Reading list

A list by Biophysics Colab

Articles that are being read by Biophysics Colab.

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  1. Structure of the lens MP20 mediated adhesive junction

    1. William J. Nicolas
    2. Anna Shiriaeva
    3. Michael W. Martynowycz
    4. Angus C Grey
    5. Yasmeen Ruma
    6. Paul J Donaldson
    7. Tamir Gonen
    Appears in 1 listLatest version

  4. A synthetic method to assay polycystin channel biophysics

    1. Megan Larmore
    2. Orhi Esarte Palomero
    3. Neha Kamat
    4. Paul G DeCaen

    • Curated by eLife

      eLife Assessment

      The authors have developed a valuable approach that employs cell-free expression to reconstitute ion channels into giant unilamellar vesicles for biophysical characterisation. The work is convincing and will be of particular interest to those studying ion channels that primarily occur in organelles and are therefore not amenable to be studied by more traditional methods.

    Reviewed by eLife

    7 evaluationsAppears in 2 listsLatest version Latest activity

  12. Deep Mutagenesis of a Transporter for Uptake of a Non-Native Substrate Identifies Conformationally Dynamic Regions

    1. Heather J Ellis
    2. Matthew Chan
    3. Balaji Selvam
    4. Evan Walter
    5. Christine A Devlin
    6. Steven K Szymanski
    7. Loren Keith Henry
    8. Diwakar Shukla
    9. Erik Procko

    • Curated by eLife

      eLife assessment

      Serotonin is an important neurotransmitter and its synaptic concentration is controlled by re-uptake by the sodium-coupled serotonin transporter SERT. The manuscript by Chan et al reports results from a systematic deep mutagenesis approach to study the surface expression and APP+ (5HT analogue) transport mechanism of the human serotonin transporter. The authors complement this experimental evidence with large-scale molecular simulations of the transporter in the presence of APP+. The use of deep mutagenesis and large-scale adaptive sampling simulations is impressive, and could contribute to understanding the structural requirements for folding and how transporters evolve to recognize different substrates.

    Reviewed by eLife

    5 evaluationsAppears in 2 listsLatest version Latest activity

  19. Ligand-coupled conformational changes in a cyclic nucleotide-gated ion channel revealed by time-resolved transition metal ion FRET

    1. Pierce Eggan
    2. Sharona E Gordon
    3. William N Zagotta

    • Curated by eLife

      eLife Assessment

      This valuable study uses fluorescence lifetime imaging and steady-state and time-resolved transition metal ion FRET to characterize conformational transitions in the isolated cyclic nucleotide binding domain of a bacterial CNG channel. The data are compelling and support the authors' conclusions. The results advance the understanding of allosteric mechanisms in CNBD channels and have theoretical and practical implications for other studies of protein allostery. A limitation is that only the cytosolic fragments of the channel were studied.

    Reviewed by eLife

    10 evaluationsAppears in 2 listsLatest version Latest activity
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