Systems Biology

Evolution of cell size control is canalized towards adders or sizers by cell cycle structure and selective pressures

1. Felix Proulx-Giraldeau
2. Jan M Skotheim
3. Paul François

• Curated by eLife

  Evaluation Summary:

  This paper develops evolutionary simulations to identify the type of molecular networks that can give rise to size control. We now know a lot about the functional consequences and underlying molecular biology of different cell size control strategies, but comparatively less about which factors select for particular mechanisms. The authors address this point in an evolutionary framework. They show that the evolution of a specific cell size control mechanism is dependent on the cell cycle structure. The paper will interest researchers in development, evolution, and physics of biological systems.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Multi‐omic network analysis identified betacellulin as a novel target of omega‐3 fatty acid attenuation of western diet‐induced nonalcoholic steatohepatitis

1. Jyothi Padiadpu
2. Manuel Garcia‐Jaramillo
3. Nolan K Newman
4. Jacob W Pederson
5. Richard Rodrigues
6. Zhipeng Li
7. Sehajvir Singh
8. Philip Monnier
9. Giorgio Trinchieri
10. Kevin Brown
11. Amiran K Dzutsev
12. Natalia Shulzhenko
13. Donald B Jump
14. Andrey Morgun

Reviewed by Review Commons

A hierarchical model for external electrical control of an insect, accounting for inter-individual variation of muscle force properties

1. Dai Owaki
2. Volker Dürr
3. Josef Schmitz

• Curated by eLife

  eLife assessment

  This valuable work presents new results to characterize the relationship between electrical excitation and torque generation in stick insect joints. The evidence supporting this work is a series of torque-voltage measurements across individuals. The strength of evidence is solid in supporting the outcomes, but some details of the methodology, which could potentially shed light on the sources of this variation, are missing.

Reviewed by eLife

Deep proteome profiling reveals signatures of age and sex differences in paw skin and sciatic nerve of naïve mice

1. Feng Xian
2. Julia Regina Sondermann
3. David Gomez Varela
4. Manuela Schmidt

• Curated by eLife

  Evaluation Summary:

  In addition to providing extensive proteomics profiling datasets. this manuscript is fundamental that sheds light on the importance of appropriate experimental design for mouse disease model which have been overlooked so far. The results look quite solid based on the proper methodology. This type of work is extremely valuable to many biomedical scientists in the field for conducting reproducible research especially in the preclinical studies and properly interpreting the results.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Resource allocation accounts for the large variability of rate-yield phenotypes across bacterial strains

1. Valentina Baldazzi
2. Delphine Ropers
3. Jean-Luc Gouzé
4. Tomas Gedeon
5. Hidde de Jong

• Curated by eLife

  Evaluation Summary:

  This study develops a resource allocation model for E. coli growing under steady-state conditions. The model describes both growth rate and yield and has been subjected to validation by comparison with a compiled data set. The manuscript addresses an important problem of interest to a wide range of investigators. At the same time, the authors would need to explore different assumptions for the housekeeping proteome fraction (phi_q) to ensure the model is robust.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Quantifying how post-transcriptional noise and gene copy number variation bias transcriptional parameter inference from mRNA distributions

1. Xiaoming Fu
2. Heta P Patel
3. Stefano Coppola
4. Libin Xu
5. Zhixing Cao
6. Tineke L Lenstra
7. Ramon Grima

Reviewed by Review Commons

Decoding mechanism of action and sensitivity to drug candidates from integrated transcriptome and chromatin state

1. Caterina Carraro
2. Lorenzo Bonaguro
3. Jonas Schulte-Schrepping
4. Arik Horne
5. Marie Oestreich
6. Stefanie Warnat-Herresthal
7. Tim Helbing
8. Michele De Franco
9. Kristian Haendler
10. Sach Mukherjee
11. Thomas Ulas
12. Valentina Gandin
13. Richard Goettlich
14. Anna C Aschenbrenner
15. Joachim L Schultze
16. Barbara Gatto

• Curated by eLife

  Evaluation Summary:

  This article applies a systems biology approach to understand the mechanism of action of 3-chloropiperidines (a class of anticancer drugs) in cancer cells and evaluate their sensitivity to drugs. It integrates transcriptomic and open-chromatin data and utilizes sound statistical frameworks for building a sensitivity model. The author's methodology can be applied to early-stage drug discovery. This paper will be of interest to the large class of people who tried to understand how omics data will help drug discovery. It sets a new framework to integrate transcriptome and chromatin accessibility data to identify the key mechanisms of action and provide potential disease targets, which will help speed up the early phases of drug discovery.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. All three reviewers agreed to share their names with the authors.)

Reviewed by eLife

Concentration fluctuations in growing and dividing cells: Insights into the emergence of concentration homeostasis

1. Chen Jia
2. Abhyudai Singh
3. Ramon Grima

Reviewed by Review Commons

On the flexibility of the cellular amination network in E coli

1. Helena Schulz-Mirbach
2. Alexandra Müller
3. Tong Wu
4. Pascal Pfister
5. Selçuk Aslan
6. Lennart Schada von Borzyskowski
7. Tobias J Erb
8. Arren Bar-Even
9. Steffen N Lindner

• Curated by eLife

  Evaluation Summary:

  In this paper, the authors demonstrate that a reversible amination network that allows nitrogen transfer via transaminases for synthesis of several amino acids can be constructed in laboratory strains through clever and carefully designed experiments. As a result, this work should be of interest to microbiologists, biochemists, synthetic biologists, and biotechnologists.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Information content differentiates enhancers from silencers in mouse photoreceptors

1. Ryan Z Friedman
2. David M Granas
3. Connie A Myers
4. Joseph C Corbo
5. Barak A Cohen
6. Michael A White

• Curated by eLife

  Evaluation Summary:

  This manuscript will be of interest to geneticists seeking to establish rules that govern gene regulation. To explain why a sequence enhances, rather than silences, gene transcription the authors draw our attention away from the binding of a single transcription factor, to focus instead on the number and diversity of transcription factor molecules that bind to it. Using a relatively simple metric called sequence information content they appear to be able to improve the prediction of enhancer over silencer sequences. A concern is whether the silencers are true silencers, or whether they only act as such in this specific experimental paradigm.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife