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  1. Unreliable homeostatic action potential broadening in cultured dissociated neurons

    This article has 16 authors:
    1. Andreas Ritzau-Jost
    2. Salil Rajayer
    3. Jana Nerlich
    4. Filip Maciag
    5. Alexandra John
    6. Michael Russier
    7. Victoria Gonzalez Sabater
    8. Luke J. Steiger
    9. Jacques-Olivier Coq
    10. Jens Eilers
    11. Maren Engelhardt
    12. Juan Burrone
    13. Dominique Debanne
    14. Martin Heine
    15. Stephen M Smith
    16. Stefan Hallermann
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This study provides compelling evidence that action potential (AP) broadening is not a universal feature of homeostatic plasticity in response to chronic activity deprivation. By leveraging state-of-the-art methods across multiple brain regions and laboratories, the authors demonstrate that AP half-width remains largely stable, challenging previous assumptions in the field. These important findings help resolve longstanding inconsistencies in the literature and significantly advance our understanding of neuronal network homeostasis.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  2. Glycosylated IgG antibodies accelerated the recovery of haemorrhagic fever with renal syndrome patients

    This article has 15 authors:
    1. Chuansong Quan
    2. Lu Wang
    3. Jiming Gao
    4. Yaoni Li
    5. Xiaoyu Xu
    6. Houqiang Li
    7. Zixuan Gao
    8. Wenxu Ruan
    9. Hongzhi Liu
    10. Qian Li
    11. Weijia Xing
    12. Liqiong Zhao
    13. Michael J. Carr
    14. Weifeng Shi
    15. Haifeng Hou
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      The authors investigated the potential role of IgG N-glycosylation in Haemorrhagic Fever with Renal Syndrome (HFRS), which may offer significant insights for understanding molecular mechanisms and for the development of therapeutic strategies for this infectious disease. The findings are useful to the field, although the strength of evidence to support the findings is incomplete. Several issues need to be addressed, including more detail on the background, methods, and results. Additional statistical tests should be performed, and the conclusions should reflect the correlational findings of the paper.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 1 listLatest version Latest activity
  3. C. elegans food choice exhibits effort discounting-like behavior

    This article has 6 authors:
    1. Jonathan R. M. Millet
    2. Serge Faumont
    3. Aaron B. Schatz
    4. Amanda M. White
    5. Kathy D. Chicas-Cruz
    6. Shawn R. Lockery
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This important work develops C. elegans as a model organism for studying effort-based discounting by asking the worms to choose between easy and hard to digest bacteria. The authors provide convincing evidence that the nematodes are effort-discounting. However, evidence regarding the role of dopamine is incomplete and this weakens the authors connection of the behavior in C. elegans with mammals.

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    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  4. The Self-Interest of Adolescents Overrules Cooperation in Social Dilemmas

    This article has 6 authors:
    1. Xiaoyan Wu
    2. Hongyu Fu
    3. Gökhan Aydogan
    4. Chunliang Feng
    5. Shaozheng Qin
    6. Chao Liu
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This valuable work investigates cooperative behaviors in adolescents using a repeated Prisoner's Dilemma game. The computational modeling approach used in the study is solid and well established, yet evidence supporting certain claims remains incomplete. The work could be strengthened with the consideration of additional experimental contexts, non-linear relationships between age and observed behavior, and modeling details. If these concerns are addressed, the results will be of interest to developmental psychologists, economists, and social psychologists.

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    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  5. Inducible, virus-free direct lineage reprogramming enhances scalable generation of human inner ear hair cell-like cells

    This article has 11 authors:
    1. Robert N. Rainey
    2. Sam D. Houman
    3. Louise Menendez
    4. Ryan Chang
    5. Litao Tao
    6. Helena Bugacov
    7. Andrew P. McMahon
    8. Radha Kalluri
    9. John S. Oghalai
    10. Andrew K. Groves
    11. Neil Segil
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      The findings of this important study substantially advance our understanding of the transcription factors that can induce hair cell-like cells from human pluripotent stem cells. The presented evidence supporting these findings is compelling, including rigorous characterization of the effects of hair cell induction using both single-cell RNA sequencing and electrophysiological assessments.

