Pharmacology and Toxicology

Single-cell transcriptomics identifies a dampened neutrophil function and accentuated T-cell cytotoxicity in tobacco flavored e-cigarette exposed mouse lungs

1. Gagandeep Kaur
2. Thomas Lamb
3. Ariel Tjitropranoto
4. Irfan Rahman

• Curated by eLife

  eLife Assessment

  This manuscript by Kaur et al. identifies differential gene expression observed in distinct mouse lung cell populations, namely myeloid and lymphoid cells, upon short-term exposure to e-cig aerosols with various flavors. Their findings are potentially useful because the single-cell sequencing data provides a reference for future studies of genes and cellular pathways that are most affected by e-cig aerosols and their components. However, the evidence is incomplete due to limited statistical analyses and few biological replicates, as well as a lack of experimental validation.

Reviewed by eLife

A Novel Bispecific Antibody CVL006 Superior to AK112 for Dual Targeting of PD-L1 and VEGF in Cancer Therapy

1. Chunyan Wang
2. Hao Huang
3. Zeng Song
4. Zhongyuan Li
5. Jinwen Huang
6. Liang Cao
7. Ziai Wu
8. Junfang Pan
9. Xiaokun Shen

Reviewed by PREreview

Interleukin-4 induces CD11c+ microglia leading to amelioration of neuropathic pain in mice

1. Keita Kohno
2. Ryoji Shirasaka
3. Keita Hirose
4. Takahiro Masuda
5. Makoto Tsuda

• Curated by eLife

  eLife Assessment

  This study is important as it highlighted how IL-4 regulates the reactive state of a specific microglial population by increasing the proportion of CD11c+ microglial cells and ultimately suppressing neuropathic pain. It provided convincing evidence on the pain-resolving roles of microglia. However, the authors are encouraged to clarify data interpretation and integrate the study's findings into the existing knowledge about microglia, monocytes, and macrophages.

Reviewed by eLife

Target protein identification in live cells and organisms with a non-diffusive proximity tagging system

1. Yingjie Sun
2. Changheng Li
3. Xiaofei Deng
4. Wenjie Li
5. Xiaoyi Deng
6. Weiqi Ge
7. Miaoyuan Shi
8. Ying Guo
9. Yanxun V Yu
10. Hai-bing Zhou
11. Youngnam N Jin

• Curated by eLife

  eLife Assessment

  The study presents important findings that reveal SEPHS2 and VPS37C as new potential drug targets for dasatinib and hydroxychloroquine respectively in addition to confirming known targets of these drugs. The evidence provided is compelling as observed in the methods, data and analyses. This article will be of great interest to chemical biologists, biochemists, and scientists in drug discovery and diagnostics.

Reviewed by eLife

Visualization of endogenous G proteins on endosomes and other organelles

1. Wonjo Jang
2. Kanishka Senarath
3. Gavin Feinberg
4. Sumin Lu
5. Nevin A Lambert

• Curated by eLife

  eLife assessment

  This important study investigates the intracellular localization patterns of G proteins involved in GPCR signaling, presenting compelling evidence for their preference for plasma and lysosomal membranes over endosomal, endoplasmic reticulum, and Golgi membranes. This discovery has significant implications for understanding GPCR action and signaling from intracellular locations. This research will interest cell biologists studying protein trafficking and pharmacologists exploring localized signaling phenomena.

Reviewed by eLife

SntB triggers the antioxidant pathways to regulate development and aflatoxin biosynthesis in Aspergillus flavus

1. Dandan Wu
2. Chi Yang
3. Yanfang Yao
4. Dongmei Ma
5. Hong Lin
6. Ling Hao
7. Wenwen Xin
8. Kangfu Ye
9. Minghui Sun
10. Yule Hu
11. Yanling Yang
12. Zhenhong Zhuang

• Curated by eLife

  eLife assessment

  In this useful study, the authors investigate the regulatory mechanisms related to toxin production and pathogenicity in Aspergillus flavus. Their observations indicate that the SntB protein regulates morphogenesis, aflatoxin biosynthesis, and the oxidative stress response. The data supporting the conclusions are compelling and contribute significantly the advancing the understanding of SntB function.

Reviewed by eLife

Synthesis and biological assessment of chalcone and pyrazoline derivatives as novel inhibitor for ELF3-MED23 interaction

1. Soo-Yeon Hwang
2. Kyung-Hwa Jeon
3. Hwa-Jong Lee
4. Inhye Moon
5. Sehyun Jung
6. Seul-Ah Kim
7. Hyunji Jo
8. Seojeong Park
9. Misun Ahn
10. Soo-Yeon Kwak
11. Younghwa Na
12. Youngjoo Kwon

• Curated by eLife

  eLife assessment

  This valuable study characterized a new set of small molecules targeting the interaction between ELF3-MED23, with one of the reported compounds representing a promising novel therapeutic strategy, The evidence supporting the conclusions is convincing. This article will be of interest to medical and cell biologists working on cancer and, particularly, on HER2-overexpression cancers.

Reviewed by eLife

Noncaloric monosaccharides induce excessive sprouting angiogenesis in zebrafish via foxo1a-marcksl1a signal

1. Xiaoning Wang
2. Jinxiang Zhao
3. Jiehuan Xu
4. Bowen Li
5. Xia Liu
6. Gangcai Xie
7. Xuchu Duan
8. Dong Liu

• Curated by eLife

  eLife assessment

  This valuable study investigates the effect of noncaloric monosaccharides, sugar substitutes that are commonly used by diabetic patients, on angiogenesis in the zebrafish embryo. The authors show that noncaloric monosaccharides and glucose similarly induce excessive blood vessel formation due to the increased formation of tip cells by endothelial cells through the foxo1a-marcksl1a pathway. This solid study is of interest for the medical community in charge of the prevention and of the treatment of diabetes and other metabolic diseases.

Reviewed by eLife

Trazodone, dibenzoylmethane and tauroursodeoxycholic acid do not prevent motor dysfunction and neurodegeneration in Marinesco-Sjögren syndrome mice

1. Giada Lavigna
2. Anna Grasso
3. Chiara Pasini
4. Valentina Grande
5. Laura Mignogna
6. Elena Restelli
7. Antonio Masone
8. Claudia Fracasso
9. Jacopo Lucchetti
10. Marco Gobbi
11. Roberto Chiesa

Reviewed by Review Commons

Nonspecific membrane bilayer perturbations by ivermectin underlie SARS-CoV-2 in vitro activity

1. Richard T Eastman
2. Radda Rusinova
3. Karl F Herold
4. Xi-Ping Huang
5. Patricia Dranchak
6. Ty C Voss
7. Sandeep Rana
8. Jonathan H Shrimp
9. Alex D White
10. Hugh C Hemmings
11. Bryan L Roth
12. James Inglese
13. Olaf S Andersen
14. Jayme L Dahlin

Reviewed by Rapid Reviews Infectious Diseases