Reading List

Minute-timescale free-energy calculations reveal a pseudo-active state in the adenosine A2A receptor activation mechanism

1. Vincenzo Maria D’Amore
2. Paolo Conflitti
3. Luciana Marinelli
4. Vittorio Limongelli

Structural and mechanistic insights into human choline and ethanolamine transport

1. Keiken Ri
2. Tsai-Hsuan Weng
3. Ainara Claveras Cabezudo
4. Wiebke Jösting
5. Zhang Yu
6. Andre Bazzone
7. Nancy C.P. Leong
8. Sonja Welsch
9. Raymond T. Doty
10. Gonca Gursu
11. Tiffany Jia Ying Lim
12. Sarah Luise Schmidt
13. Janis L. Abkowitz
14. Gerhard Hummer
15. Di Wu
16. Long N Nguyen
17. Schara Safarian

Fusarium graminearum Ste2 and Ste3 Receptors Undergo Peroxidase Induced Heterodimerization

1. Tanya Sharma
2. Robert Y. Jomphe
3. Dongling Zhang
4. Ana C. Magalhaes
5. Michele C. Loewen

Structure-based discovery of CFTR potentiators and inhibitors

1. Fangyu Liu
2. Anat Levit Kaplan
3. Jesper Levring
4. Jürgen Einsiedel
5. Stephanie Tiedt
6. Katharina Distler
7. Natalie S. Omattage
8. Ivan S. Kondratov
9. Yurii S. Moroz
10. Harlan L. Pietz
11. John J. Irwin
12. Peter Gmeiner
13. Brian K. Shoichet
14. Jue Chen

Human pannexin 1 channel is not phosphorylated by Src tyrosine kinase at Tyr199 and Tyr309

1. Zheng Ruan
2. Junuk Lee
3. Yangyang Li
4. Juan Du
5. Wei Lü

• Curated by eLife

  eLife assessment

  The current manuscript re-examines an established claim in the literature that human PANX-1 is regulated by Src kinase phosphorylation at two tyrosine residues, Y199 and Y309. This issue is important for our understanding of Pannexin channel regulation. The authors present an extensive series of experiments that fail to detect PANX-1 phosphorylation at these sites. Although the authors' approach is more rigorous than the previous studies, this work relies primarily on negative results that are not unambiguously definitive; the work nonetheless provides a compelling reason for the field to reexamine conclusions drawn in earlier studies.

Reviewed by eLife

Activation mechanism of the cardiac calcium pump by a small-molecule allosteric modulator

1. Jaroslava Šeflová
2. Carlos Cruz-Cortés
3. Guadalupe Guerrero-Serna
4. Seth L. Robia
5. L. Michel Espinoza-Fonseca

Oligomeric states of an Influenza-encoded PB1-F2 viroporin

1. Sehrish Jamal
2. Syed Tarique Moin
3. Shozeb Haider

PI-(3,5)P2-mediated oligomerization of the endosomal sodium/proton exchanger NHE9

1. Surabhi Kokane
2. Pascal F. Meier
3. Ashutosh Gulati
4. Rei Matsuoka
5. Tanadet Pipatpolkai
6. Giuseppe Albano
7. Lucie Delemotte
8. Daniel Fuster
9. David Drew

Negative allosteric modulation of the µ-opioid receptor

1. Evan S. O’Brien
2. Vipin Ashok Rangari
3. Amal El Daibani
4. Shainnel O. Eans
5. Betsy White
6. Haoqing Wang
7. Yuki Shiimura
8. Kaavya Krishna Kumar
9. Kevin Appourchaux
10. Weijiao Huang
11. Chensong Zhang
12. Jesper M. Mathiesen
13. Tao Che
14. Jay P. McLaughlin
15. Susruta Majumdar
16. Brian K. Kobilka

Conformational changes in the Niemann–Pick type C1 protein NCR1 drive sterol translocation

1. Kelly M. Frain
2. Emil Dedic
3. Lynette Nel
4. Anastasiia Bohush
5. Esben Olesen
6. Katja Thaysen
7. Daniel Wüstner
8. David L. Stokes
9. Bjørn Panyella Pedersen

Structural mechanisms for VMAT2 inhibition by tetrabenazine

1. Michael P Dalton
2. Mary Hongying Cheng
3. Ivet Bahar
4. Jonathan A Coleman

• Curated by eLife

  eLife assessment

  The report presents the cryo-EM structure of human vesicular monoamine transporter 2 (VMAT2) bound to tetrabenazine, a clinical drug. VMAT2 is critical for neurotransmission, and the study constitutes an important milestone in neurotransmitter transport research. The evidence presented in the report is convincing and provides new opportunities for developing improved therapeutic interventions and furthering our understanding of this vital protein's function.