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    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  6. Single-cell profiling of trabecular meshwork identifies mitochondrial dysfunction in a glaucoma model that is protected by vitamin B3 treatment

    This article has 12 authors:
    1. Nicholas Tolman
    2. Taibo Li
    3. Revathi Balasubramanian
    4. Guorong Li
    5. Violet Bupp-Chickering
    6. Ruth A. Kelly
    7. Marina Simón
    8. John Peregrin
    9. Christa Montgomery
    10. W. Daniel Stamer
    11. Jiang Qian
    12. Simon W.M. John
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This is a fundamental study that provides a detailed single-cell transcriptomic and epigenomic map of the mouse trabecular meshwor, identifying three distinct trabecular meshwor subtypes with specific functional roles. It links the glaucoma-associated transcription factor LMX1B to mitochondrial regulation in TM3 cells and demonstrates that nicotinamide treatment prevents IOP elevation in Lmx1bV265D/+ mutant mice, highlighting a potential metabolic therapeutic strategy for glaucoma. This convincing work would be further supported by data that link the transcriptional data with mitochondrial functional assays.

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    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  7. Passive muscle forces in Drosophila are large but insufficient to support a fly’s weight

    This article has 7 authors:
    1. Ninghan Wang
    2. Helene Babski
    3. Jonathan Elliot Perdomo
    4. Sarah Beth McMahan
    5. Arun Ramakrishnan
    6. Tirthabir Biswas
    7. Vikas Bhandawat
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This valuable study introduces a novel experimental and modeling framework to quantify passive joint torques in Drosophila, revealing that passive forces are insufficient to support body weight, contrary to prior assumptions based on larger insects. The approach is technically impressive, combining genetic silencing, kinematic tracking, and biomechanical modeling. However, the strength of evidence is incomplete, limited by concerns about the specificity of the genetic tools, simplifications in the mechanical model, and limited functional interpretation.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  8. Longitudinal tracking of neuronal activity from the same cells in the developing brain using Track2p

    This article has 7 authors:
    1. Jure Majnik
    2. Manon Mantez
    3. Sofia Zangila
    4. Stéphane Bugeon
    5. Leo Guignard
    6. Jean-Claude Platel
    7. Rosa Cossart
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This important study presents a new method for longitudinally tracking cells in two-photon imaging data that addresses the specific challenges of imaging neurons in the developing cortex. It provides compelling evidence demonstrating reliable longitudinal identification of neurons across the second postnatal week in mice. The study should be of interest to development neuroscientists engaged in population-level recordings using two-photon imaging.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  9. A quantitative pipeline for whole-mount deep imaging and analysis of multi-layered organoids across scales

    This article has 8 authors:
    1. Alice Gros
    2. Jules Vanaret
    3. Valentin Dunsing-Eichenauer
    4. Agathe Rostan
    5. Philippe Roudot
    6. Pierre-Francois Lenne
    7. Leo Guignard
    8. Sham Tlili
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This important study introduces a powerful imaging approach that enables deep-tissue visualization in gastruloids using two-photon microscopy, combined with spectral imaging and unmixing to achieve four-color 3D image acquisition. The evidence is compelling that many of the established methods are very helpful (e.g., registration, corrections, signal normalisation, lazy loading bioimage visualisation, spectral decomposition analysis), facilitate the development of quantitative research, and would be of interest to the wider scientific community.

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    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  10. SETD2 suppresses tumorigenesis in a KRASG12C-driven lung cancer model and its catalytic activity is regulated by histone acetylation

    This article has 11 authors:
    1. Ricardo J Mack
    2. Natasha M Flores
    3. Geoffrey C Fox
    4. Hanyang Dong
    5. Metehan Cebeci
    6. Simone Hausmann
    7. Tourkian Chasan
    8. Jill M Dowen
    9. Brian D Strahl
    10. Pawel K Mazur
    11. Or Gozani
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This is an important study providing molecular insight into how cross-talk between histone modifications regulates the histone H3K36 methyltransferase SETD2. The manuscript contains excellent quality data, and the conclusions are convincing and justified. This work will be of interest to many biochemists working in the field of chromatin biology and epigenetics.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  11. Mechanism of SK2 channel gating and its modulation by the bee toxin apamin and small molecules

