eLife

Latest preprint reviews

1. TRF2-mediated ORC recruitment underlies telomere stability upon DNA replication stress

   This article has 6 authors:

   1. Mitsunori Higa
   2. Yukihiro Matsuda
   3. Jumpei Fujii
   4. Nozomi Sugimoto
   5. Kazumasa Yoshida
   6. Masatoshi Fujita

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper is of interest to biochemists studying DNA replication and genome maintenance in eukaryotic cells. The work details a structure-function analysis of an interaction between two proteins that are critical for genome stability. A mutation that disrupts this interaction may have no adverse effects under unperturbed conditions but causes telomeric DNA damage when cells experience replication stress. However, the structural nature of the damage and cellular consequences are not sufficiently explored.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

2. Suppression of endothelial miR-22 mediates non-small cell lung cancer cell-induced angiogenesis

   This article has 13 authors:

   1. Yuan Gu
   2. Gianni Pais
   3. Vivien Becker
   4. Christina Körbel
   5. Emmanuel Ampofo
   6. Elke Ebert
   7. Johannes Hohneck
   8. Nicole Ludwig
   9. Eckart Meese
   10. Rainer M. Bohle
   11. Yingjun Zhao
   12. Michael D. Menger
   13. Matthias W. Laschke

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study reports a novel function of non-coding RNA miR-22 in the regulation of tumor-associated angiogenesis. The presented data suggest a possible link between microRNA and endothelial cell function. If the underlying mechanisms were further explored, this work would be interesting for people working on microRNA function in endothelial cells. Furthermore, considering the increasing interest of combination of anti-angiogenesis agents with anti-PD1 immunotherapy, this work may attract readers interested in immunology.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

3. CRISPR interference identifies vulnerable cellular pathways with bactericidal phenotypes in Mycobacterium tuberculosis

   This article has 5 authors:

   1. Matthew B. McNeil
   2. Laura M. Keighley
   3. Josephine R. Cook
   4. Chen‐Yi Cheung
   5. Gregory M. Cook

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     High-throughput approaches that accurately assess drug target vulberbility in Mycobacterium tuberculosis, the causative agent of tuberculosis, are urgently needed to develop new treatment options for this dreaded disease. This paper applies a CRISPRi based approach to investigate gene essentiality and vulnerability on a diverse set of 96 genes. While the key observations of the study support previous findings, the approach reported here is useful for identification and characterization of novel drug targets. The study will be of interest to microbiologists and those interested in diverse aspects of bacterial metabolism.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

4. Physically interacting beta-delta pairs in the regenerating pancreas revealed by single-cell sequencing

   This article has 11 authors:

   1. Eran Yanowski
   2. Nancy S. Yacovzada
   3. Eyal David
   4. Amir Giladi
   5. Diego Jaitin
   6. Lydia Farack
   7. Adi Egozi
   8. Danny Ben-Zvi
   9. Shalev Itzkovitz
   10. Ido Amit
   11. Eran Hornstein

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper provides an attempt to understand the crosstalk between to islet cell types during beta cell regeneration following partial pancreatectomy. It combines lineage tracing, single cell sequencing and light microscopy to describe islet cell heterogeneity and interactions in the regenerating mouse pancreas. The concept of protective signaling resulting from the direct interactions between beta and delta cells is compelling and would be of interest to scientists in the field of endocrine pancreas development and regeneration. However, the conclusions derived from the sequencing data require additional experimental support.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

5. The SON RNA splicing factor is required for intracellular trafficking structures that promote centriole assembly and ciliogenesis

   This article has 5 authors:

   1. Alexander J. Stemm-Wolf
   2. Eileen T. O’Toole
   3. Ryan M. Sheridan
   4. Jacob T. Morgan
   5. Chad G. Pearson

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This study investigates how deficiency in the RNA splicing factor SON impairs centriole assembly, which may underlie ciliopathy-like phenotypes in humans with SON mutations and is thus of interest to both cell biologists and clinicians. Using RNA-sequencing analysis and advanced imaging techniques the authors discover a large number of known and new SON splicing targets and attempt to identify those crucial for SON knockdown defects. However, knockdown of a subset of targets did not fully recapitulate SON depletion phenotypes and only led to the relatively vague conclusion that the observed centriole assembly defects were caused by impaired protein trafficking around the centrosome.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

6. The Nse5/6-like SIMC1-SLF2 complex localizes SMC5/6 to viral replication centers

   This article has 10 authors:

   1. Martina Oravcová
   2. Minghua Nie
   3. Nicola Zilio
   4. Shintaro Maeda
   5. Yasaman Jami-Alahmadi
   6. Eros Lazzerini-Denchi
   7. James A Wohlschlegel
   8. Helle D Ulrich
   9. Takanori Otomo
   10. Michael N Boddy

