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  1. Impaired Myofibroblast Proliferation is a Central Feature of Pathologic Post-Natal Alveolar Simplification

    This article has 8 authors:
    1. Imran S. Khan
    2. Christopher Molina
    3. Xin Ren
    4. Vincent C. Auyeung
    5. Max Cohen
    6. Tatsuya Tsukui
    7. Amha Atakilit
    8. Dean Sheppard
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study unveils important mechanistic insights into postnatal lung development and bronchopulmonary dysplasia (BPD) pathology. Using two BPD models enhances our comprehension of the disease, utilizing compelling evidence from single-cell sequencing and flow cytometry, revealing a myofibroblast loss. Pharmacological and genetic approaches convincingly argue against the presumed increase in TGFb signaling causing alveolar simplification; instead, it appears to be a compensatory response. The identified weakness is the absence of validation in tissue, leaving the question unanswered regarding whether myofibroblast loss is due to a lack of myofibroblast proliferation or myofibroblast differentiation/specification.

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    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  2. Diverging roles of TRPV1 and TRPM2 in warm-temperature detection

    This article has 4 authors:
    1. Muad Y. Abd El Hay
    2. Gretel B. Kamm
    3. Alejandro Tlaie
    4. Jan Siemens
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      In this important manuscript, Abd El Hay and colleagues reveal a clear role of TRPV1 and TRPM2 receptors in warm temperature perception and present a technically unique experimental strategy to measure and analyze temperature preference behavior, which will have a lasting impact on the field. In addition to the behavioral data, which is strong, the study provides an analysis of cultured sensory neurons to controlled warmth stimuli - in this case, the evidence relating the activity of TRPM2 channels to the behavioral responses of animals is incomplete. Overall, the findings are of importance for neuroscientists, physiologists, and biophysicists, as there is still substantial discussion in the field regarding the contribution of TRP channels to different aspects of thermosensation.

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    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  3. The Lateral Ganglionic Eminence Does Not Generate Cortical Oligodendrocytes

    This article has 10 authors:
    1. Jialin Li
    2. Feihong Yang
    3. Yu Tian
    4. Ziwu Wang
    5. Dashi Qi
    6. Zhengang Yang
    7. Jiangang Song
    8. Jing Ding
    9. Xin Wang
    10. Zhuangzhi Zhang
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The authors provide solid evidence that any contribution of oligodendrocyte precursors to the developing cortex from the lateral ganglionic eminence is minimal in scope. The methods used support the conclusions, with some technical concerns that the authors can address with further experimentation. These are considered valuable additions to our understanding of the origins of oligodendrocytes in the forebrain during development.

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    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  4. Neutralizing gut-derived lipopolysaccharide as a novel therapeutic strategy for severe leptospirosis

    This article has 6 authors:
    1. Xufeng Xie
    2. Xi Chen
    3. Shilei Zhang
    4. Jiuxi Liu
    5. Wenlong Zhang
    6. Yongguo Cao
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      Herein, Xie and colleagues use a hamster model to show that Leptospira infection leads to gut pathology, an altered gut microbiota, and increased translocation. A combined use of antibiotics and LPS neutralization prolonged survival, providing a potential new therapeutic approach. This fundamental study uses compelling methods to provide new insights into this emerging disease, which could be dissected further in future studies aimed at gaining mechanistic insight and assessing the translational relevance of these discoveries.

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    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  5. Distinct T Cell Receptor (TCR) gene segment usage and MHC-restriction between foetal and adult thymus

    This article has 6 authors:
    1. Jasmine Rowell
    2. Ching-In Lau
    3. Susan Ross
    4. Diana C. Yanez
    5. Benny Chain
    6. Tessa Crompton
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This manuscript provides potentially valuable information suggesting that the earliest appearing T-cells during ontogeny may have properties that are fundamentally distinct from those appearing later in life. At this stage, weaknesses in the experimental design and data interpretation provide inadequate support for the conclusions. With modifications, the paper should be of interest to those interested in T-cell development.

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    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  6. Convergent Epigenetic Evolution Drives Relapse in Acute Myeloid Leukemia

    This article has 8 authors:
    1. Kevin A. Nuno
    2. Armon Azizi
    3. Thomas Köhnke
    4. Caleb A. Lareau
    5. Asiri Ediwirickrema
    6. M. Ryan Corces
    7. Ansuman T. Satpathy
    8. Ravindra Majeti
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important work substantially advances our understanding of how resistant leukemia can arise without changes in mutational patterns by displaying epigenetic changes. The evidence supporting the conclusions is compelling, with rigorous genomic assays done on primary samples. and state-of-the-art microscopy. The work will be of broad interest to hematologists and cancer biologists.

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    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  7. Olfactory learning in Drosophila requires O-GlcNAcylation of mushroom body ribosomal subunits

    This article has 13 authors:
    1. Haibin Yu
    2. Dandan Liu
    3. Yaowen Zhang
    4. Ruijun Tang
    5. Xunan Fan
    6. Song Mao
    7. Lu Lv
    8. Fang Chen
    9. Hongtao Qin
    10. Zhuohua Zhang
    11. Daan M.F. van Aalten
    12. Bing Yang
    13. Kai Yuan
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This work describes a valuable new technique involving proximity labelling to identify Drosophila proteins modified by GlcNAcylation in subsets of cells in vivo. A solid set of experiments shows that several ribosomal proteins are modified in the fly mushroom body. Consistent with a role for GlcNAcylation of ribosomal proteins in control of memory related translational control, the authors show that perturbation of GlcNAc modification in KCs prevents efficient consolidation of long-term memory.

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    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  8. A Novel Mouse Model for LAMA2 -Related Muscular Dystrophy: Analysis of Molecular Pathogenesis and Clinical Phenotype

    This article has 10 authors:
    1. Dandan Tan
    2. Yidan Liu
    3. Huaxia Luo
    4. Qiang Shen
    5. Xingbo Long
    6. Luzheng Xu
    7. Jieyu Liu
    8. Nanbert Zhong
    9. Hong Zhang
    10. Hui Xiong
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This useful manuscript reports on a new mouse model for LAMA2-MD, a rare but very severe congenital muscular dystrophy; the knockout mice were generated by removing exon3 in the Lama2 gene, which results in a frameshift in exon4 and a premature stop codon. These animals lack any laminin-alpha2 protein and confirm results from previous Lama2 knockout models. Additionally, this study includes transcriptomics data that might be a good resource for the field. However, the experimental evidence supporting the main claims of the manuscript is incomplete, citations of previous Lama2 null mice studies are lacking, and both data presentation and interpretation need improvement.

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    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  9. Excitatory neurons in stratum radiatum provide an alternative pathway for excitation flow that escapes perisomatic inhibition

    This article has 10 authors:
    1. Julia Lebedeva
    2. David Jappy
    3. Azat Nasretdinov
    4. Alina Vazetdinova
    5. Viktoria Krut’
    6. Rostislav Sokolov
    7. Yulia Dobryakova
    8. Marina Eliava
    9. Valery Grinevich
    10. Andrei Rozov
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This work addresses the connectivity of giant excitatory neurons in a part of CA1 of the hippocampus. Recordings in rat brain slices provide new evidence that these cells excite bistratified and basket inhibitory neurons, and have weak inhibitory input from basket cells, as well as other findings. This circuitry gives these cells unique potential, making the work valuable, however the strength of the evidence is currently incomplete.

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    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  10. Secreted antigen A peptidoglycan hydrolase is essential for Enterococcus faecium cell separation and priming of immune checkpoint inhibitor cancer therapy

    This article has 9 authors:
    1. Steven Klupt
    2. Kyong Tkhe Fam
    3. Xing Zhang
    4. Pavan Kumar Chodisetti
    5. Abeera Mehmood
    6. Tumara Boyd
    7. Danielle Grotjahn
    8. Donghyun Park
    9. Howard C. Hang
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      The authors build upon prior data implicating the secreted peptidoglycan hydrolase SagA produced by Enterococcus faecium in immunotherapy. Leveraging new strains with sagA deletion/complementation constructs, the investigators reveal that sagA is non-essential, with sagA deletion leading to a marked growth defect due to impaired cell division, and sagA being necessary for the immunogenic and anti-tumor effects of E. faecium. In aggregate, the study utilizes compelling methods to provide both fundamental new insights into E. faecium biology and host interactions and a proof-of-concept for identifying the bacterial effectors of immunotherapy response.

