eLife

Latest preprint reviews

1. METTL3 promotes homologous recombination repair and modulates chemotherapeutic response in breast cancer by regulating the EGF/RAD51 axis

   This article has 9 authors:

   1. Enjie Li
   2. Mingyue Xia
   3. Yu Du
   4. Kaili Long
   5. Feng Ji
   6. Feiyan Pan
   7. Lingfeng He
   8. Zhigang Hu
   9. Zhigang Guo

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The potential mechanism of METTL3 N6-methyltransferase in the chemotherapeutic response is poorly defined. Herein, Li and colleagues describe a pathway where METTL3 promoted EGF expression through m6A modification, which further upregulated RAD51 expression, resulting in enhanced HR activity. METTL3 knockdown results in DNA damage accumulation, which renders breast cancer cells sensitive to adriamycin treatment.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

2. We couldn't get details of this article at this time:
   10.1101/2021.08.28.458037

3. Impairing one sensory modality enhances another by reconfiguring peptidergic signalling in Caenorhabditis elegans

   This article has 2 authors:

   1. Giulio Valperga
   2. Mario de Bono

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript reports a surprising discovery in C. elegans: upon losing its normal sensory properties, the ADL nociceptive/pheromone sensing neuron relays and thereby enhances oxygen behavioral responses via neuropeptide signaling. This effect could be interpreted as cross-modal sensory plasticity or more general a cross-modulation between sensory circuits, a still open question that should be addressed in a revision. The study is relevant to scientists working on sensory neurobiology and neuronal plasticity.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

4. Application of ATAC-Seq for genome-wide analysis of the chromatin state at single myofiber resolution

   This article has 7 authors:

   1. Korin Sahinyan
   2. Darren M Blackburn
   3. Marie-Michelle Simon
   4. Felicia Lazure
   5. Tony Kwan
   6. Guillaume Bourque
   7. Vahab D Soleimani

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     In this paper, Sahinyan and colleagues developed a method for analyzing chromatin accessibility in single murine myofibers. This goal was achieved by adapting the previously published OMNI-ATAC protocol to the specific properties of the myofiber environment. To demonstrate the validity of this method, they isolated myofibers from uninjured and regenerating murine EDL muscles dissected from wild type animals. In a second experiment, this method was applied to isolate myofibers from mdx mice, a model of Duchenne Muscular Dystrophy. The resulting datasets were further compared to the one generated from purified muscle stem cells.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

5. Reversing chemorefraction in colorectal cancer cells by controlling mucin secretion

   This article has 7 authors:

   1. Gerard Cantero-Recasens
   2. Josune Alonso-Marañón
   3. Teresa Lobo-Jarne
   4. Marta Garrido
   5. Mar Iglesias
   6. Lluis Espinosa
   7. Vivek Malhotra

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The study by Cantero-Recasens et al. aims to investigate if mucus secreted by colorectal cancers would impact the effect of the frequently used chemotherapy treatment, FOLFIRI as it has been reported that mucinous carcinomas are more treatment resistant. They further investigate the role of some mucus secretion regulatory genes in this context. The conclusions made on the effect of the mucus secretion regulatory genes are well supported, though the link to the function of mucus in reducing treatment availability needs some further clarifications.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

6. A novel mechanism of bulk cytoplasmic transport by cortical dynein in Drosophila ovary

   This article has 4 authors:

   1. Wen Lu
   2. Margot Lakonishok
   3. Anna S Serpinskaya
   4. Vladimir I Gelfand

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     In their manuscript, Lu et al. use a combination of experimental approaches to determine how cellular components are transported from nurse cells into the growing oocyte during Drosophila egg development. The authors demonstrate that the minus-end directed microtubule motor, dynein, generates cortical flow by gliding microtubules along the cell cortex. This flow is capable of propelling cargoes through the ring canals into the growing oocyte via a bulk cytoplasmic transport mechanism. This action is distinct from dynein's cargo transport functions, as the authors are able to replace dynein with a minus-end directed kinesin linked to the cortex and observe the same phenomenon. Overall, this work had broad significance to cell biologists and developmental biologists interested in intracellular transport functions and oocyte development.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

7. Thymocytes trigger self-antigen-controlling pathways in immature medullary thymic epithelial stages

   This article has 8 authors:

   1. Noella Lopes
   2. Nicolas Boucherit
   3. Jérémy C Santamaria
   4. Nathan Provin
   5. Jonathan Charaix
   6. Pierre Ferrier
   7. Matthieu Giraud
   8. Magali Irla

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript will be of interest to readers in the field of immunology and especially in the induction of immune tolerance in the thymus. The work uses several mouse models to substantially broaden the current understanding of MHCII/TCR -mediated cell-cell crosstalk in the thymus and suggests a novel mechanism that contributes to the generation of functional and self-tolerant T-cells.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

8. Patient-specific Boolean models of signalling networks guide personalised treatments

   This article has 12 authors:

   1. Arnau Montagud
   2. Jonas Béal
   3. Luis Tobalina
   4. Pauline Traynard
   5. Vigneshwari Subramanian
   6. Bence Szalai
   7. Róbert Alföldi
   8. László Puskás
   9. Alfonso Valencia
   10. Emmanuel Barillot
   11. Julio Saez-Rodriguez
   12. Laurence Calzone

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper presents a mathematical model for prioritizing drugs for prostate cancer patients based on signal network database. The manuscript is of broad interest to the field of oncology and precision medicine. The methodology developed is sophisticated and relevant to real patient prostate cancer data. The predictions from the model are validated in an experimental setting and provide suggestions for the personalisation of prostate cancer treatment. The study can serve as a roadmap for future development of predictive, personalized models.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

9. Adaptation in cone photoreceptors contributes to an unexpected insensitivity of primate On parasol retinal ganglion cells to spatial structure in natural images

   This article has 4 authors:

   1. Zhou Yu
   2. Maxwell H Turner
   3. Jacob Baudin
   4. Fred Rieke

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript provides strong evidence that adaptation in cone photoreceptors of the primate retina can subtly change the balance of excitatory and inhibitory inputs to On parasol ganglion cells and thereby fundamentally affect how these cells integrate visual information. This study provides important mechanistic insight into the previous observation that On parasol cells display nonlinear spatial stimulus integration under standard reversing gratings but linearly integrate signals in the context of natural scenes. The findings will be of great interest to visual neuroscientists.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

10. Biophysical Kv3 channel alterations dampen excitability of cortical PV interneurons and contribute to network hyperexcitability in early Alzheimer’s

    This article has 8 authors:

    1. Viktor J Olah
    2. Annie M Goettemoeller
    3. Sruti Rayaprolu
    4. Eric B Dammer
    5. Nicholas T Seyfried
    6. Srikant Rangaraju
    7. Jordane Dimidschstein
    8. Matthew JM Rowan

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      Using computational modeling and dynamic clamp recordings, this work supports the concept that hyperexcitability of cortical circuits in a familial mouse model of Alzheimer's disease is caused by impairments of biophysical properties of Kv3 channels in parvalbumin-positive cortical interneurons. Overall, the work is clear and interesting but some further analysis is required to provide compelling support to the central claims.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

    Reviewed by eLife