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A list by Prachee Avasthi Recommended Reading

See what researchers at Prachee Avasthi’s lab are reading to discover some interesting new work.

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  1. An apical membrane complex controls rhoptry exocytosis and invasion in Toxoplasma

    1. Daniela Sparvoli
    2. Jason Delabre
    3. Diana Marcela Penarete-Vargas
    4. Shrawan Kumar Mageswaran
    5. Lev M. Tsypin
    6. Justine Heckendorn
    7. Liam Theveny
    8. Marjorie Maynadier
    9. Marta Mendonça Cova
    10. Laurence Berry-Sterkers
    11. Amandine Guérin
    12. Jean-François Dubremetz
    13. Serge Urbach
    14. Boris Striepen
    15. Aaron P. Turkewitz
    16. Yi-Wei Chang
    17. Maryse Lebrun
    Appears in 1 listLatest version

  2. Three-dimensional flagella structures from animals’ closest unicellular relatives, the Choanoflagellates

    1. Justine M Pinskey
    2. Adhya Lagisetty
    3. Long Gui
    4. Nhan Phan
    5. Evan Reetz
    6. Amirrasoul Tavakoli
    7. Gang Fu
    8. Daniela Nicastro

    • Curated by eLife

      Evaluation Summary:

      Cryo-electron tomography has been successful at uncovering the details of the eukaryotic cilium/flagellum but there remains a limited number of taxa represented in the studies to date. Pinskey et al. fill this gap with a flagellar structure from choanoflagellates: single-celled organisms that are the closest living relatives of animals. The findings yield many new insights of broad interest to the field, such as the similarity of outer dynein arms and radial spokes to metazoan cilia, the observation of a flagellar vanes, and the presence of mysterious barb structures.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

    Reviewed by eLife

    5 evaluationsAppears in 3 listsLatest version Latest activity

  4. Asymmetric requirement for α-tubulin over β-tubulin

    1. Linnea C. Wethekam
    2. Jeffrey K. Moore
    Appears in 1 listLatest version

    Prachee Avasthi Recommended Reading

    This is a super interesting study on the effects of super-stoichiometric alpha or beta tubulin showing budding yeast can tolerate excess alpha but not beta tubulin for normal mictotubule assembly and function. As usual, it is staggering how much we have yet to learn about the fundamentals of cytoskeletal assembly and regulation!

  5. Septins mediate a microtubule-actin crosstalk that enables actin growth on microtubules

    1. Konstantinos Nakos
    2. Megan R. Radler
    3. Ilona A. Kesisova
    4. Meagan R. Tomasso
    5. Shae B. Padrick
    6. Elias T. Spiliotis
    Appears in 2 listsLatest version

    Prachee Avasthi Recommended Reading

    This is wild! Microtubule-bound septins can capture growing actin filaments for growth along microtubules.

  6. Proteomic analysis of the actin cortex in interphase and mitosis

    1. Neza Vadnjal
    2. Sami Nourreddine
    3. Geneviève Lavoie
    4. Murielle Serres
    5. Philippe P. Roux
    6. Ewa K. Paluch
    Appears in 1 listLatest version

    Prachee Avasthi Recommended Reading

    Cell cortex proteome during morphological changes throughout interphase and cell division!

  7. Actin remodelling controls proteasome homeostasis upon stress

    1. Thomas David Williams
    2. Roberta Cacioppo
    3. Alexander Agrotis
    4. Ailsa Black
    5. Houjiang Zhou
    6. Adrien Rousseau
    Appears in 1 listLatest version

    Prachee Avasthi Recommended Reading

    Very interesting study demonstrating localized translation at yeast endocytic sites of mRNAs for a proteasome assembly-related chaperone during cell stress (rapamycin treatment/TORC1 inhibition). I would be really interested to know if other mRNAs artificially targeted to the same site (which increases translation of this proteasome chaperone) also increases their translation more broadly.

  8. Atypical and distinct microtubule radial symmetries in the centriole and the axoneme of Lecudina tuzetae

    1. Alexandra Bezler
    2. Alexander Woglar
    3. Fabian Schneider
    4. Friso Douma
    5. Léo Bürgy
    6. Coralie Busso
    7. Pierre Gönczy
    Appears in 1 listLatest version

    Prachee Avasthi Recommended Reading

    The 9-fold radial symmetry of centrioles, despite being scaffolded by sas-6 which self assembles in the same pattern, can show different numbers of mictotubules in vivo. This paper show both 8-fold radially symmetric microtubules in centrioles of the parasite Lecudina tuzetae and a reduced number of those 8 that extend into the ciliary axoneme, highlighting other factors that must be involved in regulation of symmetry and selective microtubule extension.

  9. Proteostasis in ice: the role of heat shock proteins and ubiquitin in the freeze tolerance of the intertidal mussel, Mytilus trossulus

    1. Lauren T. Gill
    2. Jessica R. Kennedy
    3. Katie E. Marshall
    Appears in 1 listLatest version

    Prachee Avasthi Recommended Reading

    Getting more interested in cell biological strategies of extremotolerant organisms. Intertidal mussels can survive internal ice formation and sub-zero temps. Interesting result of multiple freeze thaw cycles to allow for heat shock protein expression and ubiquitin conjugation of damaged proteins for recovery between freezes results in reduced mortality in mussels compared to a single freeze.

  10. Unrestrained growth of correctly oriented microtubules instructs axonal microtubule orientation

    1. Maximilian AH Jakobs
    2. Assaf Zemel
    3. Kristian Franze
    Appears in 1 listLatest version

    Prachee Avasthi Recommended Reading

    Incredibly interesting! Proximal axons have mixed microtubule polarity and here, they use fly neurons and simulations to suggest that plus end-out microtubules undergo less catastrophe due to a kinesin-1 dependent p150 gradient that stabilizes distal +ends and promotes plus end-out microtubule growth.