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper will be of interest to the chromosome biology field and SMC researchers in particular. The study provides cell biological, biochemical, and structural modeling evidence that a new Nse5-like protein named SIMC1 is a paralog of SLF1, and that the two compete for SLF2-Smc5/6 binding. The authors also show that SIMC1 targets SMC5/6 to polyomavirus replication centers through its SUMO binding motifs (SIMs), supporting a role for SIMC1 in Smc5/6 recruitment for viral restriction.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

   Reviewed by eLife

7. Deconstructing cold-induced brown adipocyte neogenesis in mice

   This article has 5 authors:

   1. Rayanne B Burl
   2. Elizabeth Ann Rondini
   3. Hongguang Wei
   4. Roger Pique-Regi
   5. James G Granneman

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     Burl and Rondini et al. elucidate the transcriptional profile of the stromal vascular fraction of murine brown adipose tissue in the context of thermogenic stimulation. The authors combined systems and reductionist approaches to show the reliance of mature brown adipocytes on adrenergic activation to indirectly stimulate progenitor proliferation and differentiation and the involvement of dendritic cells in this process. Overall, this is a timely and well-rounded work that will provide beneficial data for public use and further resolve the complexities underlying brown adipose physiology.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their name with the authors.)

   Reviewed by eLife

8. MicroRNA-eQTLs in the developing human neocortex link miR-4707-3p expression to brain size

   This article has 9 authors:

   1. Michael J Lafferty
   2. Nil Aygün
   3. Niyanta K Patel
   4. Oleh Krupa
   5. Dan Liang
   6. Justin M Wolter
   7. Daniel H Geschwind
   8. Luis de la Torre-Ubieta
   9. Jason L Stein

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     This paper is one of the first demonstrations that expression quantitative trait loci (eQTL) affecting human microRNAs are linked to brain development affecting brain structure and function. These findings will have a broad impact on the genomics, neural development, and microRNA fields. The datasets produced here (developmental changes in miRNAs, new human miRNAs) will likely be used for further discoveries. However, some claims need to be tempered.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

9. The termination of UHRF1-dependent PAF15 ubiquitin signaling is regulated by USP7 and ATAD5

   This article has 16 authors:

   1. Ryota Miyashita
   2. Atsuya Nishiyama
   3. Weihua Qin
   4. Yoshie Chiba
   5. Satomi Kori
   6. Norie Kato
   7. Chieko Konishi
   8. Soichiro Kumamoto
   9. Hiroko Kozuka-Hata
   10. Masaaki Oyama
   11. Yoshitaka Kawasoe
   12. Toshiki Tsurimoto
   13. Tatsuro S Takahashi
   14. Heinrich Leonhardt
   15. Kyohei Arita
   16. Makoto Nakanishi

   This article has been curated by 1 group:

   • Curated by eLife

     Evaluation Summary:

     DNA methylation inheritance through the UHRF1-DNMT1 signaling axis is becoming increasingly appreciated as a ubiquitin-regulated process. This study builds on the observation that UHRF1 multi mono-ubiquitinates the PCNA-associated protein PAF15, and that, similarly to H3 substrates, these mono-ubiquitin sites are bound by DNMT1 and may contribute to its S-phase chromatin association. The authors focus on players involved in ubiquitin removal and PAF15 release from chromatin and they identify the deubiquitinase USP7 and the DNA replication regulator ATAD5 as important to this termination process. While manipulation of these factors using Xenopus egg extracts shows quite striking effects on DNMT1 chromatin association, effects on DNA methylation are minimal and this brings to question the importance and potential impact of the pathway involving PAF15. In addition, how the findings from Xenopus egg extracts translate to regulation of DNA methylation maintenance in mammalian cells is currently unclear.

     (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

   Reviewed by eLife

10. Insulin sensitivity is preserved in mice made obese by feeding a high starch diet

    This article has 17 authors:

    1. Amanda E Brandon
    2. Lewin Small
    3. Tuong-Vi Nguyen
    4. Eurwin Suryana
    5. Henry Gong
    6. Christian Yassmin
    7. Sarah E Hancock
    8. Tamara Pulpitel
    9. Sophie Stonehouse
    10. Letisha Prescott
    11. Melkam A Kebede
    12. Belinda Yau
    13. Lake-Ee Quek
    14. Greg M Kowalski
    15. Clinton R Bruce
    16. Nigel Turner
    17. Gregory J Cooney

    This article has been curated by 1 group:

    • Curated by eLife

      Evaluation Summary:

      This study evaluates the effects of two distinct dietary methods that cause obesity in mice (high fat vs high starch) on insulin sensitivity and glucose homeostasis. Through a series of nicely performed physiology experiments, the authors demonstrated that high starch feeding causes obesity without deleterious effects on insulin sensitivity. This work will have an impact in the field and help define the important lipid mediators of metabolic disease.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

    Reviewed by eLife