eLife

Latest preprint reviews

1. Multi-dimensional leaf phenotypes reflect root system genotype in grafted grapevine over the growing season

   This article has 14 authors:

   1. Zachary N Harris
   2. Mani Awale
   3. Niyati Bhakta
   4. Daniel H Chitwood
   5. Anne Fennell
   6. Emma Frawley
   7. Laura L Klein
   8. Laszlo G Kovacs
   9. Misha Kwasniewski
   10. Jason P Londo
   11. Qin Ma
   12. Zoë Migicovsky
   13. Joel F Swift
   14. Allison J Miller

   This article has been curated by 1 group:

   • Curated by eLife

     Summary: Experimentally, this is a very solid and nicely replicated experimental design that provides a strong ability to interrogate the questions at hand. Both reviewers had a concern that the use of PCs was underpowering the analysis to test the key questions that were the goal of the experiment. The manuscript could also be improved by working to interleave the different omics datasets to develop a deeper insight.

   Reviewed by eLife

2. Cryo-EM structure of the yeast TREX complex and coordination with the SR-like protein Gbp2

   This article has 7 authors:

   1. Yihu Xie
   2. Bradley P Clarke
   3. Yong Joon Kim
   4. Austin L Ivey
   5. Pate S Hill
   6. Yi Shi
   7. Yi Ren

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This is an interesting paper describing the structure of the yeast THO:Sub2 complex and how it interacts with the SR like protein Gbp2. The paper extends what we have learned from two recently published Tho:Sub2 complex structures by the Conti and Plaschka groups in two ways. Firstly, it shows how Gbp2 interacts with the THO complex. Secondly, it reveals a substantially different orientation between THO:Sub2 protomers compared with the earlier structure, so provides more information on the flexibility and range of movements that the two protomers might make with respect to each other. The structural inferences are supported by some biochemical experiments but mechanistically the work has limitations, similar to other recent cryo-EM structures of this complex. However, this is an important structure of wide interest to people working on gene expression in eukaryotes and it undoubtedly advances the field.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers opted to remain anonymous to the authors.)

   Reviewed by eLife

3. Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish

   This article has 3 authors:

   1. Meredith H Wilson
   2. Stephen C Ekker
   3. Steven A Farber

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript has generated novel and useful tools to mark cytoplasmic lipid droplets and monitor their dynamics in various tissues in live animals. It will be of interest to researchers studying lipid metabolism and related human diseases.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

4. A sustained type I IFN-neutrophil-IL-18 axis drives pathology during mucosal viral infection

   This article has 13 authors:

   1. Tania Lebratti
   2. Ying Shiang Lim
   3. Adjoa Cofie
   4. Prabhakar Andhey
   5. Xiaoping Jiang
   6. Jason Scott
   7. Maria Rita Fabbrizi
   8. Ayşe Naz Ozantürk
   9. Christine Pham
   10. Regina Clemens
   11. Maxim Artyomov
   12. Mary Dinauer
   13. Haina Shin

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript will be of interest to a broad audience of immunologists especially those studying host-pathogen interactions, mucosal immunology, innate immunity and interferons. The study reveals a novel role for neutrophils in the regulation of pathological inflammation during viral infection of the genital mucosa. The main conclusions are well supported by a combination of precise technical approaches including neutrophil-specific gene targeting and antibody-mediated inhibition of selected pathways.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

   Reviewed by eLife

5. IL-33 promotes innate lymphoid cell-dependent IFN-γ production required for innate immunity to Toxoplasma gondii

   This article has 10 authors:

   1. Joseph T Clark
   2. David A Christian
   3. Jodi A Gullicksrud
   4. Joseph A Perry
   5. Jeongho Park
   6. Maxime Jacquet
   7. James C Tarrant
   8. Enrico Radaelli
   9. Jonathan Silver
   10. Christopher A Hunter

   Reviewed by eLife

6. Naa12 compensates for Naa10 in mice in the amino-terminal acetylation pathway

   This article has 29 authors:

   1. Hyae Yon Kweon
   2. Mi-Ni Lee
   3. Max Dorfel
   4. Seungwoon Seo
   5. Leah Gottlieb
   6. Thomas PaPazyan
   7. Nina McTiernan
   8. Rasmus Ree
   9. David Bolton
   10. Andrew Garcia
   11. Michael Flory
   12. Jonathan Crain
   13. Alison Sebold
   14. Scott Lyons
   15. Ahmed Ismail
   16. Elaine Marchi
   17. Seong-keun Sonn
   18. Se-Jin Jeong
   19. Sejin Jeon
   20. Shinyeong Ju
   21. Simon J Conway
   22. Taesoo Kim
   23. Hyun-Seok Kim
   24. Cheolju Lee
   25. Tae-Young Roh
   26. Thomas Arnesen
   27. Ronen Marmorstein
   28. Goo Taeg Oh
   29. Gholson J Lyon

