eLife

Latest preprint reviews

1. AGS3 antagonizes LGN to balance oriented cell divisions and cell fate choices in mammalian epidermis

   This article has 9 authors:

   1. Carlos P Descovich
   2. Kendall J Lough
   3. Akankshya Jena
   4. Jessica J Wu
   5. Jina Yom
   6. Danielle C Spitzer
   7. Manuela Uppalapati
   8. Katarzyna M Kedziora
   9. Scott E Williams

   This article has been curated by 1 group:

   • Curated by eLife

     **eLife assessment
     **
     Descovich et al examine the important decision between proliferative (planar) and differentiation (perpendicular) divisions in the basal layers of the skin and find a key promoter of perpendicular divisions is inhibited by its paralog to specify planar divisions. The authors use sophisticated mouse genetics and imaging and discover that LGN and its paralog AGS3 function antagonistically in determining perpendicular vs. planar divisions. Some statements need to be tamed or further backed up, but overall this study provides a significant advance in the field.

   Reviewed by eLife

2. Structure–function analysis of Lactiplantibacillus plantarum DltE reveals D-alanylated lipoteichoic acids as direct cues supporting Drosophila juvenile growth

   This article has 13 authors:

   1. Nikos Nikolopoulos
   2. Renata C Matos
   3. Stephanie Ravaud
   4. Pascal Courtin
   5. Houssam Akherraz
   6. Simon Palussiere
   7. Virginie Gueguen-Chaignon
   8. Marie Salomon-Mallet
   9. Alain Guillot
   10. Yann Guerardel
   11. Marie-Pierre Chapot-Chartier
   12. Christophe Grangeasse
   13. François Leulier

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This is an important study on the role of a bacterial cell wall component, D-alanylated lipoteichoic acid, as a bacteria cue in Drosophila melanogaster-microbiome interactions. Overall, the evidence supporting the conclusions is compelling, with a solid approach combining crystallography with biochemical and cellular assays, that take advantage of both fly and bacterial mutants, to demonstrate a physiological role in juvenile growth promotion. The work will be of broad interest to those studying host-microbe interactions, especially as it is related to immunology and metabolism mediated by the microbiome.

   Reviewed by eLife

3. ERK3/MAPK6 dictates CDC42/RAC1 activity and ARP2/3-dependent actin polymerization

   This article has 6 authors:

   1. Katarzyna Bogucka-Janczi
   2. Gregory Harms
   3. Marie-May Coissieux
   4. Mohamed Bentires-Alj
   5. Bernd Thiede
   6. Krishnaraj Rajalingam

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The manuscript describes a fundamental study of the atypical MAPK, ERK3, in the activation of RhoGTPase Cdc42 and the formation of actin-rich protrusions and cell migration. The results show that ERK3 is required for the motility of tumor cells in vivo, providing a new target for fighting metastasis.

   Reviewed by Arcadia Science, eLife

4. Pupil size reflects activation of subcortical ascending arousal system nuclei during rest

   This article has 4 authors:

   1. Beth Lloyd
   2. Lycia D de Voogd
   3. Verónica Mäki-Marttunen
   4. Sander Nieuwenhuis

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     These are important findings that show that pupil size is not only governed by the locus coeruleus but also by other neuromodulatory subcortical systems. Furthermore, the authors demonstrate that using a standard hemodynamic response kernel is not appropriate for capturing the activity of these systems, at least at rest. Thus, this paper presents compelling evidence against two prevalent working assumptions among researchers in the field.

   Reviewed by eLife

5. Dynamic regulation of inter-organelle communication by ubiquitylation controls skeletal muscle development and disease onset

   This article has 18 authors:

   1. Arian Mansur
   2. Remi Joseph
   3. Euri S Kim
   4. Pierre M Jean-Beltran
   5. Namrata D Udeshi
   6. Cadence Pearce
   7. Hanjie Jiang
   8. Reina Iwase
   9. Miroslav P Milev
   10. Hashem A Almousa
   11. Elyshia McNamara
   12. Jeffrey Widrick
   13. Claudio Perez
   14. Gianina Ravenscroft
   15. Michael Sacher
   16. Philip A Cole
   17. Steven A Carr
   18. Vandana A Gupta

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important work utilizes a model organism, zebrafish, to explore changes to the proteome and the role of KLHL40, a component of the ubiquitin-proteasome system, in the development of skeletal muscle disease. Using mass spectrometry, the authors demonstrate a major and selective role for proteome remodeling in development. They identify a specific role for KLHL40 deletion in regulating the expression of Sar1 - a key component of biosynthetic secretion, where the resulting elevated levels of Sar1 expression potentially lead to collagen secretion defects in the disease state. The findings are incomplete as further experimental characterization of the overall morphological changes and secretion defects, in particular ones derived from the deregulation of Sar1 levels, is required.

