Plant Biology

Subcellular visualization of the distribution of atmospheric dinitrogen fixed by Gluconacetobacter diazotrophicus bacteria in maize

1. Greg McMahon
2. Stephanie Rey
3. Katie Moore
4. Gina Greenidge
5. Dhaval Patel
6. Erik H Murchie
7. David Dent
8. Edward Cocking

• Curated by eLife

  eLife assessment

  This solid and innovative study explores the uptake of fixed nitrogen in maize chloroplasts facilitated by symbiotic Gluconacetobacter diazotrophicus bacteria. The findings provide valuable insights into plant-microbe interactions, particularly highlighting a symbiotic mechanism of nitrogen delivery independent nodule formation. Additional controls would help to substantiate the findings and enhance the overall strength of the evidence.

Reviewed by eLife

An array of Zymoseptoria tritici effectors suppress plant immune responses

1. E. Thynne
2. H. Ali
3. K. Seong
4. M. Abukhalaf
5. M. A. Guerreiro
6. V. M. Flores-Nunez
7. R. Hansen
8. A. Bergues
9. M. J. Salman
10. J. J. Rudd
11. K. Kanyuka
12. A. Tholey
13. K. V. Krasileva
14. G. J. Kettles
15. E. H. Stukenbrock

Reviewed by PREreview

Xanthomonas citri subsp. citri type III effector PthA4 directs the dynamical expression of a putative citrus carbohydrate-binding protein gene for canker formation

1. Xinyu Chen
2. Huasong Zou
3. Tao Zhuo
4. Wei Rou
5. Wei Wu
6. Xiaojing Fan

• Curated by eLife

  eLife assessment

  This valuable study provides new insight into potential subtle dynamics in effector biology. The data presented generally support the claims, but in some cases, significant controls are missing and so the overall work is currently incomplete. If the limitations can be addressed, this work should be of broad relevance for biologists interested in molecular plant-microbe interactions.

Reviewed by eLife

Hyperspectral imaging for chloroplast movement detection

1. Paweł Hermanowicz
2. Justyna Łabuz

Reviewed by Arcadia Science

Polarised subcellular activation of ROPs by specific ROPGEFs drives pollen germination in Arabidopsis thaliana

1. Alida Melissa Bouatta
2. Andrea Lepper
3. Philipp Denninger

Reviewed by Review Commons

Long non-coding RNAs regulate the expression of cell surface receptors in plants

1. Hemal Bhasin
2. Hasna Khan
3. Zachary Kileeg
4. G. Adam Mott

Reviewed by Review Commons

Using AlphaFold Multimer to discover interkingdom protein-protein interactions

1. Felix Homma
2. Joy Lyu
3. Renier A. L. van der Hoorn

Reviewed by PREreview

Plasmodesmal closure elicits stress responses

1. Estee E. Tee
2. Andrew Breakspear
3. Diana Papp
4. Hannah R. Thomas
5. Catherine Walker
6. Annalisa Bellandi
7. Christine Faulkner

Reviewed by preLights

Arabidopsis transcriptome responses to low water potential using high-throughput plate assays

1. Stephen Gonzalez
2. Joseph Swift
3. Adi Yaaran
4. Jiaying Xu
5. Charlotte Miller
6. Natanella Illouz-Eliaz
7. Joseph R Nery
8. Wolfgang Busch
9. Yotam Zait
10. Joseph R Ecker

• Curated by eLife

  eLife assessment

  This work critically evaluates several widely-used assays of transcriptional responses to water limitation in Arabidopsis grown on defined agar-solidified media and, finding inconsistent responses in root transcriptome responses, introduces a new 'hard agar' assay with more consistent responses. The work is valuable as a simple and alternative experimental system that would enable high-throughput genetic screening (and GWAS) to assess the impacts of environmental perturbations on transcriptional responses in various genetic backgrounds. Within this scope, the work is solid, though the debate about whether field-level physiological inferences can be made from such assays remains.

Reviewed by eLife

Two NLR immune receptors acquired high-affinity binding to a fungal effector through convergent evolution of their integrated domain

1. Aleksandra Białas
2. Thorsten Langner
3. Adeline Harant
4. Mauricio P Contreras
5. Clare EM Stevenson
6. David M Lawson
7. Jan Sklenar
8. Ronny Kellner
9. Matthew J Moscou
10. Ryohei Terauchi
11. Mark J Banfield
12. Sophien Kamoun

• Curated by eLife

  Evaluation Summary:

  Convergent evolution is often observed in nature, but the molecular mechanisms allowing similar functions to independently emerge are rarely understood. This work determines how the high-affinity recognition of a pathogenic effector produced by the rice blast fungus, Avr-PikD, evolved in the immune receptor Pik-1. The integration of molecular evolution analyses with structure-function biochemical testing is novel to the field and the data quality is exceptional. In addition to advancing knowledge of host-microbe co-evolution, this work is exemplary in its transparency and the breadth of approaches utilized to understand protein evolution, and we expect that this study will provide a conceptual framework for similar studies in the future.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife, PREreview