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    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  11. The genetic architecture of the load linked to dominant and recessive self-incompatibility alleles in Arabidopsis halleri and A. lyrata

    This article has 8 authors:
    1. Le Veve Audrey
    2. Genete Mathieu
    3. Lepers-Blassiau Christelle
    4. Ponitzki Chloé
    5. Céline Poux
    6. Vekemans Xavier
    7. Durand Eleonore
    8. Castric Vincent
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study presents valuable empirical work and simulations that are relevant for the evolution of genetic load linked to self-incompatibility alleles in Arabidopsis. The evidence supporting the findings is solid but could be improved by a more detailed quantitative assessment of the impacts of deleterious alleles and their dominance. The simulation results are somewhat incomplete, as details of the approach and code do not appear to be available, but this could be easily remedied. The work will be of relevance to geneticists interested in the evolution of allelic diversity in similar systems.

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    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  12. Glial ferritin maintains neural stem cells via transporting iron required for self-renewal in Drosophila

    This article has 5 authors:
    1. Zhixin Ma
    2. Wenshu Wang
    3. Xiaojing Yang
    4. Menglong Rui
    5. Su Wang
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study, which seeks to identify factors from the glial niche that support and maintain neural stem cells, unveils a novel role for ferritin in this process. Furthermore, the work shows that defects in larval brain development resulting from ferritin knockdown can be attributed to impaired Fe-S cluster activity and ATP production. These findings will be valuable to both oncologists and neurobiologists, though the supporting evidence is currently incomplete.

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    This article has 5 evaluationsAppears in 2 listsLatest version Latest activity
  13. A Statistical Framework for Analysis of Trial-Level Temporal Dynamics in Fiber Photometry Experiments

    This article has 4 authors:
    1. Gabriel Loewinger
    2. Erjia Cui
    3. David Lovinger
    4. Francisco Pereira
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This important work presents a new methodology for the statistical analysis of fiber photometry data, improving statistical power while avoiding the bias inherent in the choices that are necessarily made when summarizing photometry data. The reanalysis of two recent photometry data sets, the simulations, and the mathematical detail provide convincing evidence for the utility of the method and the main conclusions, however, the discussion of the re-analyzed data is incomplete and would be improved by a deeper consideration of the limitations of the original data. In addition, consideration of other data sets and photometry methodologies including non-linear analysis tools, as well as a discussion of the importance of the data normalization are needed.

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    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  14. Intrinsic dynamics of randomly clustered networks generate place fields and preplay of novel environments

    This article has 5 authors:
    1. Jordan Breffle
    2. Hannah Germaine
    3. Justin D. Shin
    4. Shantanu P. Jadhav
    5. Paul Miller
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study presents a valuable finding on the spontaneous emergence of structured activity in artificial neural networks endowed with specific connectivity profiles. The evidence supporting the claims of the authors is potentially solid but still incomplete at this stage, as it is important to demonstrate that similar properties are observed with more diverse inputs and in more complex environments. The work will be of interest to systems and computational neuroscientists.

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    This article has 5 evaluationsAppears in 2 listsLatest version Latest activity
  15. PKA regulation of neuronal function requires the dissociation of catalytic subunits from regulatory subunits

    This article has 3 authors:
    1. Wei-Hong Xiong
    2. Maozhen Qin
    3. Haining Zhong
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      In this important study, Xiong and colleagues studied PKA regulation in synaptic plasticity. They provide convincing evidence that dissociation of PKA catalytic subunits is essential for the proper function of the kinase. Experiments using a PKA regulatory-catalytic subunit fusion establish that dissociative activation is required for both structural long-term potentiation and basal priming of AMPA receptors.