    This article has 10 authors:
    1. Samantha J Cassell
    2. Weiyan Li
    3. Simon Krautwald
    4. Maryam Khoshouei
    5. Yan Tony Lee
    6. Joyce Hou
    7. Wendy Guan
    8. Stefan Peukert
    9. Wilhelm A Weihofen
    10. Jonathan R Whicher
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      In this important manuscript, Cassell and colleagues set out on a mechanistic and pharmacological exploration of an engineered chimeric small conductance calcium-activated potassium channel 2 (SK2). They show convincing evidence that the SK2 channel possesses a unique extracellular structure that modulates the conductivity of the selectivity filter, and that this structure is the target for the SK2 inhibitor apamin. While the interpretations are sound and the writing is clear, the manuscript would be strengthened by providing more detailed information for the electrophysiological experiments and the structural analyses attempted, in addition to relating dilation of the filter to mechanisms of inactivation in other potassium channels. This high-quality study will be of interest to membrane protein structural biologists, ion channel biophysicists, and chemical biologists, and will inform future drug development targeting SK channels.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  12. Multiple pathways prevent bi-parental mitochondria transmission in C. elegans

    This article has 5 authors:
    1. Valentine Melin
    2. Justine Cailloce
    3. Fanny Husson
    4. Jorge Merlet
    5. Vincent Galy
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This valuable work used molecular biology, cell biology, and genetic approaches to unravel individual genes and potential pathways that contribute to paternal mitochondrial inheritance using C. elegans as the model organism. Their microscopy method is cutting edge, with sufficient biological replicates, proper control, and appropriate statistics. These findings are convincing and are of general interest for understanding mitochondrial inheritance in C. elegans, which could have implications for understanding similar biological processes in other organisms.

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    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  13. A high-resolution analysis of arrestin2 interactions responsible for CCR5 endocytosis

    This article has 6 authors:
    1. Ivana Petrovic
    2. Samit Desai
    3. Polina Isaikina
    4. Layara Akemi Abiko
    5. Anne Spang
    6. Stephan Grzesiek
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      The authors investigate arrestin2-mediated CCR5 endocytosis in the context of clathrin and AP2 contributions. Using an extensive set of NMR experiments, and supported by microscopy and other biophysical assays, the authors provide convincing data on the roles of AP2 and clathrin in CCR5 endocytosis. This important work will appeal to an audience beyond those studying chemokine receptors, including those studying GPCR regulation and trafficking. The distinct role of AP2 and not clathrin will be of particular interest to those studying GPCR internalization mechanisms.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  14. Long-term live imaging, cell identification and cell tracking in regenerating crustacean legs

    This article has 8 authors:
    1. Çağrı Çevrim
    2. Béryl Laplace-Builhé
    3. Ko Sugawara
    4. Maria Lorenza Rusciano
    5. Nicolas Labert
    6. Jacques Brocard
    7. Alba Almazán
    8. Michalis Averof
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This study presents a valuable technical advance in the long-term live imaging of limb regeneration at cellular resolution in Parhyale hawaiensis. The authors develop and carefully validate a method to continuously image entire regenerating legs over several days while minimizing photodamage and optimizing conditions for robust cell tracking, together with post-hoc in situ identification of cell types. The data are convincing, the methodology is rigorous and clearly documented, and the results will be of interest to researchers in regeneration biology, developmental biology, and advanced live imaging and cell tracking software development.

      [Editors' note: this paper was reviewed by Review Commons.]

    Reviewed by eLife, Review Commons

    This article has 8 evaluationsAppears in 2 listsLatest version Latest activity
  15. Xcr1+ type 1 conventional dendritic cells are essential mediators for atherosclerosis progression

    This article has 17 authors:
    1. Tianhan Li
    2. Liaoxun Lu
    3. Juanjuan Qiu
    4. Xin Dong
    5. Le Yang
    6. Kexin He
    7. Yanrong Gu
    8. Binhui Zhou
    9. Tingting Jia
    10. Toby Lawrence
    11. Marie Malissen
    12. Guixue Wang
    13. Rong Huang
    14. Hui Wang
    15. Bernard Malissen
    16. Yinming Liang
    17. Lichen Zhang
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This manuscript by Li, Lu et al., presents important findings on the role of cDC1 in atherosclerosis and their influence on the adaptive immune system. Using Xcr1Cre-Gfp Rosa26LSL-DTA ApoE-/- mouse models, these data convincingly reveal an unexpected, non-redundant role of the XCL1-XCR1 axis in mediating cDC1 contributions to atherosclerosis.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 1 listLatest version Latest activity
  16. An abundant merozoite surface protein of Plasmodium falciparum modulates susceptibility to inhibitory antibodies

    This article has 11 authors:
    1. Isabelle G Henshall
    2. Jill Chmielewski
    3. Dimuthu Angage
    4. Ornella Romeo
    5. Keng Heng Lai
    6. Kaitlin R Turland
    7. Nicki Badii
    8. Michael Foley
    9. Robin F Anders
    10. James Beeson
    11. Danny W Wilson
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This important work offers a fresh perspective central to merozoite surface biology and potential implications on vaccine design, challenging the dogma that MSPs are indispensable invasion engines. Although the authors only deleted bp 132-819, the data based on Western blot, IFA, and RNA‐seq provide compelling evidence that while MSP2 is dispensable for growth, it serves as an immune modulator for AMA1. This work will be of particular interest to scientists working on different aspects of Plasmodium biology and vaccinology.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 1 listLatest version Latest activity
  17. REPOP: bacterial population quantification from plate counts

    This article has 6 authors:
    1. Pedro Pessoa
    2. Carol Lu
    3. Stanimir Asenov Tashev
    4. Rory Kruithoff
    5. Douglas P Shepherd
    6. Steve Pressé
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This important study introduces a Bayesian method to determine bacterial counts that accounts for the experimental noise inherent to dilution and plating methods, and distinguishes it from biological uncertainty. The evidence supporting the conclusions is convincing, combining simulated data and experimental data. The method will be of interest to microbial ecologists, and potentially to the broader community interested in inference from biological data, even more so if the domain of application and the limitations are further clarified.

    Reviewed by eLife

    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  18. Noninvasive ultrasound targeted modulation of calcium influx in splenic immunocytes potentiates antineoplastic immunity attenuating hepatocellular carcinoma proliferation

    This article has 11 authors:
    1. Wei Dong
    2. Guihu Wang
    3. Senyang Li
    4. Yichao Chai
    5. Qian Wang
    6. Yucheng Li
    7. Qiaoman Fei
    8. Yujin Zong
    9. Jing Geng
    10. Pengfei Liu
    11. Zongfang Li
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This valuable study presents an innovative noninvasive immunotherapeutic strategy for hepatocellular carcinoma by combining ultrasound stimulation with calcium-loaded nanodroplets to activate splenic immune responses. The authors provide solid preclinical data, including single-cell transcriptomic analyses and evidence of tumor growth suppression, supported by a creative and well-executed methodology. Further validation of the calcium signaling mechanisms and assessment of long-term safety will strengthen the translational potential of this approach. The work will be of broad interest to researchers in oncology, immunotherapy, and biomedical engineering.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 1 listLatest version Latest activity
  19. The impact of ambient contamination on demultiplexing methods for single-nucleus multiome experiments

    This article has 10 authors:
    1. Terence Li
    2. Marcus Alvarez
    3. Cuining Liu
    4. Kevin Abuhanna
    5. Yu Sun
    6. Jason Ernst
    7. Kathrin Plath
    8. Brunilda Balliu
    9. Chongyuan Luo
    10. Noah Zaitlen
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This study introduces ambisim, a rigorously validated and well-documented simulation framework that enables the generation of synthetic, genotype-aware single-cell RNA and ATAC sequencing datasets under realistic conditions. The authors provide solid evidence of its utility by benchmarking multiple demultiplexing methods and proposing a new variant consistency metric. While the tool is valuable for guiding method selection, the interpretation of the new metric requires further clarification.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  20. Neural Representation of Time across Complementary Reference Frames

    This article has 4 authors:
    1. Yangwen Xu
    2. Nicola Sartorato
    3. Léo Dutriaux
    4. Roberto Bottini
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This study presents a valuable finding on the neural representation of time from two distinct egocentric and allocentric reference frames. The presentation of evidence in the version of the original submission is incomplete, as further conceptual clarifications, methodological details, and addressing potential confounds would strengthen the study. The work will be of interest to cognitive neuroscientists working on the perception and memory of time.

    Reviewed by eLife

    This article has 3 evaluationsAppears in 1 listLatest version Latest activity