eLife

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1. The title and authors for this article are not available from our external data provider yet:
   10.48550/arxiv.2305.04587v2

2. Purine nucleosides replace cAMP in allosteric regulation of PKA in trypanosomatid pathogens

   This article has 9 authors:

   1. Veronica Teresa Ober
   2. George Boniface Githure
   3. Yuri Volpato Santos
   4. Sidney Becker
   5. Gabriel Moya Munoz
   6. Jérôme Basquin
   7. Frank Schwede
   8. Esben Lorentzen
   9. Michael Boshart

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This landmark study sheds light on a long-standing puzzle in Protein kinase A activation in Trypanosoma. Extensive experimental work provides exceptional evidence for the conclusions of the work, which represents a significant advancement in our understanding of the molecular mechanism of cyclic nucleotide binding domains. The work is relevant for researchers with interests in kinases and their mechanistic study.

   Reviewed by eLife

3. Exonuclease Xrn1 regulates TORC1 signaling in response to SAM availability

   This article has 4 authors:

   1. Madeline M. McGinnis
   2. Benjamin M. Sutter
   3. Samira Jahangiri
   4. Benjamin P. Tu

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study in budding yeast (S. cerevisiae) presents important findings demonstrating that the exonuclease Xrn1 regulates autophagy in response to methionine deprivation through effects on TORC1. There is solid evidence that the impact of Xrn1 on TORC1 is contingent on its catalytic activity rather than the degradation of any specific category of mRNAs. A major strength is the novel mechanism, in which Xrn1 modulates the nucleotide-binding state of the Gtr1/2 complex.

   Reviewed by eLife

4. Effect of α-tubulin acetylation on the doublet microtubule structure

   This article has 9 authors:

   1. Shun Kai Yang
   2. Shintaroh Kubo
   3. Corbin Steven Black
   4. Katya Peri
   5. Daniel Dai
   6. Thibault Legal
   7. Melissa Valente-Paterno
   8. Jacek Gaertig
   9. Khanh Huy Bui

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This fundamental study employs a combination of cryo-electron microscopy, molecular dynamics, and mass spectrometry to elucidate the role of α-tubulin acetylation at the lumenal lysine 40 residue (αK40) within the cilium. Compelling evidence shows αK40 acetylation to impact the structure and stability of doublet microtubules in cilia by affecting the lateral rotational angle. The work will be of relevance to those interested in cytoskeleton and structural biology.

   Reviewed by eLife

5. FBXO24 modulates mRNA alternative splicing and MIWI degradation and is required for normal sperm formation and male fertility

   This article has 6 authors:

   1. Zhiming Li
   2. Xingping Liu
   3. Yan Zhang
   4. Yuanyuan Li
   5. Liquan Zhou
   6. Shuiqiao Yuan

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study provides insights into the role of FBXO24 in controlling spermiogenesis and male fertility in mice. The mouse models used and the data are convincing. This paper will interest biomedical researchers working on reproductive biology and fertility control.

   Reviewed by eLife

6. Cell chirality reversal through tilted balance between polymerization of radial fibers and clockwise-swirling of transverse arcs

   This article has 12 authors:

   1. Hoi Kwan Kwong
   2. Miu Ling Lam
   3. Siying Wu
   4. Cho Fan Chung
   5. Jianpeng Wu
   6. Lok Ting Chu
   7. King Hoo Lim
   8. Hiu Lam Chow
   9. Hogi Hartanto
   10. Wengang Liu
   11. Kwan Ting Chow
   12. Ting-Hsuan Chen

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The intrinsic chirality of actin filaments (F-actin) is implicated in the chiral arrangement and movement of cellular structures, but it was unknown how opposite chiralities can arise when the chirality of F-actin is invariant. Kwong et al. present evidence that two actin filament-based cytoskeletal structures, transverse actin arcs and radial stress fibers, drive clockwise and anti-clockwise rotation, respectively. This fundamental work, which has broad implications for cell biology, is supported by compelling data.

   Reviewed by eLife

7. MCAK recognizes the nucleotide-dependent feature at growing microtubule ends

   This article has 4 authors:

   1. Wei Chen
   2. Yin-Long Song
   3. Jian-Feng He
   4. Xin Liang

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This work presents valuable new information on the microtubule-binding mode of the microtubule kinesin-13, MCAK, the authors use quantitative single-molecule studies to propose that MCAK preferentially binds to a GDP-Pi-tubulin portion of the microtubule end. However, the evidence provided to support this claim remains incomplete and would benefit from more rigorous methodology particularly the diffraction limited experiments do not provide sufficient spatial resolution to support the authors' conclusions. In addition, a more through discussion of the existing literature would further strengthen the manuscript.

   Reviewed by eLife

8. Surprising features of nuclear receptor interaction networks revealed by live-cell single-molecule imaging

   This article has 6 authors:

   1. Liza Dahal
   2. Thomas GW Graham
   3. Gina M Dailey
   4. Alec Heckert
   5. Robert Tjian
   6. Xavier Darzacq

   This article has been curated by 1 group:

   • Curated by eLife

     eLife Assessment

     This important study provides data that challenges the standard model that binding of Type 2 Nuclear Receptors to chromatin is limited by the available pool of their common heterodimerization partner Retinoid X Receptor. The evidence supporting the conclusions is compelling, utilizing state-of-the-art single-molecule microscopy. This work will be of broad interest to cell biologists who wish to determine limiting factors in gene regulatory networks.

   Reviewed by eLife

9. Structural insights into the orthosteric inhibition of P2X receptors by non-ATP analog antagonists

   This article has 8 authors:

   1. Danqi Sheng
   2. Chen-Xi Yue
   3. Fei Jin
   4. Yao Wang
   5. Muneyoshi Ichikawa
   6. Ye Yu
   7. Chang-Run Guo
   8. Motoyuki Hattori

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study by Sheng and colleagues provides valuable insights into the mechanism of competitive inhibitors of P2X receptors. The structural and functional evidence supporting the subtype specificity of pyridoxal-5'-phosphate derivatives is compelling and provides information for designing drugs that selectively target different subtypes of P2X receptor channels. The work will be of interest to biochemists, structural biologists, and pharmacologists.

   Reviewed by eLife

10. The Listeria monocytogenes persistence factor ClpL is a potent stand-alone disaggregase

    This article has 10 authors:

    1. Valentin Bohl
    2. Nele Merret Hollmann
    3. Tobias Melzer
    4. Panagiotis Katikaridis
    5. Lena Meins
    6. Bernd Simon
    7. Dirk Flemming
    8. Irmgard Sinning
    9. Janosch Hennig
    10. Axel Mogk

    This article has been curated by 1 group:

    • Curated by eLife

      eLife assessment

      This important manuscript details the characterization of ClpL from L. monocytogenes as an effective and autonomous AAA+ disaggregase that provides enhanced heat resistance to this food-borne pathogen. Supported by compelling evidence, the authors demonstrate that ClpL has DnaK-independent disaggregase activity towards a variety of aggregated model substrates, which is more potent than that observed with the endogenous canonical DnaK/ClpB bi-chaperone system. The work will be of broad interest to microbiologists and biochemists.

    Reviewed by eLife