   Reviewed by eLife

6. The mutational signatures of poor treatment outcomes on the drug-susceptible Mycobacterium tuberculosis genome

   This article has 5 authors:

   1. Yiwang Chen
   2. Qi Jiang
   3. Mijiti Peierdun
   4. Howard E Takiff
   5. Qian Gao

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     In this useful study, a GWAS-type analysis is applied to clinical Mycobacterium tuberculosis isolates to discover genetic polymorphisms linked to poor tuberculosis outcomes. The evidence for the detected associations is still incomplete, as the corresponding polymorphisms are not adequate to power a prediction model for infection outcome, although key host factors - including patient age, sex, and duration of diagnostic delay (which have stronger predictive value) - appear to enhance predictive capacity.

   Reviewed by eLife

7. Single-cell transcriptomic atlas of lung microvascular regeneration after targeted endothelial cell ablation

   This article has 9 authors:

   1. Rafael Soares Godoy
   2. Nicholas D Cober
   3. David P Cook
   4. Emma McCourt
   5. Yupu Deng
   6. Liyuan Wang
   7. Kenny Schlosser
   8. Katelynn Rowe
   9. Duncan J Stewart

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment:

     The manuscript by Godoy and colleagues is an important contribution to the understanding of how lung endothelial regeneration progresses following endothelial ablation. The novelty and elegance of this study are rooted in the regional and specific ablation of lung endothelial cells using diphtheria toxin without the massive inflammatory activation that is seen with lung injury induced by bacterial infections, viral infections, or lipopolysaccharide. The data convincingly demonstrate that there is an emergence of a highly proliferative lung endothelial subpopulation that drives endothelial regeneration. The translational implications of the study include the identification of potential therapeutic targets to augment endothelial regeneration as a treatment for ALI/ARDS. This study will be of interest to vascular biologists, lung biologists, and researchers studying adult tissue regeneration.

   Reviewed by eLife

8. Proteomic characteristics reveal the signatures and the risks of T1 colorectal cancer metastasis to lymph nodes

   This article has 12 authors:

   1. Aojia Zhuang
   2. Aobo Zhuang
   3. Yijiao Chen
   4. Zhaoyu Qin
   5. Dexiang Zhu
   6. Li Ren
   7. Ye Wei
   8. Pengyang Zhou
   9. Xuetong Yue
   10. Fuchu He
   11. Jianmin Xu
   12. Chen Ding

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This is an important contribution to colorectal cancer research to understand how we can predict high-risk patients for recurrence. The strength of the evidence is solid and looks at a novel approach but an approach that still has opportunities to improve.

   Reviewed by eLife

9. Impact of social isolation on grey matter structure and cognitive functions: A population-based longitudinal neuroimaging study

   This article has 11 authors:

   1. Laurenz Lammer
   2. Frauke Beyer
   3. Melanie Luppa
   4. Christian Sanders
   5. Ronny Baber
   6. Christoph Engel
   7. Kerstin Wirkner
   8. Markus Loffler
   9. Steffi G Riedel-Heller
   10. Arno Villringer
   11. A Veronica Witte

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study provides an important demonstration that loneliness is associated with smaller hippocampal volume, reduced cortical thickness, and worse cognition in healthy older adults. This has theoretical or practical implications beyond a single subfield. The strength of evidence is solid given the cross-sectional and longitudinal design with a few weaknesses. With the analytical and interpretational part strengthened, this paper would be of interest to gerontologists, and dementia/cognitive aging researchers.

   Reviewed by eLife

10. Microtubule-mediated GLUT4 trafficking is disrupted in insulin-resistant skeletal muscle

    This article has 13 authors:

    1. Jonas R Knudsen
    2. Kaspar W Persson
    3. Carlos Henriquez-Olguin
    4. Zhencheng Li
    5. Nicolas Di Leo
    6. Sofie A Hesselager
    7. Steffen H Raun
    8. Janne R Hingst
    9. Raphaël Trouillon
    10. Martin Wohlwend
    11. Jørgen FP Wojtaszewski
    12. Martin AM Gijs
    13. Thomas Elbenhardt Jensen

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      This paper provides strong evidence for the important point that microtubule function is required for the proper localization of Glut4 glucose transporters in an insulin responsive compartment. This membrane localization is required in turn for effective translocation of Glut4 to the muscle cell surface in response to the hormone.

    Reviewed by eLife