  11. Flagellar energy costs across the tree of life

    1. Paul E Schavemaker
    2. Michael Lynch

    • Curated by eLife

      Evaluation Summary:

      This work will be of interest to readers in the fields of cell biology, evolutionary biology, and biophysics. The collected data are of good quality and are properly analysed. The work thus convincingly demonstrates that energetic considerations (building costs versus potential benefit) must be taken into account to understand flagellar evolution.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

    Reviewed by eLife

    4 evaluationsAppears in 3 listsLatest version Latest activity

    Prachee Avasthi Recommended Reading

    Incredibly cool analysis of energetic costs of flagellar construction and operation in bacteria, archaea, and eukaryotes and proposes that only larger cells can afford the eukaryotic flagellum. Super interesting example of micromonas (tiny algae closer to prokaryote size) that have a minimal eukaryotic flagellum.

  12. A chemical biology toolbox to investigate in-cell target engagement and specificity of PRMT5-inhibitors

    1. Elisabeth M. Rothweiler
    2. Jakub Stefaniak
    3. Jennifer A. Ward
    4. Catherine Rogers
    5. Esra Balikci
    6. Kilian V. M. Huber
    Appears in 1 listLatest version

    Prachee Avasthi Recommended Reading

    Nice method that reads out small molecule interaction with its cellular target in live cells using bioluminescence resonance energy transfer. The target of interest is fused with a luciferase and the small molecule is conjugated to a fluorophore.

  14. Puromycin is incorporated into regenerating flagella of Chlamydomonas reinhardtii as an indicator of nascent flagellar proteins

    1. Tomohiro Kubo
    2. Natsumi Kanou
    3. Toshiyuki Oda
    Appears in 1 listLatest version

    Prachee Avasthi Recommended Reading

    Interesting paper recapitulating the finding that newly synthesized proteins are incorporated at distal tips into Chlamy cilia using puromycin labelling. This is during post-pH shock mediated reassembly. Previously @wallaceUCSF found the distal incorporation using labelled tubulin in dikaryons.

  15. Multiple ciliary localization signals control INPP5E ciliary targeting

    1. Dario Cilleros-Rodriguez
    2. Raquel Martin-Morales
    3. Pablo Barbeito
    4. Abhijit Deb Roy
    5. Abdelhalim Loukil
    6. Belen Sierra-Rodero
    7. Gonzalo Herranz
    8. Olatz Pampliega
    9. Modesto Redrejo-Rodriguez
    10. Sarah C Goetz
    11. Manuel Izquierdo
    12. Takanari Inoue
    13. Francesc R Garcia-Gonzalo

    • Curated by eLife

      Evaluation Summary:

      This manuscript is of interest to developmental and cell biologists exploring cilia dynamics and ciliopathies. The authors address the molecular mechanisms by which INPP5E, a phosphoinositide phosphatase essential for regulating cilia function, is targeted to the primary cilium of cultured mammalian cells. Using immunoprecipitation, ciliary localization, phosphatase activity assays in combination with structural modelling, the authors identify four motifs important for ciliary localization of INPP5E, and uncover several novel and important interactions with other ciliary proteins providing a likely mechanism for ciliary targeting. The claims are generally well supported by the data, but some additional data acquisition and analysis are required to fully support the authors' conclusions and provide a conceptual advance in the field.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their names with the authors.)

    Reviewed by eLife

    4 evaluationsAppears in 3 listsLatest version Latest activity

    Prachee Avasthi Recommended Reading

    Unprecedented complexity and redundancy in ciliary targeting signals of the ciliary phosphoinositide phosphatase, INPP5E.

  17. Actin network architecture can ensure robust centering or sensitive decentering of the centrosome

    1. Shohei Yamamoto
    2. Jérémie Gaillard
    3. Benoit Vianay
    4. Christophe Guerin
    5. Magali Orhant‐Prioux
    6. Laurent Blanchoin
    7. Manuel Théry

    Reviewed by Review Commons

    4 evaluationsAppears in 2 listsLatest version Latest activity

    Prachee Avasthi Recommended Reading

    Another great paper from the CytomorphoLab using a cell-free system to determine the interplay of actin networks and microtubule forces in centrosome positioning.

  18. Amphibian mucus triggers a developmental transition in the frog-killing chytrid fungus

    1. Kristyn A. Robinson
    2. Sarah M. Prostak
    3. Evan H. Campbell Grant
    4. Lillian K. Fritz-Laylin
    Appears in 1 listLatest version

    Prachee Avasthi Recommended Reading

    Awesome study from the @FritzLaylin group on the calcium-dependent encystation of chytrid fungi in response to mucin/mucous.

  19. Comparative Phenotyping of Two Commonly Used Chlamydomonas reinhardtii Background Strains: CC-1690 (21gr) and CC-5325 (The CLiP Mutant Library Background)

    1. Ningning Zhang
    2. Leila Pazouki
    3. Huong Nguyen
    4. Sigrid Jacobshagen
    5. Brae M. Bigge
    6. Ming Xia
    7. Erin M. Mattoon
    8. Anastasiya Klebanovych
    9. Maria Sorkin
    10. Dmitri A. Nusinow
    11. Prachee Avasthi
    12. Kirk J. Czymmek
    13. Ru Zhang
    Appears in 1 listLatest version

    Prachee Avasthi Recommended Reading

    Important characterization of two of our commonly used wild type Chlamy strains.

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