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    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  16. Heron: A Knowledge Graph editor for intuitive implementation of python based experimental pipelines

    This article has 4 authors:
    1. George Dimitriadis
    2. Ella Svahn
    3. Andrew MacAskill
    4. Athena Akrami
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This manuscript presents a valuable, lightweight software package that aims to accelerate the implementation of experiment pipelines running on heterogeneous computer environments. The authors present solid evidence of the advantages of their chosen approach, including demonstrating the flexibility, ease of use, and practical utility of this new system. However, questions remain regarding the maturity of the project and its specific advantages in relation to existing widely adopted software packages.

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    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  17. Binding of PUFA stabilizes a conductive state of the selectivity filter in IKs channels

    This article has 9 authors:
    1. Alessia Golluscio
    2. Jodene Eldstrom
    3. Jessica J. Jowais
    4. Marta E. Perez-Rodriguez
    5. Kevin P. Cunningham
    6. Alicia de la Cruz
    7. Xiaoan Wu
    8. David Fedida
    9. H. Peter Larsson
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This study makes a valuable finding, a polyunsaturated fatty acid increases the conductance of a K+ channel by helping its K+ selectivity filter form a conductive state. Overall, support for this major claim is solid, though other claims remain speculative with incomplete support. These findings are expected to be of interest to researchers studying ion channel gating.

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    This article has 4 evaluationsAppears in 3 listsLatest version Latest activity
  18. Senescent cells inhibit muscle differentiation via the lipid- SASP 15d-PGJ2 mediated modification and control of HRas

    This article has 7 authors:
    1. Swarang Sachin Pundlik
    2. Snehasudha Subhadarshini Sahoo
    3. Alok Barik
    4. Ashwin Venkateshvaran
    5. Mahapatra Anshuman Jaysingh
    6. Raviswamy G H Math
    7. Arvind Ramanathan
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This manuscript provides useful information about the lipid metabolite 15d-PGJ2 as a potential regulator of myoblast senescence. The authors provide experimental evidence that 15d-PGJ2 inhibits myoblast proliferation and differentiation by binding and regulating HRas. However, the manuscript is incomplete in its current form, as it lacks robust support from the data regarding the main conclusions related to senescence and technical concerns related to the senescence models used in this study.

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    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  19. Activation of Polycystin-1 Signaling by Binding of Stalk-derived Peptide Agonists

    This article has 6 authors:
    1. Shristi Pawnikar
    2. Brenda S. Magenheimer
    3. Ericka Nevarez Munoz
    4. Allan Haldane
    5. Robin L. Maser
    6. Yinglong Miao
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      This joint computational/experimental study demonstrates the ability of synthetic peptides derived from the stalk-tethered agonist in Polycystin-1 (PC1) to re-activate signaling by a stalkless C-terminal fragment of PC1. The study is valuable as it discovered peptide agonists for PC1 and the integrated in vitro and in silico approach is potentially applicable to the analysis of related systems. The line of evidence presented in the current manuscript is considered incomplete and additional experiments are needed as controls and for validating the simulations.

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    This article has 4 evaluationsAppears in 2 listsLatest version Latest activity
  20. SntB triggers the antioxidant pathways to regulate development and aflatoxin biosynthesis in Aspergillus flavus

    This article has 12 authors:
    1. Dandan Wu
    2. Chi Yang
    3. Yanfang Yao
    4. Dongmei Ma
    5. Hong Lin
    6. Ling Hao
    7. Wenwen Xin
    8. Kangfu Ye
    9. Minghui Sun
    10. Yule Hu
    11. Yanling Yang
    12. Zhenhong Zhuang
    This article has been curated by 1 group:
    • Curated by eLife

      eLife assessment

      In this useful study, the authors investigate the regulatory mechanisms related to toxin production and pathogenicity in Aspergillus flavus. Their observations indicate that the SntB protein regulates morphogenesis, aflatoxin biosynthesis, and the oxidative stress response, however, the data supporting these conclusions are incomplete. The work will be of interest to bacteriologists.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity