from population cohorts, opposite the pattern generally observed when testing PGI associations with their target phenotypes and supporting robustness of findings to confounding by population stratification.

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    This article has 3 evaluationsAppears in 1 listLatest version Latest activity
  9. Visual Working Memory Guides Attention Rhythmically

    This article has 3 authors:
    1. Jiachen Lu
    2. Yaochun Cai
    3. Xilin Zhang
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This valuable study reports evidence that items maintained in working memory can bias attention in an oscillatory manner, with the attentional capture effect fluctuating at theta frequency. The study provides incomplete evidence that this dynamic attentional bias is associated with oscillatory neural mechanisms, particularly in the alpha and theta bands, as measured by EEG. The study will be relevant for researchers studying attention, working memory, and neural oscillations, particularly those interested in how memory and perception interact over time.

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    This article has 3 evaluationsAppears in 1 listLatest version Latest activity
  10. A Context-Free Model of Savings in Motor Learning

    This article has 4 authors:
    1. Mahdiyar Shahbazi
    2. Olivier Codol
    3. Jonathan A Michaels
    4. Paul L Gribble
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This study presents valuable computational findings on the neural basis of learning new motor memories and the savings using recurrent neural networks. The evidence supporting the claims of the authors is solid, but it would benefit from more controls and from considering the role of explicit strategies and other brain regions. This work will be of interest to computational and experimental neuroscientists working in motor learning.

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    This article has 3 evaluationsAppears in 1 listLatest version Latest activity
  11. Feedback of peripheral saccade targets to early foveal cortex

    This article has 5 authors:
    1. Luca Kämmer
    2. Lisa M Kroell
    3. Tomas Knapen
    4. Martin Rolfs
    5. Martin N Hebart
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This useful study replicates a previous finding that information about peripherally presented visual stimuli is represented in the foveal visual cortex, and extends it by demonstrating that these representations are similar to those evoked by foveally presented stimuli. The authors' gaze-contingent fMRI design provides solid evidence for these findings. Some of the stronger theoretical claims, such as that the effects are due to predictive pre-saccadic remapping, are not fully supported by the current results.

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    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  12. Individual differences in fear memory expression engage distinct functional brain networks

    This article has 6 authors:
    1. Barbara D Fontana
    2. Jacob Hudock
    3. Neha Rajput
    4. Dea Kanini
    5. Dinh Luong
    6. Justin W Kenney
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This important work sets out to identify the neural substrates of associative fear responses in adult zebrafish. Through a compelling and innovative paradigm and analysis, the authors suggest brain regions associated with individual differences in fear memory. While several findings are well supported, aspects of the interpretation and presentation are partially incomplete, and the manuscript would benefit from adjusting key claims or including additional experiments. Nonetheless, this study showcases the strength of zebrafish for systems-level neuroscience and will be of broad interest to the neuroscience community.

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    This article has 8 evaluationsAppears in 1 listLatest version Latest activity
  13. Viral commitment to infection depends on host metabolism

    This article has 4 authors:
    1. Anastasios Marantos
    2. Kim Sneppen
    3. Stanley Brown
    4. Namiko Mitarai
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This valuable study shows the impact of the metabolic state of bacteria on phage infection. The experimental results, based on various phages infecting E. coli, are solid and consistent with a two-step adsorption mathematical model, although the detailed evidence supporting this model is currently incomplete. This study should be of interest to the communities working on cell metabolism and on host-pathogen interactions.

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    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  14. Cortical motor activity modulates respiration and reduces apnoea in neonates

    This article has 8 authors:
    1. Coen S Zandvoort
    2. Fatima Usman
    3. Shellie Robinson
    4. Odunayo Fatunla
    5. Eleri Adams
    6. Kyle TS Pattinson
    7. Simon F Farmer
    8. Caroline Hartley
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      Zandvoort and colleagues describe respiration-brain coupling in the context of apnoea in human newborns. The authors have addressed an important question and supported their claims with solid data. The rigor of the findings could perhaps be further strengthened with some relatively minor changes to the analysis methodology.

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    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  15. Beta-Glucan Modulates Monocyte Plasticity and Differentiation Capacity to Mitigate DSS-Induced Colitis

    This article has 16 authors:
    1. Yinyin Lv
    2. Yanyun Fan
    3. Qingxiang Gao
    4. Qiongyun Chen
    5. Yiqun Hu
    6. Lin Wang
    7. Huaxiu Shi
    8. Ermei Chen
    9. Qinyu Xu
    10. Ying Cai
    11. Qingqi Fan
    12. Linying Li
    13. Dan Du
    14. Jianlin Ren
    15. Shih-Chin Cheng
    16. Hongzhi Xu
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This study presents valuable and compelling evidence that β-glucan-induced trained immunity can protect against intestinal inflammation by reprogramming innate immune cells toward a reparative phenotype. The authors employ a convincing combination of functional assays, adoptive transfers, and single-cell transcriptomics to uncover mechanistic insights and demonstrate the therapeutic potential of innate immune memory in IBD. While the work is robust, addressing the underlying epigenetic mechanisms and including additional controls would further reinforce the trained immunity-specific interpretation.

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    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  16. Synthetic auxotrophy reveals metabolic regulation of plasma cell generation, affinity maturation, and cytokine receptor signaling

    This article has 16 authors:
    1. Sung Hoon Cho
    2. Shawna K Brookens
    3. Ariel L Raybuck
    4. Kaylor Meyer
    5. Yeeun C Paik
    6. Jennie Hamilton
    7. Jingxin Li
    8. Karel Kalecky
    9. Chloe Park
    10. Sakeenah L Hicks
    11. John Karijolich
    12. Teodoro H Bottiglieri
    13. Jeffrey C Rathmell
    14. Denis Mogilenko
    15. Chris D Scharer
    16. Mark R Boothby
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      The authors addressed an important biological question, namely the role of glutamine metabolism in humoral responses, and they obtained solid conclusions. The strength of this study is that the authors used state-of-the-art transgenic mouse models together with in vitro analysis, thereby providing significant insights into the question posed. The following would strengthen the manuscript: i) adding more in-depth functionality/physiological relevance in the discussion part, and ii) regarding the experiments, the inclusion of more appropriate controls and a clearer and more accurate description of the methods.

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    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  17. PHD1-dependent hydroxylation of RepoMan (CDCA2) on P604 modulates the control of mitotic progression

    This article has 12 authors:
    1. Jimena Druker
    2. Hao Jiang
    3. Dilem Shakir
    4. Fraser Child
    5. Vanesa Alvarez
    6. Melpomeni Platani
    7. Andrea Corno
    8. Constance Alabert
    9. Adrian T Saurin
    10. Jason R Swedlow
    11. Sonia Rocha
    12. Angus I Lamond
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This compelling work describes how the cell cycle-regulating phosphatase subunit, RepoMan, is regulated by the oxygen-dependent, metabolite-sensing hydroxylase PHD1. The characterisation of how proline hydroxylation alters signalling at the molecular and cellular level provides important evidence to enhance our understanding of how 2-oxoglutarate-dependent dioxygenases influence the cell cycle and mitosis.

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    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  18. SLC4A1 mutations that cause distal renal tubular acidosis alter cytoplasmic pH and cellular autophagy

    This article has 12 authors:
    1. Grace Essuman
    2. Midhat Rizvi
    3. Ensaf Almomani
    4. Shahid AK M Ullah
    5. Sarder MA Hasib
    6. Forough Chelangarimiyandoab
    7. Priyanka Mungara
    8. Manfred J Schmitt
    9. Marguerite Hureaux
    10. Rosa Vargas-Poussou
    11. Nicolas Touret
    12. Emmanuelle Cordat
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This work characterizes the function and localization of SLC4A1 variants associated with distal renal tubular acidosis in human patients. Cell culture and limited animal studies provide partial but incomplete support to the authors' claim that the variants disrupt normal protein degradative flux by alkalinizing the intracellular pH. The study is valuable in providing preliminary evidence for future exploration of the link between intracellular pH regulation by SLC4A1 and kidney cell function in vivo.

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    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  19. Optimised genome editing for precise DNA insertion and substitution using Prime Editors in zebrafish

    This article has 11 authors:
    1. Yosuke Ono
    2. Martin Peterka
    3. Michael Love
    4. Ashish Bhandari
    5. Euan Gordon
    6. Jonathan S Ball
    7. Charles R Tyler
    8. Steve Rees
    9. Mohammad Bohlooly-Y
    10. Marcello Maresca
    11. Steffen Scholpp
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This study presents a valuable comparison of the efficiency and precision of two prime editing methods to introduce single-nucleotide variants and longer exogenous DNA sequences into the zebrafish genome. Solid data support the conclusion that the PE2 prime editor Nickase is more effective at introducing single-nucleotide variants, while the PEn prime editor nuclease is more effective at integrating short sequences from 3 up to 30 base pairs, for both somatic and germline editing. The results will be of interest to the zebrafish community, in particular to model human disease variants in this model organism.

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    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  20. Decoding movie content from neuronal population activity in the human medial temporal lobe

    This article has 12 authors:
    1. Franziska Gerken
    2. Alana Darcher
    3. Pedro J Gonçalves
    4. Rachel Rapp
    5. Ismail Elezi
    6. Johannes Niediek
    7. Marcel S Kehl
    8. Thomas P Reber
    9. Stefanie Liebe
    10. Jakob H Macke
    11. Florian Mormann
    12. Laura Leal-Taixé
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This valuable study demonstrates that it is possible to decode information about characters and locations from single-unit responses in the human brain to a narrative movie, using a convincing technical approach to capture information in population-level dynamics. The study introduces an exciting dataset of single-unit responses in humans during a naturalistic and dynamic movie stimulus, with recordings from multiple regions within the medial temporal lobe. Using both a traditional firing-rate analysis as well as a population decoding analysis to connect these neural responses to the visual content of the movie, they show that in this dataset, the decoding of semantic scene features (e.g., the person currently on screen), but not scene transitions, is surprisingly driven by classically non-responsive neurons. Based on these findings, the authors argue that dynamic naturalistic semantic information may be processed within the medial temporal lobe at the population level.

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    This article has 5 evaluationsAppears in 1 listLatest version Latest activity