eLife

Latest preprint reviews

1. Phox2b mutation mediated by Atoh1 expression impaired respiratory rhythm and ventilatory responses to hypoxia and hypercapnia

   This article has 8 authors:

   1. Caroline B Ferreira
   2. Talita M Silva
   3. Phelipe E Silva
   4. Claudio L Castro
   5. Catherine Czeisler
   6. José J Otero
   7. Ana C Takakura
   8. Thiago S Moreira

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study provides a novel mouse model for the study of the central respiratory chemoreceptor circuit and, therefore, of interest for the respiratory physiology community. Nonetheless, in its present form, this work still lacks more physiological, developmental, and anatomical characterizations to place this study in a broader context and gain new insights into the physiology of respiratory chemoreflexes.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife

2. Structure and ion-release mechanism of PIB-4-type ATPases

   This article has 17 authors:

   1. Christina Grønberg
   2. Qiaoxia Hu
   3. Dhani Ram Mahato
   4. Elena Longhin
   5. Nina Salustros
   6. Annette Duelli
   7. Pin Lyu
   8. Viktoria Bågenholm
   9. Jonas Eriksson
   10. Komal Umashankar Rao
   11. Domhnall Iain Henderson
   12. Gabriele Meloni
   13. Magnus Andersson
   14. Tristan Croll
   15. Gabriela Godaly
   16. Kaituo Wang
   17. Pontus Gourdon

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper presents crystal structures of sCoaT, a heavy metal transporting P-type ATPase. These structures and complementary functional data define the overall fold of this protein and provide insight into several mechanistic features, including a conserved histidine proposed to act as a novel counter-ion during transport. The study will be of interest to biochemists and microbiologists interested in the transport of transition metals, structural biology of membrane proteins and drug development.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

3. Molecular reconstruction of recurrent evolutionary switching in olfactory receptor specificity

   This article has 3 authors:

   1. Lucia L Prieto-Godino
   2. Hayden R Schmidt
   3. Richard Benton

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study investigates evolutionary changes in ligand preference that occur in an olfactory receptor (IR75a) across the Drosophila phylogeny. The authors find that IR75a displays different odor preferences, for acetic acid or butyric acid, across Drosophila species, and link odor preference to particular protein mutations in the receptor. Reconstruction of a putative ancestral IR75a revises the timeline for IR75a evolution, and structural modeling suggests how mutations alter odor preference.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

4. Chemoreceptor co-expression in Drosophila melanogaster olfactory neurons

   This article has 12 authors:

   1. Darya Task
   2. Chun-Chieh Lin
   3. Alina Vulpe
   4. Ali Afify
   5. Sydney Ballou
   6. Maria Brbic
   7. Philipp Schlegel
   8. Joshua Raji
   9. Gregory SXE Jefferis
   10. Hongjie Li
   11. Karen Menuz
   12. Christopher J Potter

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     A combination of methods. including a new method for tagging genes, demonstrates that the chemosensory co-receptors of Drosophila melanogaster (Orco, IR8a, IR25a, IR76b) are expressed widely and highly overlapping. These findings challenge a long-standing dogma in the field and suggest that different types of receptors, i.e. olfactory and ionotropic receptors, can be co-expressed in the same chemosensory neuron. Moreover, optogenetics and single sensillum recordings provide evidence that IR25a co-receptor might modulate the activity of typical Orco-dependent olfactory sensory neurons.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

5. Social selectivity and social motivation in voles

   This article has 5 authors:

   1. Annaliese K Beery
   2. Sarah A Lopez
   3. Katrina L Blandino
   4. Nicole S Lee
   5. Natalie S Bourdon

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper introduces a new method to measure motivation to engage with familiar or unfamiliar individuals in prairie voles, a widely used animal model system for studying social relationships. The authors show that female prairie voles will work harder to access both familiar pair-bonded males or familiar females. In contrast, male prairie voles will work to access both pair-bonded females as well as unfamiliar females. These results cast a new light on decades of work based partner-preference tests that assess pair bonds that do not assess the role of motivation.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

6. The anterior cingulate cortex and its role in controlling contextual fear memory to predatory threats

   This article has 4 authors:

   1. Miguel Antonio Xavier de Lima
   2. Marcus Vinicius C Baldo
   3. Fernando A Oliveira
   4. Newton Sabino Canteras

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The manuscript used a naturalistic task where mice were fear conditioned to a context using a live predator (cat) and a variety of behavioural measures including freezing, risk assessment, and exploration. The identification of anterior cingulate cortex and its input and outputs in contextual fear acquisition and expression to predator threat is an important contribution to our understanding of neural mechanism related to fear processing. The paper will be of interest to researchers interested in using naturalistic threats in the lab, and to a more broad audience interested in learning and the related fear circuits.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

7. High-resolution mapping of the period landscape reveals polymorphism in cell cycle frequency tuning

   This article has 6 authors:

   1. Zhengda Li
   2. Shiyuan Wang
   3. Meng Sun
   4. Minjun Jin
   5. Daniel Khain
   6. Qiong Yang

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper uses microfluidics and Xenopus extracts to investigate the effects of mitotic feedbacks on the cell cycle period. It is shown that the inhibition of G2 regulatory positive feedback loops does not reduce the tunability of the cell cycle oscillations, while interference with PP2A phosphatase can completely block of the cell cycle. The experiments are well-conducted and the results should be of interest to researchers interested in the cell cycle, specifically in the regulation of mitosis.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

8. ZHX2 promotes HIF1α oncogenic signaling in triple-negative breast cancer

   This article has 18 authors:

   1. Wentong Fang
   2. Chengheng Liao
   3. Rachel Shi
   4. Jeremy M Simon
   5. Travis S Ptacek
   6. Giada Zurlo
   7. Youqiong Ye
   8. Leng Han
   9. Cheng Fan
   10. Lei Bao
   11. Christopher Llynard Ortiz
   12. Hong-Rui Lin
   13. Ujjawal Manocha
   14. Weibo Luo
   15. Yan Peng
   16. William Y Kim
   17. Lee-Wei Yang
   18. Qing Zhang

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study identifies ZHX2 as an oncogenic factor in triple negative breast cancer (TNBC), which interferes with hypoxia-related regulators and accounts for cancer aggressiveness and poor prognosis. The authors show that ZHX2 interacts with HIF1α and increases the expression of its downstream targets and identify ZHX2 residues critical for regulating its activity. This work provides a potential novel target in TNBC treatment and would be of interest to cancer biology researchers.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

9. Temperature evolution following joint loading promotes chondrogenesis by synergistic cues via calcium signaling

   This article has 8 authors:

   1. Naser Nasrollahzadeh
   2. Peyman Karami
   3. Jian Wang
   4. Lida Bagheri
   5. Yanheng Guo
   6. Philippe Abdel-Sayed
   7. Lee Laurent-Applegate
   8. Dominique P Pioletti

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This is the study to determine the effects of mechanical loading on temperature changes in the joint and how the mechano-thermal transduction may influence chondrocyte behavior. This manuscript will be of interest to the clinicians and researchers who are working on tissue engineering and cartilage regeneration. The study has high clinical relevance. It provides new evidence that the mechanical stimuli plus with the temperature increase could influence the cell chondrogenic response. The data support the conclusions of the manuscript within the current context, although several issues need to be addressed.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

10. Clp protease and antisense RNA jointly regulate the global regulator CarD to mediate mycobacterial starvation response

    This article has 10 authors:

    1. Xinfeng Li
    2. Fang Chen
    3. Xiaoyu Liu
    4. Jinfeng Xiao
    5. Binda T Andongma
    6. Qing Tang
    7. Xiaojian Cao
    8. Shan-Ho Chou
    9. Michael Y Galperin
    10. Jin He

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      CarD is an RNA polymerase interacting protein that is essential for mycobacterial viability, the levels of which are important for controlling gene expression in mycobacteria during various stress conditions. This study reports two mechanisms that regulate levels of CarD under stress conditions, including starvation. The authors report that CarD levels are tightly regulated and that there was a dramatic decrease in the levels of CarD when cells switched from the nutrient-rich to the starvation condition. They discovered two synergistic mechanisms that led to this dramatic decrease in CarD. The first is SigF-dependent induction of antisense RNA of CarD (AscarD), which inhibits CarD translation and a second mechanism involving Clp protease-mediated degradation of intracellular CarD. The work will be of interest to researchers studying non-coding RNAs, microbial gene expression, physiology and stress response.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

    Reviewed by eLife