Physiology

TRPV1 drugs alter core body temperature via central projections of primary afferent sensory neurons

1. Wendy Wing Sze Yue
2. Lin Yuan
3. Joao M Braz
4. Allan I Basbaum
5. David Julius

• Curated by eLife

  Evaluation Summary:

  The manuscript by Yue et al describes studies investigating the role of sensory neuron versus arteriole expression of Trpv1 in body temperature control. This is a detail about the contribution of different cells which has significance because of the reported on-target side-effect of hyperthermia by Trpv1-antagonists. The study shows that the effects on body temperature are predominantly produced through sensory neurons. From these studies it is speculated that the actions of Trpv1 might be pharmacologically modified to permit dissociation of the effects on neurogenic inflammation and the undesirable effects on body temperature.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. All reviewers agreed to share their names with the authors.)

Reviewed by eLife

Insulin sensitivity is preserved in mice made obese by feeding a high starch diet

1. Amanda E Brandon
2. Lewin Small
3. Tuong-Vi Nguyen
4. Eurwin Suryana
5. Henry Gong
6. Christian Yassmin
7. Sarah E Hancock
8. Tamara Pulpitel
9. Sophie Stonehouse
10. Letisha Prescott
11. Melkam A Kebede
12. Belinda Yau
13. Lake-Ee Quek
14. Greg M Kowalski
15. Clinton R Bruce
16. Nigel Turner
17. Gregory J Cooney

• Curated by eLife

  Evaluation Summary:

  This study evaluates the effects of two distinct dietary methods that cause obesity in mice (high fat vs high starch) on insulin sensitivity and glucose homeostasis. Through a series of nicely performed physiology experiments, the authors demonstrated that high starch feeding causes obesity without deleterious effects on insulin sensitivity. This work will have an impact in the field and help define the important lipid mediators of metabolic disease.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Rewiring of liver diurnal transcriptome rhythms by triiodothyronine (T3) supplementation

1. Leonardo Vinicius Monteiro de Assis
2. Lisbeth Harder
3. José Thalles Lacerda
4. Rex Parsons
5. Meike Kaehler
6. Ingolf Cascorbi
7. Inga Nagel
8. Oliver Rawashdeh
9. Jens Mittag
10. Henrik Oster

• Curated by eLife

  Evaluation Summary:

  Monteiro de Assis et al. demonstrate a role for T3 in modulating circadian metabolic rhythms both systemically and within the liver. The findings extend the molecular framework in which organismal metabolism is coordinated in a circadian fashion.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

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Semaphorin3F reduces vascular endothelial and smooth muscle cell PI3K activation and decreases neointimal plaque formation

1. Chutima Rattanasopa
2. David Castano-Mayan
3. Chengxun Su
4. Aaron J. Farrugia
5. Maria Corlianò
6. Pakhwan Nilcham
7. Crystal Pang
8. Monalisa Hota
9. Koh Ser Mei
10. Wendy Lee
11. Dasan Mary Cibi
12. Atsu Aiba
13. Manvendra K. Singh
14. Siew Cheng Wong
15. Olaf Rotzschke
16. Alexander Bershadsky
17. Han Wei Hou
18. Elisa A. Liehn
19. Sujoy Ghosh
20. Roshni R. Singaraja

• Curated by eLife

  Evaluation Summary:

  The authors provide novel evidence that semaphorin signaling (SEMA3F) is engaged in the vascular endothelium and smooth muscle to confer atheroprotection. They show that SEMA3F reduces the activity of key enzyme Phosphoinositide 3-kinase to decrease smooth muscle cell proliferation, migration, and phenotype switching, which contributes to atheroprotection. The study has significant translational potential and yields a new therapeutic target.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Connexin hemichannels with prostaglandin release in anabolic function of bone to mechanical loading

1. Dezhi Zhao
2. Manuel A Riquelme
3. Teja Guda
4. Chao Tu
5. Huiyun Xu
6. Sumin Gu
7. Jean X Jiang

• Curated by eLife

  Evaluation Summary:

  The authors have used two transgenic mouse models expressing dominant negative Cx43 mutants to evaluate the role of Cx43 hemichannels in mechanical loading response in bone. While understanding the molecular mechanisms by which osteocytes respond to mechanical strain is of interest in the skeletal biology arena, the conclusions of this study are not fully supported by experimental data and are of only incremental in nature.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript.The reviewers remained anonymous to the authors.)

Reviewed by eLife

Septin7 is indispensable for proper skeletal muscle architecture and function

1. Mónika Gönczi
2. Zsolt Ráduly
3. László Szabó
4. János Fodor
5. Andrea Telek
6. Nóra Dobrosi
7. Norbert Balogh
8. Péter Szentesi
9. Gréta Kis
10. Miklós Antal
11. György Trencsenyi
12. Beatrix Dienes
13. László Csernoch

• Curated by eLife

  Evaluation Summary:

  The study of previously unexplored roles of a cytoskeleton protein termed Septin-7 in muscle could be a significant contribution to understand muscle development and regeneration. The majority of the data support the importance of Septin-7 protein in muscle physiology. With some additional experiments and data analysis to strengthen the mechanistic characterization of Septin-7 in muscle physiology, this manuscript will be of broad interest to readers in the skeletal muscle research field.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Enteroendocrine cell types that drive food reward and aversion

1. Ling Bai
2. Nilla Sivakumar
3. Shenliang Yu
4. Sheyda Mesgarzadeh
5. Tom Ding
6. Truong Ly
7. Timothy V Corpuz
8. James CR Grove
9. Brooke C Jarvie
10. Zachary A Knight

• Curated by eLife

  Evaluation Summary:

  The effective approach for targeting select subsets of enteroendocrine cells described here will be important for neuroscientists, endocrinologists, microbiologists, and other scientists studying nutritional biology. The study reveals new detail of how enteroendocrine cells signal through spinal and vagal sensory neurons to control immediate behavior and to guide learning about potential food sources. Overall, the approach is well thought out and the results are interesting and unexpected.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Gene and protein expression and metabolic flux analysis reveals metabolic scaling in liver ex vivo and in vivo

1. Ngozi D Akingbesote
2. Brooks P Leitner
3. Daniel G Jovin
4. Reina Desrouleaux
5. Dennis Owusu
6. Wanling Zhu
7. Zongyu Li
8. Michael N Pollak
9. Rachel J Perry

• Curated by eLife

  Evaluation Summary:

  The study provides evidence that specific transcriptional responses may underpin the observation that metabolic rates often scale inversely with body mass. The conclusions are supported by direct measurement of metabolic fluxes in mouse and rat livers, although generalizations to other settings remain to be rigorously tested. The study has broad implications for researching and studying animal metabolism and physiology.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Molecular programming modulates hepatic lipid metabolism and adult metabolic risk in the offspring of obese mothers in a sex-specific manner

1. Christina Savva
2. Luisa A. Helguero
3. Marcela González-Granillo
4. Tânia Melo
5. Daniela Couto
6. Bo Angelin
7. Maria Rosário Domingues
8. Xidan Li
9. Claudia Kutter
10. Marion Korach-André

Reviewed by Review Commons

Residual force enhancement is affected more by quadriceps muscle length than stretch amplitude

1. Patrick Bakenecker
2. Tobias Weingarten
3. Daniel Hahn
4. Brent Raiteri

• Curated by eLife

  Evaluation Summary:

  The authors have systematically examined relationships between muscle length and force potentiation in young adults using very carefully conducted and controlled measurements by dynamometry and estimated using patellar tendon shear wave speed. The paper should be of interest to those who study human performance.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife