eLife

Latest preprint reviews

1. Magnetic stimulation allows focal activation of the mouse cochlea

   This article has 7 authors:

   1. Jae-Ik Lee
   2. Richard Seist
   3. Stephen McInturff
   4. Daniel J Lee
   5. M Christian Brown
   6. Konstantina M Stankovic
   7. Shelley Fried

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study provides a demonstration that magnetic stimulation of the cochlea is feasible and suggests it could be more precise than electrical stimulation for cochlear implants. The conclusions of the paper are mostly well supported by data, but some aspects of the experimental procedure, the neuronal response acquisition, and the data analysis need to be clarified and extended.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

2. Macrophage inflammation resolution requires CPEB4-directed offsetting of mRNA degradation

   This article has 12 authors:

   1. Clara Suñer
   2. Annarita Sibilio
   3. Judit Martín
   4. Chiara Lara Castellazzi
   5. Oscar Reina
   6. Ivan Dotu
   7. Adrià Caballé
   8. Elisa Rivas
   9. Vittorio Calderone
   10. Juana Díez
   11. Angel R Nebreda
   12. Raúl Méndez

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study examined the role of CEBP4 in resolution of immune inflammatory responses. The manuscript uses genetic and pharmacologic approaches to demonstrate requirement of CEBP4 for survival following LPS administration and outlines certain downstream details of the mechanism. However, certain conclusions pertaining to this mechanism are either weak or not fully clarified. Further, the study proposes that RNA-binding proteins CPEB4 and TTP play important roles in regulating inflammation-associated mRNA transcripts by binding to CPEs or AREs to promote RNA stability or degradation. There is general agreement that most of the claims in the paper appear reasonably well-supported by the experimental data. However, there are some concerns regarding the robustness and significance of the presented data and conclusions as indicated in the individual reviews that require revision.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors).

   Reviewed by eLife

3. Generation of a CRF1-Cre transgenic rat and the role of central amygdala CRF1 cells in nociception and anxiety-like behavior

   This article has 9 authors:

   1. Marcus M Weera
   2. Abigail E Agoglia
   3. Eliza Douglass
   4. Zhiying Jiang
   5. Shivakumar Rajamanickam
   6. Rosetta S Shackett
   7. Melissa A Herman
   8. Nicholas J Justice
   9. Nicholas W Gilpin

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript describes, characterizes, and validates a novel transgenic tool that will be useful for the study of stress neurobiology and the function of the stress-related neuropeptide corticotropin releasing factor. This tool will be especially relevant to the study of stress and related fields, such as addiction, in which rats are a critical model system. There is comprehensive histochemical and functional validation of the novel rat that broadly supports its proposed utility for visualizing and providing access to central amygdala CRF1 receptor-expressing neurons.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

4. Distinct representations of body and head motion are dynamically encoded by Purkinje cell populations in the macaque cerebellum

   This article has 2 authors:

   1. Omid A Zobeiri
   2. Kathleen E Cullen

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Zobeiri and Cullen address the important question of how the cerebellum transforms multiple streams of sensory information into an estimate of the motion of the body in the world. They find that Purkinje cells, the inhibitory principal neurons of the cerebellar cortex, have multimodal and highly diverse responses to vestibular and neck proprioceptive inputs. Notably, this information is combined in a way that is different than what is seen in downstream fastigial neurons, which reflect either head or body motion, but not both. The experiments are well executed, generating data that provide important and novel insights, but there are shortcomings in the model put forward to account for these results.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

5. Automated, high-dimensional evaluation of physiological aging and resilience in outbred mice

   This article has 9 authors:

   1. Zhenghao Chen
   2. Anil Raj
   3. GV Prateek
   4. Andrea Di Francesco
   5. Justin Liu
   6. Brice E Keyes
   7. Ganesh Kolumam
   8. Vladimir Jojic
   9. Adam Freund

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Chen et al. develop a comprehensive platform to score aging-dependent changes in mouse physiology and behavior using a multi-dimensional longitudinal phenotyping approach. Their thorough data collection and analysis reveals a diversity of trajectories in aging-related physiological and behavioral changes and helps disentangle biological aging from chronological aging, providing a reference pioneering work for future studies aimed at large-scale aging multi-dimensional phenotyping.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

6. Palatal morphology predicts the paleobiology of early salamanders

   This article has 3 authors:

   1. Jia Jia
   2. Guangzhao Li
   3. Ke-Qin Gao

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper is of interest to vertebrate paleontologists and other evolutionary biologists interested in the early evolution of amphibians. Using geometric morphometric analysis, the authors demonstrate that both the shape of the palate and several non-shape variables (particularly associated with vomerine teeth) are ecologically informative in early stem- and basal crown-group salamanders. The phylomorphospace analysis reveals that metamorphosis is significant in the expansion of ecomorphospace of the palate in early salamanders. In overall, the main claims of the manuscript are well supported by the data.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1, Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

7. Unbiased proteomics, histochemistry, and mitochondrial DNA copy number reveal better mitochondrial health in muscle of high-functioning octogenarians

   This article has 10 authors:

   1. Ceereena Ubaida-Mohien
   2. Sally Spendiff
   3. Alexey Lyashkov
   4. Ruin Moaddel
   5. Norah J MacMillan
   6. Marie-Eve Filion
   7. Jose A Morais
   8. Tanja Taivassalo
   9. Luigi Ferrucci
   10. Russell T Hepple

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study analyzed muscle protein differences between octogenarian master athletes and non-athletes. The data showed that high physical function in octogenarians was associated with the increased mitochondrial proteome, reduced number of muscle fibers impaired by oxphos, and higher mtDNA copy number. The authors propose that this is one of the mechanisms contributing to better performance in master athletes compared with non-athletes, suggesting that mitochondrial health in skeletal muscle is a key feature in inducing improved physical function in the elderly. This article has the potential to generate a significant impact within the field and will be of interest to a broad audience.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

8. In vivo MRI is sensitive to remyelination in a nonhuman primate model of multiple sclerosis

   This article has 15 authors:

   1. Maxime Donadieu
   2. Nathanael J Lee
   3. María I Gaitán
   4. Seung-Kwon Ha
   5. Nicholas J Luciano
   6. Snehashis Roy
   7. Benjamin Ineichen
   8. Emily C Leibovitch
   9. Cecil C Yen
   10. Dzung L Pham
   11. Afonso C Silva
   12. Mac Johnson
   13. Steve Jacobson
   14. Pascal Sati
   15. Daniel S Reich

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Reich and colleagues have combined MRI imaging and histopathology to study the remyelination of brain lesions in an EAE marmoset model of multiple sclerosis. This work addresses in a non-human primate a missing link in the neuropathology of myelin repair, because in human MS it is virtually impossible to study the lesion dynamics by MRI (in live patients) and demyelination by histology (upon brain autopsy). The present manuscript would be improved by adding further histological evidence of remyelination and clarifying open questions of data acquisition.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

9. Evolution and regulation of microbial secondary metabolism

   This article has 9 authors:

   1. Guillem Santamaria
   2. Chen Liao
   3. Chloe Lindberg
   4. Yanyan Chen
   5. Zhe Wang
   6. Kyu Rhee
   7. Francisco Rodrigues Pinto
   8. Jinyuan Yan
   9. Joao B Xavier

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Santamaria et al. provide interesting insights into the complex regulation used by 31 Pseudomonas aeruginosa clinical strains to minimize the individual costs of cooperative phenotypes based on secondary metabolites. The data analysis is sound and of remarkable depth. Their results challenge the view that there is a tradeoff between primary and secondary metabolism in bacteria and that instead, secondary metabolites may be produced in low-stress conditions when excess carbon is available. However, the relevance of the laboratory growth conditions for these clinical strains requires additional justification.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

10. Mammals adjust diel activity across gradients of urbanization

    This article has 20 authors:

    1. Travis Gallo
    2. Mason Fidino
    3. Brian Gerber
    4. Adam A Ahlers
    5. Julia L Angstmann
    6. Max Amaya
    7. Amy L Concilio
    8. David Drake
    9. Danielle Gay
    10. Elizabeth W Lehrer
    11. Maureen H Murray
    12. Travis J Ryan
    13. Colleen Cassady St Clair
    14. Carmen M Salsbury
    15. Heather A Sander
    16. Theodore Stankowich
    17. Jaque Williamson
    18. J Amy Belaire
    19. Kelly Simon
    20. Seth B Magle

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      This study will be of interest to wildlife ecologists and conservation practitioners. The authors took a collaborative approach and collated a large dataset of wildlife camera trap recordings across cities in the USA. The analyses reveal variability in diel activity among species and cities, providing important insights into the effects of urbanization.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

    Reviewed by eLife