Reviewed by eLife

Structural mechanisms for VMAT2 inhibition by tetrabenazine

1. Michael P Dalton
2. Mary Hongying Cheng
3. Ivet Bahar
4. Jonathan A Coleman

• Curated by eLife

  eLife assessment

  The report presents the cryo-EM structure of human vesicular monoamine transporter 2 (VMAT2) bound to tetrabenazine, a clinical drug. VMAT2 is critical for neurotransmission, and the study constitutes an important milestone in neurotransmitter transport research. The evidence presented in the report is convincing and provides new opportunities for developing improved therapeutic interventions and furthering our understanding of this vital protein's function.

Reviewed by eLife

Probing the stability and interdomain interactions in the ABC transporter OpuA using single-molecule optical tweezers

1. Lyan van der Sleen
2. Jan A. Stevens
3. Siewert J. Marrink
4. Bert Poolman
5. Kasia Tych

LRMP inhibits cAMP potentiation of HCN4 channels by disrupting intramolecular signal transduction

1. Colin H Peters
2. Rohit K Singh
3. Avery A Langley
4. William G Nichols
5. Hannah R Ferris
6. Danielle A Jeffrey
7. Catherine Proenza
8. John R Bankston

• Curated by eLife

  eLife assessment

  This study identifies the molecular determinants of LRMP co-regulation of HCN 4 activity. The evidence supporting the conclusions, which is compelling, is backed by rigorous electrophysiological and spectroscopic analysis. The work is important because it greatly enhances our understanding of the mechanisms of HCN channel regulation in a tissue-specific manner and highlights a functional role for more disordered regions that have yet to be structurally resolved.

Reviewed by eLife

The mechanism of Gα _q regulation of PLCβ3 -catalyzed PIP2 hydrolysis

1. Maria E. Falzone
2. Roderick MacKinnon

Step-wise activation of a Family C GPCR

1. Kaavya Krishna Kumar
2. Haoqing Wang
3. Chris Habrian
4. Naomi R. Latorraca
5. Jun Xu
6. Evan S. O’Brien
7. Chensong Zhang
8. Elizabeth Montabana
9. Antoine Koehl
10. Susan Marqusee
11. Ehud Y. Isacoff
12. Brian K. Kobilka

Structural and biophysical analysis of a Haemophilus influenzae tripartite ATP-independent periplasmic (TRAP) transporter

1. Michael J Currie
2. James S Davies
3. Mariafrancesca Scalise
4. Ashutosh Gulati
5. Joshua D Wright
6. Michael C Newton-Vesty
7. Gayan S Abeysekera
8. Ramaswamy Subramanian
9. Weixiao Y Wahlgren
10. Rosmarie Friemann
11. Jane R Allison
12. Peter D Mace
13. Michael DW Griffin
14. Borries Demeler
15. Soichi Wakatsuki
16. David Drew
17. Cesare Indiveri
18. Renwick CJ Dobson
19. Rachel A North

• Curated by eLife

  eLife assessment

  The current manuscript presents a cryo-EM structure of a tripartite ATP-independent periplasmic (TRAP) transporter that contributes to Haemophilus influenzae virulence. Convincing biophysical and cryo-EM experiments yield a valuable molecular model, but the functional importance of some of the molecular features identified remains to be demonstrated.

Reviewed by eLife

Structural dynamics at cytosolic inter-protomer interfaces control gating of a mammalian TRPM5 channel

1. Sebastian Karuppan
2. Lynn Goss Schrag
3. Andrés Jara-Oseguera
4. Lejla Zubcevic

Divergent folding-mediated epistasis among unstable membrane protein variants

1. Laura M Chamness
2. Charles P Kuntz
3. Andrew G McKee
4. Wesley D Penn
5. Christopher M Hemmerich
6. Douglas B Rusch
7. Hope Woods
8. Dyotima
9. Jens Meiler
10. Jonathan P Schlebach

• Curated by eLife

  eLife assessment

  This important study describes exhaustive deep mutational scanning (DMS) of the gonadotropin-releasing hormone wild-type receptor and for two single point mutations that impact its folding and structure, monitoring how plasma membrane expression levels are influenced by the introduced mutations. With solid evidence, the authors have pioneered an exploration of the interaction between mutations (epistasis) in a membrane protein, with a potential for explaining membrane protein evolution and genetic diseases.

Reviewed by eLife

A conserved ion channel function of STING mediates non-canonical autophagy and cell death

1. Jinrui Xun
2. Zhichao Zhang
3. Bo Lyu
4. Defen Lu
5. Haoxiang Yang
6. Guijun Shang
7. Jay Xiaojun Tan