eLife

Latest preprint reviews

1. Mechanisms underlying neonate-specific metabolic effects of volatile anesthetics

   This article has 14 authors:

   1. Julia Stokes
   2. Arielle Freed
   3. Rebecca Bornstein
   4. Kevin N Su
   5. John Snell
   6. Amanda Pan
   7. Grace X Sun
   8. Kyung Yeon Park
   9. Sangwook Jung
   10. Hailey Worstman
   11. Brittany M Johnson
   12. Philip G Morgan
   13. Margaret M Sedensky
   14. Simon C Johnson

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript reports that Volatile anesthetics VA induce a rapid depletion of circulating ß-HB and the induction of hypoglycemia by VA in neonates, but not in adults. The phenomenon is very interesting and robust, however it has already been described. Whats new here is that through a metabolomics analysis they demonstrate a role of ACC and CPT1 in this phenomenon.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

2. Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development

   This article has 6 authors:

   1. Lucie Zilova
   2. Venera Weinhardt
   3. Tinatini Tavhelidse
   4. Christina Schlagheck
   5. Thomas Thumberger
   6. Joachim Wittbrodt

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The experimental system characterized in this paper opens up new avenues for studying mechanisms of retinal patterning and morphogenesis. The data presented make a compelling case for the emergence of complex multicellular structures upon re-aggregation of embryonic teleost cells, but open questions remain regarding the basic underlying principles.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

3. Enhanced Cas12a multi-gene regulation using a CRISPR array separator

   This article has 4 authors:

   1. Jens P Magnusson
   2. Antonio Ray Rios
   3. Lingling Wu
   4. Lei S Qi

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript is of broad interest to those performing multiplexed genome engineering and related applications with CRISPR Cas12a technologies. While the proposed use of synSeparators is promising, the paper would benefit from further investigation of the mechanism by which synSeparators function to promote Cas12a activity. Additional data would be required to support the current conclusions regarding the generalizability of the findings.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

   Reviewed by eLife

4. SSNA1 stabilizes dynamic microtubules and detects microtubule damage

   This article has 4 authors:

   1. Elizabeth J Lawrence
   2. Goker Arpag
   3. Cayetana Arnaiz
   4. Marija Zanic

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     In this manuscript, Lawrence et al. investigate the direct effects of the microtubule-associated protein, SSNA1, on microtubule dynamics and damage using purified proteins and TIRF microscopy. The authors conclude that SSNA1 is a microtubule stabilizing protein and a sensor of microtubule damage. This paper is of high interest to scientists within the field of microtubule mechanics and of broad interest to scientists studying cilia, cell division and neuronal development.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

5. Apical contacts stemming from incomplete delamination guide progenitor cell allocation through a dragging mechanism

   This article has 9 authors:

   1. Eduardo Pulgar
   2. Cornelia Schwayer
   3. Néstor Guerrero
   4. Loreto López
   5. Susana Márquez
   6. Steffen Härtel
   7. Rodrigo Soto
   8. Carl-Philipp Heisenberg
   9. Miguel L Concha

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     In this study, Pulgar et al. describe an interesting phenomenon addressing organ integrity in a unique example of collective cell migration. The group focused on the migration of the dorsal forunner cells (DFC), which will constitute the left-right organizer of the zebrafish. The authors show that DFCs retain apical contacts stemming from incomplete delamination and drag detached DFCs to their final destination. The study opens a number of exciting new questions related to the mechanism underlying the 'safeguards' process and the mechanism of coordination between migration and regulation of attachment.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

6. High rates of evolution preceded shifts to sex-biased gene expression in Leucadendron, the most sexually dimorphic angiosperms

   This article has 3 authors:

   1. Mathias Scharmann
   2. Anthony G Rebelo
   3. John R Pannell

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This is one of the first studies to investigate sex-biased gene expresion in a broad phylogenetic context, and the first in a plant genus. The findings go against the classical view that sex-biased gene expression is driven by sex-specific selection for sexual dimorphism, and instead suggests that sex-bias preverentially evolved in genes that already had the highest expression variance to begin with. It will broadly appeal to researchers interested in the evolution of sexual dimorphism.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

7. Interpreting wide-band neural activity using convolutional neural networks

   This article has 11 authors:

   1. Markus Frey
   2. Sander Tanni
   3. Catherine Perrodin
   4. Alice O'Leary
   5. Matthias Nau
   6. Jack Kelly
   7. Andrea Banino
   8. Daniel Bendor
   9. Julie Lefort
   10. Christian F Doeller
   11. Caswell Barry

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Frey et al. describe a convolutional neural network capable of extracting behavioral correlates from wide-band LFP recordings or even lower-frequency imaging data. The analysis program described by the authors provides a rapid "first pass" analysis using raw, unprocessed data to generate hypotheses that can be tested later with conventional in-depth analyses. This approach is of real value to the community, particularly as it becomes more commonplace for labs to acquire multi-site in vivo recordings.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

8. A Cas-BCAR3 co-regulatory circuit controls lamellipodia dynamics

   This article has 5 authors:

   1. Elizabeth M Steenkiste
   2. Jason D Berndt
   3. Carissa Pilling
   4. Christopher Simpkins
   5. Jonathan A Cooper

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study focuses on the formation of adaptor protein complexes at adhesion sites and their links to in vitro membrane ruffling and cell movement. Specifically, the authors study the role of the adaptor BCAR3 protein which is regulated by post-translational mechanisms (ubiquitin degradation and tyrosine phosphorylation). The authors propose a "co-regulatory" model whereby the recruitment of BCAR3 to adhesions acts to modulate p130Cas tyrosine phosphorylation and cell migration. This manuscript would be of particular interest to cell and cancer biologists interested in the molecular regulation of cell migration.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

9. A meta-analysis of the association between male dimorphism and fitness outcomes in humans

   This article has 3 authors:

   1. Linda H Lidborg
   2. Catharine Penelope Cross
   3. Lynda G Boothroyd

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This work evaluates the strength of the evidence that human sexual dimorphism is the product of sexual selection. As a meta-analysis of studies that connect various measures of masculinity to various measures of reproductive success, this paper represents a synthesis of what this vast literature can show thus far. The work will be of general interest to evolutionary social scientists from a variety of disciplines, and it does a good job of clearly and concisely presenting the current state of sexual selection research on human males. The data are well presented, but the interpretation of the results is currently limited by some gaps in the theoretical framework guiding the manuscript.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewers #1-4 agreed to share their names with the authors.)

   Reviewed by eLife

10. Single-cell RNA-seq analysis reveals penaeid shrimp hemocyte subpopulations and cell differentiation process

    This article has 7 authors:

    1. Keiichiro Koiwai
    2. Takashi Koyama
    3. Soichiro Tsuda
    4. Atsushi Toyoda
    5. Kiyoshi Kikuchi
    6. Hiroaki Suzuki
    7. Ryuji Kawano

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      This study provides identification of different subpopulations of blood cells and gives new insights in putative hemocyte lineage relationships by single cell RNA sequencing. The main conclusions are fairly well supported by the data and this manuscript will be of high interest to crustacean immunologists and readers in the field of aquaculture.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

    Reviewed by eLife