eLife

Latest preprint reviews

1. Brain-wide analysis of the supraspinal connectome reveals anatomical correlates to functional recovery after spinal injury

   This article has 9 authors:

   1. Zimei Wang
   2. Adam Romanski
   3. Vatsal Mehra
   4. Yunfang Wang
   5. Matthew Brannigan
   6. Benjamin C Campbell
   7. Gregory A Petsko
   8. Pantelis Tsoulfas
   9. Murray G Blackmore

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This work seeks to resolve questions surrounding "unexplained variability in functional recovery" after experimental spinal cord injury in mice using virus-based retrograde tracing from cervical, thoracic and lumbar spinal cord injection sites, tissue clearing and cutting-edge imaging, to develop a supraspinal connectome or map of neurons in the brain that project to the spinal cord. They apply their methods to understand the differences in the connections between the brain and the cervical or lumbar spinal cord and to compare the connectome from intact mice to those of mice with mild, moderate and severe spinal cord injuries. This work will be of interest to neuroscientists interested in tissue clearing, viral labelling, and its applications to spinal cord injury.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

2. Cross-modality synthesis of EM time series and live fluorescence imaging

   This article has 4 authors:

   1. Anthony Santella
   2. Irina Kolotuev
   3. Caroline Kizilyaprak
   4. Zhirong Bao

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper very nicely tackles a methodological problem in aligning different types of datasets (EM and light microscopy) to image embryonic nervous system development in the nematode C.elegans. The paper is important not just from a methodological standpoint, but also provides novel insights into nervous system development that will be of general interest to the reader. The latter deserves more attention in the manuscript.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

3. Prenatal development of neonatal vocalizations

   This article has 6 authors:

   1. Darshana Z Narayanan
   2. Daniel Y Takahashi
   3. Lauren M Kelly
   4. Sabina I Hlavaty
   5. Junzhou Huang
   6. Asif A Ghazanfar

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper will be of great interest to the field of developmental neuroscience and social communication. The authors identified prenatal sensorimotor vocal precursors by detecting rhythmic orofacial movements related to vocalizations. These findings will provide new insights into the development of vocal behavior in primates. The data acquired by a highly quantitative approach support the major claims of the paper.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

   Reviewed by eLife

4. Two enhancer binding proteins activate σ54-dependent transcription of a quorum regulatory RNA in a bacterial symbiont

   This article has 9 authors:

   1. Ericka D Surrett
   2. Kirsten R Guckes
   3. Shyan Cousins
   4. Terry B Ruskoski
   5. Andrew G Cecere
   6. Denise A Ludvik
   7. C Denise Okafor
   8. Mark J Mandel
   9. Tim I Miyashiro

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The manuscript has the potential to transform the field of sensory transduction and gene regulation in the Vibrio genus by uncovering a previously undescribed enhancer binding protein and its role in the regulation of quorum sensing and physiology in the Vibrio - squid symbiosis. However, in its present form, several experiments are required to support the claims of the manuscript.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

5. Neuron-associated macrophage proliferation in the sensory ganglia is associated with peripheral nerve injury-induced neuropathic pain involving CX3CR1 signaling

   This article has 14 authors:

   1. Rafaela M Guimarães
   2. Conceição E Aníbal-Silva
   3. Marcela Davoli-Ferreira
   4. Francisco Isaac F Gomes
   5. Atlante Mendes
   6. Maria CM Cavallini
   7. Miriam M Fonseca
   8. Samara Damasceno
   9. Larissa P Andrade
   10. Marco Colonna
   11. Cyril Rivat
   12. Fernando Q Cunha
   13. José C Alves-Filho
   14. Thiago M Cunha

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Guimaraes et al address the origin of the macrophage increase in sensory ganglia after peripheral nerve injury. The authors show that there is no major influx by blood-derived monocytes into ganglia after injury and that resident macrophages proliferate, which is dependent on CX3CR1 signaling. Overall the work is clear and sound and should be of interest to immunologists and neurobiologists.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

   Reviewed by eLife

6. ABCA1 causes an asymmetric cholesterol distribution to regulate intracellular cholesterol homeostasis

   This article has 2 authors:

   1. Fumihiko Ogasawara
   2. Kazumitsu Ueda

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     The mechanisms that control cholesterol movement from the plasma membrane (PM) to the endoplasmic reticulum (ER) remain poorly understood. Here, Ogasawara and Ueda propose an intriguing mechanism whereby ABCA1, a membrane protein, moves cholesterol from the inner to the outer leaflet of the PM to keep the cholesterol away from intracellular Aster proteins that move cholesterol to the ER. When cholesterol builds up beyond a threshold, it accumulates on the inner leaflet and is transported to ER by Asters. If strengthened by the analysis of the endogenous ABCA1 and more physiological cholesterol manipulation, this work will be of significant interest to scientists studying lipid metabolism and transport.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

7. The interpretation of computational model parameters depends on the context

   This article has 6 authors:

   1. Maria Katharina Eckstein
   2. Sarah L Master
   3. Liyu Xia
   4. Ronald E Dahl
   5. Linda Wilbrecht
   6. Anne GE Collins

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Eckstein and colleagues take a within-participant approach to answer two critical questions in the field of human reinforcement learning: to what extent do estimated computational model parameters generalize across different tasks and can their meaning be interpreted in the same way in different task contexts? The authors find that inferred parameters show moderate to little generalizability across tasks, and that their interpretation strongly depends on task context. Support for these claims could be further strengthened through additional simulations and by providing greater methodological detail.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

   Reviewed by eLife

8. Differentiation signals from glia are fine-tuned to set neuronal numbers during development

   This article has 5 authors:

   1. Anadika R Prasad
   2. Inês Lago-Baldaia
   3. Matthew P Bostock
   4. Zaynab Housseini
   5. Vilaiwan M Fernandes

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This manuscript will be of interest to cell and developmental biologists and neuroscientists. It addresses the question of how the number of connecting neurons in a circuit is matched whilst maintaining topography. It shows that non-autonomous control of neuronal number involves a relay mechanism through two distinct glial cell types, enabling the specification of distinct neuronal classes.

     This manuscript was co-submitted with: https://www.biorxiv.org/content/10.1101/2022.02.21.481306v1

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

9. Variable paralog expression underlies phenotype variation

   This article has 9 authors:

   1. Raisa Bailon-Zambrano
   2. Juliana Sucharov
   3. Abigail Mumme-Monheit
   4. Matthew Murry
   5. Amanda Stenzel
   6. Anthony T Pulvino
   7. Jennyfer M Mitchell
   8. Kathryn L Colborn
   9. James T Nichols

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     In this elegant genetic study, Bailon-Zambrano and colleagues draw on classical genetic concepts to address the clinically pertinent question of how genetic variants in the same gene can yield wildly different phenotypes in different individuals. From their case study they conclude that a major contributor is variation in paralog expression. The question addressed is of great interest to evolutionary and developmental biologists in general and to those studying the evolution of developmental mechanisms in particular.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript.The reviewers remained anonymous to the authors.)

   Reviewed by eLife

10. Perception of a conserved family of plant signalling peptides by the receptor kinase HSL3

    This article has 10 authors:

    1. Jack Rhodes
    2. Andra-Octavia Roman
    3. Marta Bjornson
    4. Benjamin Brandt
    5. Paul Derbyshire
    6. Michele Wyler
    7. Marc W Schmid
    8. Frank LH Menke
    9. Julia Santiago
    10. Cyril Zipfel

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      Beginning with transcriptome data, Rhodes et al. identify a new family of peptides with signalling function called CTNIP in the model plant Arabidopsis thaliana. They use an elegant biochemical capture approach to pinpoint the SERK-dependent LRR receptor kinase HSL3 as the only receptor for these peptides. They provide convincing genetic and biochemical evidence that HSL3 binds CTNIP and that CTNIP perception triggers HSL3-dependent cytoplasmic calcium influx, ROS production and transcriptional changes. Furthermore, they provide initial evidence that the CTNIP-HSL3 module may participate in regulating root growth.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

    Reviewed by eLife