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript describes the identification of an animal model that reproduces several features presented in Ogden syndrome patients and reveals the roles of two N-terminal acetyltransferases in mouse development. It will be of interest to the readers in the field of protein acetylation and modification, and also to the scientific community involved in rare diseases and syndrome studies.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

7. Feeding Experimentation Device version 3 (FED3): An open-source home-cage compatible device for measuring food intake and operant behavior

   This article has 25 authors:

   1. Bridget A. Matikainen-Ankney
   2. Thomas Earnest
   3. Mohamed Ali
   4. Eric Casey
   5. Amy K. Sutton
   6. Alex Legaria
   7. Kia Barclay
   8. Laura B. Murdaugh
   9. Makenzie R. Norris
   10. Yu-Hsuan Chang
   11. Katrina P. Nguyen
   12. Eric Lin
   13. Alex Reichenbach
   14. Rachel E. Clarke
   15. Romana Stark
   16. Sineadh M. Conway
   17. Filipe Carvalho
   18. Ream Al-Hasani
   19. Jordan G. McCall
   20. Meaghan C. Creed
   21. Victor Cazares
   22. Matthew W. Buczynski
   23. Michael J. Krashes
   24. Zane Andrews
   25. Alexxai V. Kravitz

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     All three reviewers were very enthusiastic about this manuscript describing FED3, a new and improved open-source option for a home cage pellet dispensing device. They all agreed that this open-source tool would be of wide-interest to neuroscience laboratories, that the manuscript was well-written and clear, and that the cross-lab validation was informative. They also appreciated that this Tools & Resource manuscript all necessary open-source hardware, firmware, visualization code, and Arduino and Python libraries for user customization of experiments and analysis. Minor concerns were identified with the extent to which the manuscript describes and compares to existing systems and with clarity on some details of the system.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

8. Seroconversion stages COVID19 into distinct pathophysiological states

   This article has 18 authors:

   1. Matthew D Galbraith
   2. Kohl T Kinning
   3. Kelly D Sullivan
   4. Ryan Baxter
   5. Paula Araya
   6. Kimberly R Jordan
   7. Seth Russell
   8. Keith P Smith
   9. Ross E Granrath
   10. Jessica R Shaw
   11. Monika Dzieciatkowska
   12. Tusharkanti Ghosh
   13. Andrew A Monte
   14. Angelo D'Alessandro
   15. Kirk C Hansen
   16. Tellen D Benett
   17. Elena WY Hsieh
   18. Joaquín M Espinosa

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     In this study, the authors use a systems immunology approach to document innate and adaptive immune responses during clincal SARS-CoV-2 infection. This general impact of this work is a better understanding of COVID19 pathobiology and more specifically, the identification of serum antibodies as a novel classification framework to understand COVID-19 disease course and associated changes.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife, ScreenIT

9. Sushi domain-containing protein 4 controls synaptic plasticity and motor learning

   This article has 20 authors:

   1. Inés González-Calvo
   2. Keerthana Iyer
   3. Mélanie Carquin
   4. Anouar Khayachi
   5. Fernando A Giuliani
   6. Séverine M Sigoillot
   7. Jean Vincent
   8. Martial Séveno
   9. Maxime Veleanu
   10. Sylvana Tahraoui
   11. Mélanie Albert
   12. Oana Vigy
   13. Célia Bosso-Lefèvre
   14. Yann Nadjar
   15. Andréa Dumoulin
   16. Antoine Triller
   17. Jean-Louis Bessereau
   18. Laure Rondi-Reig
   19. Philippe Isope
   20. Fekrije Selimi

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The reviewers agreed that this is a very interesting paper that demonstrates the involvement of a specific protein degradation pathway in a form of synaptic plasticity in the cerebellum. The strength of the work results from its innovative character. The authors show that SUSD4 is expressed throughout the brain and is abundant in cerebellar dendrites and spines. Mice with deletion of SUSD4 have motor coordination and learning deficits, along with impaired LTD induction. This study provides novel insight in the uncharacterized role of SUSD4 and provides a detailed and well-performed analysis of the Susd4 loss of function phenotype in the cerebellar circuit. The exact mechanism by which SUSD4 affects GluA2 levels remains unclear. However, their findings provide leads for further functional follow-up studies of SUSD4.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

10. TRPM channels mediate learned pathogen avoidance following intestinal distention

    This article has 3 authors:

    1. Adam Filipowicz
    2. Jonathan Lalsiamthara
    3. Alejandro Aballay

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      In this manuscript, the authors aim to address an important and interesting question: when an animal's intestine is colonized by pathogenic bacteria, how can it sense these bacteria and learn to avoid consuming them? Here the authors suggest that in C. elegans, sensing of intestinal distension or bloating caused by Gram-positive bacteria via intestinal ion channels may drive rapid behavioral responses through a process involving associative learning. While these findings are of broad interest to both the microbiology and neurobiology community, some of their conclusions are not currently fully supported by their data, and reasonable alternative interpretations exist.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

    Reviewed by eLife