eLife

Latest preprint reviews

1. Mapping variation in the morphological landscape of human cells with optical pooled CRISPRi screening

   This article has 8 authors:

   1. Ramon Lorenzo D. Labitigan
   2. Adrian L. Sanborn
   3. Cynthia V. Hao
   4. Caleb K. Chan
   5. Nathan M. Belliveau
   6. Eva M. Brown
   7. Mansi Mehrotra
   8. Julie A. Theriot

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     In this important study by Theriot et al., the authors utilize an impressive set of innovative approaches to conduct a CRISPRi pooled screen in human cells using large-scale microscopy screen data. They leverage an improved barcoding approach to identify genes targeted in specific cells and examine the effects on cell morphology using high-dimensional phenotypic analysis. The method and data presented are compelling.

   Reviewed by eLife

2. Metabolite profiling of human renal cell carcinoma reveals tissue-origin dominance in nutrient availability

   This article has 21 authors:

   1. Keene L Abbott
   2. Ahmed Ali
   3. Bradley I Reinfeld
   4. Amy Deik
   5. Sonu Subudhi
   6. Madelyn D Landis
   7. Rachel A Hongo
   8. Kirsten L Young
   9. Tenzin Kunchok
   10. Christopher S Nabel
   11. Kayla D Crowder
   12. Johnathan R Kent
   13. Maria Lucia L Madariaga
   14. Rakesh K Jain
   15. Kathryn E Beckermann
   16. Caroline A Lewis
   17. Clary B Clish
   18. Alexander Muir
   19. W Kimryn Rathmell
   20. Jeffrey Rathmell
   21. Matthew G Vander Heiden

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study provides an important finding that the local abundance of metabolites impacts the biology of the tumor microenvironment by utilizing kidney tumors from patients and adjacent normal tissues. The evidence supporting the claims of the authors is convincing. The work will of interest to the research community working on metabolism and kidney cancer especially.

   Reviewed by eLife

3. Visuo-motor updating in individuals with heightened autistic traits

   This article has 2 authors:

   1. Antonella Pomè
   2. Eckart Zimmermann

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study shows that a high autism quotient in neurotypical adults is associated with suboptimal motor planning and visual updating after eye movements, suggesting a disrupted efference copy mechanism. The implication is that abnormal visuomotor updating may contribute to sensory overload - a key symptom in autism spectrum disorder. The evidence presented is convincing, with few limitations, and should be of broad interest to neuroscientists at large.

   Reviewed by eLife

4. Knockdown of PHOX2B in the retrotrapezoid nucleus reduces the central CO2 chemoreflex in rats

   This article has 4 authors:

   1. Silvia Cardani
   2. Tara A Janes
   3. William Betzner
   4. Silvia Pagliardini

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This important study utilizes a viral-mediated short hairpin RNA (shRNA) approach to investigate in a novel way the role of the wild-type PHOX2B transcription factor expressed in critical chemosensory neurons in the brainstem retrotrapezoid nucleus (RTN) region for maintaining normal CO2 chemoreflex control of breathing in adult rats. The convincing results show blunted ventilation during elevated inhaled CO2 (hypercapnia) with knockdown of PHOX2B, accompanied by a reduced expression of Gpr4 and Task2 mRNA for the proposed RTN neuron proton sensor proteins GPR4 and TASK2. These results indicate that maintained expression of wild-type PHOX2B affects respiratory control in adult animals, complementing previous studies showing that PHOX2B-expressing RTN neurons may be critical for chemosensory control throughout the lifespan, and with implications for neurological disorders involving the RTN, which will be of interest to neuroscientists studying respiratory neurobiology as well as the neurodevelopmental control of motor behavior.

   Reviewed by eLife

5. Allosteric modulation of the CXCR4:CXCL12 axis by targeting receptor nanoclustering via the TMV-TMVI domain

   This article has 25 authors:

   1. Eva M García-Cuesta
   2. Pablo Martínez
   3. Karthik Selvaraju
   4. Gabriel Ulltjärn
   5. Adrián Miguel Gómez Pozo
   6. Gianluca D'Agostino
   7. Sofia Gardeta
   8. Adriana Quijada-Freire
   9. Patricia Blanco Gabella
   10. Carlos Roca
   11. Daniel del Hoyo
   12. Rodrigo Jiménez-Saiz
   13. Alfonso García-Rubia
   14. Blanca Soler Palacios
   15. Pilar Lucas
   16. Rosa Ayala-Bueno
   17. Noelia Santander Acerete
   18. Yolanda Carrasco
   19. Carlos Oscar Sorzano
   20. Ana Martinez
   21. Nuria E Campillo
   22. Lasse D Jensen
   23. Jose Miguel Rodriguez Frade
   24. César Santiago
   25. Mario Mellado

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This is an important study that describes an elegant modelling driven approach to design of allosteric antagonists for CXCR4 that have a selective effect on receptor nanocluster formation, cell polarisation and chemotaxis, but spare binding of CXCL12 to the receptor and inhibition of adenylate cyclase. This enables selective targeting of processes dependent upon cell polarisation and chemotaxis without impacting signalling effects and may avoid some of the toxicity associated with antagonists that target CXCL12 binding and thus block all CXCR4 signalling. The revised manuscript offers convincing evidence to support the claims. The modelling work is better described and additional data has been presented that better illustrates the unique features of the new antagonist. The in vivo studies in the zebrafish model open a path to studies in mammalian models.

   Reviewed by eLife

6. A Myelin Map of Trunk Folds in the Elephant Trigeminal Nucleus

   This article has 11 authors:

   1. Noémie Reveyaz
   2. Undine Schneeweiß
   3. Olivia Heise
   4. Ben Gerhardt
   5. Andreea M Gui
   6. Lena V Kaufmann
   7. Jette Alfken
   8. Jakob Reichmann
   9. Tim Salditt
   10. Thomas Hildebrandt
   11. Michael Brecht

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This valuable study uses neuroanatomical techniques to investigate somatosensory projections from the elephant trunk to the brainstem. Given its unique specializations, understanding how the elephant trunk is represented within the brain is of general interest to evolutionary and comparative neuroscientists. The authors present solid evidence for the existence of a novel isomorphism in which the folds of the trunk are mapped onto the trigeminal nucleus; however, due to their unusual structure, some uncertainty remains about the identification and anatomical organization of nuclei within the elephant brainstem.

   Reviewed by eLife

7. Antigenic drift and subtype interference shape A(H3N2) epidemic dynamics in the United States

   This article has 26 authors:

   1. Amanda C Perofsky
   2. John Huddleston
   3. Chelsea L Hansen
   4. John R Barnes
   5. Thomas Rowe
   6. Xiyan Xu
   7. Rebecca Kondor
   8. David E Wentworth
   9. Nicola Lewis
   10. Lynne Whittaker
   11. Burcu Ermetal
   12. Ruth Harvey
   13. Monica Galiano
   14. Rodney Stuart Daniels
   15. John W McCauley
   16. Seiichiro Fujisaki
   17. Kazuya Nakamura
   18. Noriko Kishida
   19. Shinji Watanabe
   20. Hideki Hasegawa
   21. Sheena G Sullivan
   22. Ian G Barr
   23. Kanta Subbarao
   24. Florian Krammer
   25. Trevor Bedford
   26. Cécile Viboud

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This paper explores the relationships among evolutionary and epidemiological quantities in influenza, and presents fundamental findings that substantially advance our understanding of the drivers of influenza epidemics. The authors use a rich set of data sources to gather and analyze compelling evidence on the roles of genetic distance, other influenza dynamics and epidemiological indicators in predicting influenza epidemics. The central findings highlight the significant influence of genetic distance on A(H3N2) virus epidemiology and emphasize the role of A(H1N1) virus incidence in shaping A(H3N2) epidemics, suggesting subtype interference as a key factor. This paper also makes relevant data available to the research community.

   Reviewed by eLife

8. Overnight fasting facilitates safety learning by changing the neurophysiological response to relief from threat omission

   This article has 4 authors:

   1. Silvia Papalini
   2. Tom Beckers
   3. Lukas Van Oudenhove
   4. Bram Vervliet

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     This study on the effects of fasting on safety learning rests on basic premises and concepts that both reviewers found difficult to follow. If these can be clarified, the findings may well be useful and of some utility for the field of emotional learning as well as experimental clinical psychology. However, the main claims of the study are only partially supported and are therefore incomplete.

   Reviewed by eLife

9. Novel risk loci for COVID-19 hospitalization among admixed American populations

   This article has 122 authors:

   1. Silvia Diz-de Almeida
   2. Raquel Cruz
   3. Andre D Luchessi
   4. José M Lorenzo-Salazar
   5. Miguel López de Heredia
   6. Inés Quintela
   7. Rafaela González-Montelongo
   8. Vivian Nogueira Silbiger
   9. Marta Sevilla Porras
   10. Jair Antonio Tenorio Castaño
   11. Julian Nevado
   12. Jose María Aguado
   13. Carlos Aguilar
   14. Sergio Aguilera-Albesa
   15. Virginia Almadana
   16. Berta Almoguera
   17. Nuria Alvarez
   18. Álvaro Andreu-Bernabeu
   19. Eunate Arana-Arri
   20. Celso Arango
   21. María J Arranz
   22. Maria-Jesus Artiga
   23. Raúl C Baptista-Rosas
   24. María Barreda- Sánchez
   25. Moncef Belhassen-Garcia
   26. Joao F Bezerra
   27. Marcos AC Bezerra
   28. Lucía Boix-Palop
   29. María Brion
   30. Ramón Brugada
   31. Matilde Bustos
   32. Enrique J Calderón
   33. Cristina Carbonell
   34. Luis Castano
   35. Jose E Castelao
   36. Rosa Conde-Vicente
   37. M Lourdes Cordero-Lorenzana
   38. Jose L Cortes-Sanchez
   39. Marta Corton
   40. M Teresa Darnaude
   41. Alba De Martino-Rodríguez
   42. Victor del Campo-Pérez
   43. Aranzazu Diaz de Bustamante
   44. Elena Domínguez-Garrido
   45. Rocío Eirós
   46. María Carmen Fariñas
   47. María J Fernandez-Nestosa
   48. Uxía Fernández-Robelo
   49. Amanda Fernández-Rodríguez
   50. Tania Fernández-Villa
   51. Manuela Gago-Dominguez
   52. Belén Gil-Fournier
   53. Javier Gómez-Arrue
   54. Beatriz González Álvarez
   55. Fernan Gonzalez Bernaldo de Quirós
   56. Anna González-Neira
   57. Javier González-Peñas
   58. Juan F Gutiérrez-Bautista
   59. María José Herrero
   60. Antonio Herrero-Gonzalez
   61. María A Jimenez-Sousa
   62. María Claudia Lattig
   63. Anabel Liger Borja
   64. Rosario Lopez-Rodriguez
   65. Esther Mancebo
   66. Caridad Martín-López
   67. Vicente Martín
   68. Oscar Martinez-Nieto
   69. Iciar Martinez-Lopez
   70. Michel F Martinez-Resendez
   71. Angel Martinez-Perez
   72. Juliana F Mazzeu
   73. Eleuterio Merayo Macías
   74. Pablo Minguez
   75. Victor Moreno Cuerda
   76. Silviene F Oliveira
   77. Eva Ortega-Paino
   78. Mara Parellada
   79. Estela Paz-Artal
   80. Ney PC Santos
   81. Patricia Pérez-Matute
   82. Patricia Perez
   83. M Elena Pérez-Tomás
   84. Teresa Perucho
   85. Mellina Pinsach-Abuin
   86. Guillermo Pita
   87. Ericka N Pompa-Mera
   88. Gloria L Porras-Hurtado
   89. Aurora Pujol
   90. Soraya Ramiro León
   91. Salvador Resino
   92. Marianne R Fernandes
   93. Emilio Rodríguez-Ruiz
   94. Fernando Rodriguez-Artalejo
   95. José A Rodriguez-Garcia
   96. Francisco Ruiz-Cabello
   97. Javier Ruiz-Hornillos
   98. Pablo Ryan
   99. José Manuel Soria
   100. Juan Carlos Souto
   101. Eduardo Tamayo
   102. Alvaro Tamayo-Velasco
   103. Juan Carlos Taracido-Fernandez
   104. Alejandro Teper
   105. Lilian Torres-Tobar
   106. Miguel Urioste
   107. Juan Valencia-Ramos
   108. Zuleima Yáñez
   109. Ruth Zarate
   110. Itziar de Rojas
   111. Agustín Ruiz
   112. Pascual Sánchez
   113. Luis Miguel Real
   114. SCOURGE Cohort Group
   115. Encarna Guillen-Navarro
   116. Carmen Ayuso
   117. Esteban Parra
   118. José A Riancho
   119. Augusto Rojas-Martinez
   120. Carlos Flores
   121. Pablo Lapunzina
   122. Ángel Carracedo

   This article has been curated by 1 group:

   • Curated by eLife

     eLife assessment

     The authors conduct a valuable GWAS meta-analysis for COVID-19 hospitalization in admixed American populations and prioritized risk variants and genes. The evidence supporting the claims of the authors is solid. The work will be of interest to scientists studying the genetic basis of COVID pathogenesis.

   Reviewed by eLife

10. Detecting, mapping, and suppressing the spread of a decade-long Pseudomonas aeruginosa nosocomial outbreak with genomics

    This article has 24 authors:

    1. William Stribling
    2. Lindsey R Hall
    3. Aubrey Powell
    4. Casey Harless
    5. Melissa J Martin
    6. Brendan W Corey
    7. Erik Snesrud
    8. Ana Ong
    9. Rosslyn Maybank
    10. Jason Stam
    11. Katelyn V Bartlett
    12. Brendan T Jones
    13. Lan N Preston
    14. Katherine F Lane
    15. Bernadette Thompson
    16. Lynn M Young
    17. Yoon I Kwak
    18. Alice E Barsoumian
    19. Ana Elizabeth Markelz
    20. John L Kiley
    21. Robert J Cybulski
    22. Jason W Bennett
    23. Patrick T Mc Gann
    24. Francois Lebreton

    This article has been curated by 1 group:

    • Curated by eLife

      eLife Assessment

      This important work presents an example of how genomic data can be used to improve understanding of an ongoing, long-term bacterial outbreak in a hospital with an application to multi-drug resistant Pseudomonas aeruginosa, and will be of interest to researchers concerned with the spread of drug-resistant bacteria in hospital settings. The convincing genomic analyses highlight the value of routine surveillance of patients and environmental sampling and show how such data can help in dating the origin of the outbreak and in characterising the epidemic lineages. These findings highlight the importance of understanding environmental factors contributing to the transmission of P. aeruginosa for guiding and tailoring infection control efforts.

    Reviewed by eLife