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A list by Biophysics Colab

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  4. Structures and membrane interactions of native serotonin transporter in complexes with psychostimulants

    1. Dongxue Yang
    2. Zhiyu Zhao
    3. Emad Tajkhorshid
    4. Eric Gouaux

    • Curated by Biophysics Colab

      Evaluation statement (24 May 2023)

      Yang et al. present valuable information about ligand interactions with the serotonin transporter SERT, innovatively purified from pig brain using Fab fragments. The approach of using natively expressed SERT is notable for its potential insight into binding of endogenous membrane components such as lipids. Data distinguishing binding of the psychostimulants methamphetamine and cocaine add to our knowledge of substrate and inhibitor interactions with SERT and allow direct comparison with the closely related dopamine transporter DAT. The authors carefully state the limitations of their findings, including the possibility that the monomeric transporter stable in detergent micelles might exist in a multimeric state in native membranes.

      Biophysics Colab considers this to be a convincing study and recommends it to scientists interested in the structure, mechanism and ligand interactions of neurotransmitter transporters.

      (This evaluation by Biophysics Colab refers to version 2 of this preprint, which has been revised in response to peer review of version 1.)

    Reviewed by Biophysics Colab

    3 evaluationsAppears in 4 listsLatest version Latest activity

  8. Improved ANAP incorporation and VCF analysis reveal details of P2X7 current facilitation and a limited conformational interplay between ATP binding and the intracellular ballast domain

    1. Anna Durner
    2. Ellis Durner
    3. Annette Nicke

    • Curated by eLife

      eLife assessment

      This manuscript constitutes an important foray into the conformational rearrangements throughout various domains of the notoriously difficult-to-study P2X7 receptor, with a focus on the enigmatic intracellular 'ballast' domain. This is of broad interest to those studying the role of enzymatically active intracellular domains of membrane proteins. The authors provide convincing evidence that the ballast domain is unlikely to undergo major conformational changes upon ATP-induced gating, but additional experimental support is required on the facilitation process and to elucidate the consequences exerted by intracellular factors.

    Reviewed by eLife

    5 evaluationsAppears in 2 listsLatest version Latest activity

  9. Negative allosteric modulation of the glucagon receptor by RAMP2

    1. Kaavya Krishna Kumar
    2. Evan S. O’Brien
    3. Chris H. Habrian
    4. Naomi R. Latorraca
    5. Haoqing Wang
    6. Inga Tuneew
    7. Elizabeth Montabana
    8. Susan Marqusee
    9. Daniel Hilger
    10. Ehud Y. Isacoff
    11. Jesper Mosolff Mathiesen
    12. Brian K. Kobilka
    Appears in 1 listLatest version

  10. Structures of ferroportin in complex with its specific inhibitor vamifeport

    1. Elena Farah Lehmann
    2. Márton Liziczai
    3. Katarzyna Drożdżyk
    4. Patrick Altermatt
    5. Cassiano Langini
    6. Vania Manolova
    7. Hanna Sundstrom
    8. Franz Dürrenberger
    9. Raimund Dutzler
    10. Cristina Manatschal

    • Curated by eLife

      eLife assessment

      This important study reports cryo-EM structures of human ferroportin (FPN), a protein essential for iron transport in humans. This manuscript will be of interest to researchers studying membrane transport mechanisms as well as to those interested in drug design. The structures detail interactions between FPN and the small-molecule inhibitor vamifeport, which is currently in clinical trials for sickle cell disease, and ta new (occluded) protein conformation that is stabilized by a sybody (a nanobody selected from a synthetic library) is identified. Evidence for the mechanism of inhibition by vamifeport is convincing, but evidence for the physiological relevance of the occluded conformation is still incomplete.

    Reviewed by eLife

    4 evaluationsAppears in 2 listsLatest version Latest activity

  12. Mechanism of Ca2+ transport by ferroportin

    1. Jiemin Shen
    2. Azaan Saalim Wilbon
    3. Ming Zhou
    4. Yaping Pan

    • Curated by eLife

      eLife assessment

      This study makes an important contribution to the function of the iron transporter Ferroportin (Fpn). By using a combination of proteoliposome assays, mutagenesis and structural studies by cryo EM, the authors are able to demonstrate that the H+-driven transporter for Fe2+-efflux is also capable of passive Ca2+ influx. The evidence supporting the conclusions is convincing, but the rate of Ca2+ influx and the physiological relevance of Ca2+ entry is yet to be established. The work will be of broad interest to cell biologists and biochemists.

    Reviewed by eLife

    4 evaluationsAppears in 2 listsLatest version Latest activity

  14. Cryo-EM complex structure of active GPR75 with a nanobody

    1. Zilin Lv
    2. Yuntong He
    3. Yuning Xiang
    4. Jing Li
    5. Shuhao Zhang
    6. Fanhao Meng
    7. Baoliang Lan
    8. Hanbo Guo
    9. Dong He
    10. Yanxia Wang
    11. Huimin Zhao
    12. Wei Zhuo
    13. Yujie Liu
    14. Xiangyu Liu
    15. Xiaodan Ni
    16. Jie Heng
    Appears in 1 listLatest version

  16. A pathogenic human Orai1 mutation unmasks STIM1-independent rapid inactivation of Orai1 channels

    1. Priscilla S-W Yeung
    2. Megumi Yamashita
    3. Murali Prakriya

    • Curated by eLife

      eLife assessment

      This manuscript reports novel and important findings on the mechanisms of regulation of CRAC channels. Collectively, the work represents an important conceptual advancement, showing that stromal interaction molecule-1 is not necessary for Ca2+-dependent inactivation of the Orai1 channel and that Orai1 likely contains a Ca2+ sensor for autoregulatio. The experiments are carefully conducted, and the data is of high quality and support the major conclusions of the authors.

    Reviewed by eLife

    4 evaluationsAppears in 2 listsLatest version Latest activity
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