eLife

Latest preprint reviews

1. Whole-brain connectivity atlas of glutamatergic and GABAergic neurons in the mouse dorsal and median raphe nuclei

   This article has 15 authors:

   1. Zhengchao Xu
   2. Zhao Feng
   3. Mengting Zhao
   4. Qingtao Sun
   5. Lei Deng
   6. Xueyan Jia
   7. Tao Jiang
   8. Pan Luo
   9. Wu Chen
   10. Ayizuohere Tudi
   11. Jing Yuan
   12. Xiangning Li
   13. Hui Gong
   14. Qingming Luo
   15. Anan Li

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This work from Xu et al. uses state of the art viral tracing technologies in Cre-transgenic mouse lines to map the inputs and outputs of glutamatergic and GABAergic neuronal populations in the dorsal (DR) and median raphe (MR) nucleus. A large amount of high-quality anatomical dataset was collected with the advanced fMOST whole-brain imaging system. Data analysis was thorough with significant scientific insights. All figures are of high quality. Overall, this study nicely complements previously published work on whole-brain connectivity of the DR and MR which have chiefly focused on the main neuromodulatory neurons found in these nuclei, i.e. serotonin and dopamine neurons, and will be a valuable contribution to understanding neural circuits of the raphe system.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

2. HBO1-MLL interaction promotes AF4/ENL/P-TEFb-mediated leukemogenesis

   This article has 10 authors:

   1. Satoshi Takahashi
   2. Akinori Kanai
   3. Hiroshi Okuda
   4. Ryo Miyamoto
   5. Yosuke Komata
   6. Takeshi Kawamura
   7. Hirotaka Matsui
   8. Toshiya Inaba
   9. Akifumi Takaori-Kondo
   10. Akihiko Yokoyama

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript describes the identification and characterization of the interaction between MLL fusion proteins with the HBO1 histone acetyltransferase complex and its role in leukemogenesis. This study adds mechanistic depth into the important recent discovery of HBO1 functions in MLL-fusion leukemias and opens possibilities for a new therapeutic approach.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

3. Delilah, prospero, and D-Pax2 constitute a gene regulatory network essential for the development of functional proprioceptors

   This article has 9 authors:

   1. Adel Avetisyan
   2. Yael Glatt
   3. Maya Cohen
   4. Yael Timerman
   5. Nitay Aspis
   6. Atalya Nachman
   7. Naomi Halachmi
   8. Ella Preger-Ben Noon
   9. Adi Salzberg

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The study is carefully performed and provides compelling evidence for a gene network involved in generating different sensory cell types from a common progenitor, showing how an enhancer can integrate a gene regulatory network and guide a cell-fate decision.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

4. Neutrophil-mediated oxidative stress and albumin structural damage predict COVID-19-associated mortality

   This article has 20 authors:

   1. Mohamed A Badawy
   2. Basma A Yasseen
   3. Riem M El-Messiery
   4. Engy A Abdel-Rahman
   5. Aya A Elkhodiry
   6. Azza G Kamel
   7. Hajar El-sayed
   8. Asmaa M Shedra
   9. Rehab Hamdy
   10. Mona Zidan
   11. Diaa Al-Raawi
   12. Mahmoud Hammad
   13. Nahla Elsharkawy
   14. Mohamed El Ansary
   15. Ahmed Al-Halfawy
   16. Alaa Elhadad
   17. Ashraf Hatem
   18. Sherif Abouelnaga
   19. Laura L Dugan
   20. Sameh Saad Ali

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Severe COVID-19 is characterised by a high neutrophil to lymphocyte ratio and an underlying state of oxidative stress in blood has also been hypothesized. In the study presented here for the first time provide evidence that hydrogen peroxide generated by the neutrophil specific enzyme myeloperoxidase is not only accumulated in plasma but also perpetrates structural damage to the strong and weak lipid binding sites on albumin, a key antioxidant in blood plasma, using SLFAs and EPR spectroscopy. This study is timely and relevant in deciding treatment of severe COVID-19 patients with human serum albumin and possibly supplementation of FDA approved antioxidants like glutathione. The main strengths of the manuscript are the novelty. The main weak point is the need to study more patients.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife, ScreenIT

5. Mutation analysis links angioimmunoblastic T-cell lymphoma to clonal hematopoiesis and smoking

   This article has 4 authors:

   1. Shuhua Cheng
   2. Wei Zhang
   3. Giorgio Inghirami
   4. Wayne Tam

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper provides supportive sequencing data for the hypothesis of association between AITL and pre-existing clonal hematopoiesis. It will be of interest to researchers in both lymphoma and myeloid malignancies and raises new hypotheses about the potential smoking-related mechanisms (C>A signature) that may contribute to development of AITL in the background of clonal hematopoiesis. The hypotheses need further validation, as the authors used a small sample and make indirect inferences about the origin of the observed mutations.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

   Reviewed by eLife

6. Correction of amblyopia in cats and mice after the critical period

   This article has 5 authors:

   1. Ming-fai Fong
   2. Kevin R Duffy
   3. Madison P Leet
   4. Christian T Candler
   5. Mark F Bear

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study reports that monocular inactivation of the fellow (good) eye with tetrodotoxin supports long-lasting recovery from the effects of monocular deprivation, as measured by visual evoked potentials in primary visual cortex. This work should be of interest to neuroscientists studying plasticity and clinicians treating amblyopia. The results are compelling, although the advance compared to previous work is incremental.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

7. Engineering paralog-specific PSD-95 recombinant binders as minimally interfering multimodal probes for advanced imaging techniques

   This article has 18 authors:

   1. Charlotte Rimbault
   2. Christelle Breillat
   3. Benjamin Compans
   4. Estelle Toulmé
   5. Filipe Nunes Vicente
   6. Monica Fernandez-Monreal
   7. Patrice Mascalchi
   8. Camille Genuer
   9. Virginia Puente-Muñoz
   10. Isabel Gauthereau
   11. Eric Hosy
   12. Stéphane Claverol
   13. Gregory Giannone
   14. Ingrid Chamma
   15. Cameron D Mackereth
   16. Christel Poujol
   17. Daniel Choquet
   18. Matthieu Sainlos

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The precise, simple and non-interfering visualization of neuronal key structures is a major challenge and currently limiting the advancement of our understanding of brain function. This work presents intrabodies as selective and non-interfering tools for the visualization of PSD95 - a major scaffold of the neuronal excitatory postsynapse. This is an important and well executed work that provides an excellent new tool to study an important synaptic molecule.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

8. Comparative transcriptomic analysis reveals translationally relevant processes in mouse models of malaria

   This article has 6 authors:

   1. Athina Georgiadou
   2. Claire Dunican
   3. Pablo Soro-Barrio
   4. Hyun Jae Lee
   5. Myrsini Kaforou
   6. Aubrey J Cunnington

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     In this work, the authors use independent public datasets to perform an unbiased investigation of the similarities and differences of mouse models to human malarial disease using comparative transcriptomics. Whilst the data cannot convincingly identify which mouse models are best suited for studying specific human malaria phenotypes, the comparative analyses do indicate that these models reflect the broad diversity of human disease.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

9. Regulation of positive and negative selection and TCR signaling during thymic T cell development by capicua

   This article has 6 authors:

   1. Soeun Kim
   2. Guk-Yeol Park
   3. Jong Seok Park
   4. Jiho Park
   5. Hyebeen Hong
   6. Yoontae Lee

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper focuses on the transcriptional regulation of the T cell receptor (TCR) signaling cascade and would be of interest to those studying T cell development and differentiation. The authors employ a conditional deletion of the Capicua (Cic) gene, a transcriptional repressor previously shown to be involved in regulating autoimmunity and follicular helper T (Tfh) cell differentiation, and now show that loss of CIC in hematopoietic cells leads to defects in TCR-beta selection as well as in positive and negative selection of developing thymocytes. The overall conclusions are well supported by the findings.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

10. Spatial transcriptomic and single-nucleus analysis reveals heterogeneity in a gigantic single-celled syncytium

    This article has 11 authors:

    1. Tobias Gerber
    2. Cristina Loureiro
    3. Nico Schramma
    4. Siyu Chen
    5. Akanksha Jain
    6. Anne Weber
    7. Anne Weigert
    8. Malgorzata Santel
    9. Karen Alim
    10. Barbara Treutlein
    11. J Gray Camp

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      Single celled organisms are assumed to be smaller, simpler, and less complex than multicellular organisms like animals. However, there are many examples of large single-celled protists - especially amoeba - that can be up to centimeters in size, and it remains unclear how they are able to achieve these sizes and differentiated regions like tissues in animals. Here, the authors provide evidence for variation in gene expression in the syncytial (multinucleate) large amoeba Physarum polycephalum. While primarily descriptive work, the authors are claiming a provocative mechanistic interpretation of the single cell gene expression results, but not yet supported by the current data. This study is neverhteless elegant and interesting regarding heterogeneity of gene expression patterns and thus specialization of functions within a syncytial organism.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

    Reviewed by eLife