Molecular Biology

Sialic acid and fucose residues on the SARS-CoV-2 receptor binding domain modulate IgG reactivity

1. Ebba Samuelsson
2. Ekaterina Mirgorodskaya
3. Kristina Nyström
4. Malin Bäckström
5. Jan-Åke Liljeqvist
6. Rickard Nordén

Reviewed by ScreenIT

High activity of an affinity-matured ACE2 decoy against Omicron SARS-CoV-2 and pre-emergent coronaviruses

1. Joshua J. Sims
2. Sharon Lian
3. Rosemary L. Meggersee
4. Aradhana Kasimsetty
5. James M. Wilson

Reviewed by ScreenIT

Application of sequence semantic and integrated cellular geography approach to study biogenesis of exonic circular RNA

1. Rajnish Kumar
2. Tapobrata Lahiri
3. Manoj Kumar Pal

Reviewed by PeerRef

Binary outcomes of enhancer activity underlie stable random monoallelic expression

1. Djem U Kissiov
2. Alexander Ethell
3. Sean Chen
4. Natalie K Wolf
5. Chenyu Zhang
6. Susanna M Dang
7. Yeara Jo
8. Katrine N Madsen
9. Ishan Paranjpe
10. Angus Y Lee
11. Bryan Chim
12. Stefan A Muljo
13. David H Raulet

• Curated by eLife

  Evaluation Summary:

  In this manuscript by Kissiov et al. the authors show that enhancers can play an instructive role in controlling stable random monoallelic expression (RME). In order to do so, they initially focus on a limited set of natural killer (NK) receptor genes that are subject to RME, which they investigate using several in vivo genetic models. Furthermore, they also show that RME can be considerably more prevalent than previously thought and that enhancer strength and/or number might influence the extent of RME for different genes. One remaining question may be whether this model may apply to other gene types than hematopoietic-related genes. Overall, this is a highly relevant manuscript with major implications in gene regulation and enhancer biology and, thus, of broad scientific interest.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

Rvb1/Rvb2 proteins couple transcription and translation during glucose starvation

1. Yang S Chen
2. Wanfu Hou
3. Sharon Tracy
4. Alex T Harvey
5. Vince Harjono
6. Fan Xu
7. James J Moresco
8. John R Yates
9. Brian M Zid

Reviewed by Review Commons

Protection of nascent DNA at stalled replication forks is mediated by phosphorylation of RIF1 intrinsically disordered region

1. Sandhya Balasubramanian
2. Matteo Andreani
3. Júlia Goncalves Andrade
4. Tannishtha Saha
5. Devakumar Sundaravinayagam
6. Javier Garzón
7. Wenzhu Zhang
8. Oliver Popp
9. Shin-ichiro Hiraga
10. Ali Rahjouei
11. Daniel B Rosen
12. Philipp Mertins
13. Brian T Chait
14. Anne D Donaldson
15. Michela Di Virgilio

• Curated by eLife

  Evaluation Summary:

  RIF1 is a protein of the DNA damage response with key roles on genome integrity: it prevents DSB resection and hence accurate HR repair, whilst protecting stalled forks from degradation under replication stress conditions. The authors' main finding is the identification of 3 residues in RIF1 protein, that can be phosphorylated in ATM/ATR-dependent manner. However, this phosphorylation is dispensable for the ability of RIF1 to limit double-strand break resection, but is required to counteract the degradation of stalled replication intermediates mediated by the DNA2 nuclease. Therefore, the manuscript suggests that the three sites can provide a potential switch between the two functions of RIF1. These findings will spark the interest of readers working in the DNA replication and repair fields. However, the actual mechanism by which blocking RIF1 phosphorylation prevents RIF1 function at replication forks still needs to be determined.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Sequence-assignment validation in cryo-EM models with checkMySequence

1. Grzegorz Chojnowski

Reviewed by ScreenIT

Clinical grade ACE2 effectively inhibits SARS-CoV-2 Omicron infections

1. Vanessa Monteil
2. Devignot Stephanie
3. Jonas Klingström
4. Charlotte Thålin
5. Max J. Kellner
6. Wanda Christ
7. Sebastian Havervall
8. Stefan Mereiter
9. Sylvia Knapp
10. Nuria Montserrat
11. Benedict Braunsfeld
12. Ivona Kozieradzki
13. Omar Hasan Ali
14. Astrid Hagelkruys
15. Johannes Stadlmann
16. Chris Oostenbrink
17. Gerald Wirnsberger
18. Josef M. Penninger
19. Ali Mirazimi

Reviewed by ScreenIT

Key benefits of dexamethasone and antibody treatment in COVID-19 hamster models revealed by single-cell transcriptomics

1. Emanuel Wyler
2. Julia M. Adler
3. Kathrin Eschke
4. G. Teixeira Alves
5. Stefan Peidli
6. Fabian Pott
7. Julia Kazmierski
8. Laura Michalick
9. Olivia Kershaw
10. Judith Bushe
11. Sandro Andreotti
12. Peter Pennitz
13. Azza Abdelgawad
14. Dylan Postmus
15. Christine Goffinet
16. Jakob Kreye
17. S Momsen Reincke
18. Harald Prüss
19. Nils Blüthgen
20. Achim D. Gruber
21. Wolfgang M. Kuebler
22. Martin Witzenrath
23. Markus Landthaler
24. Geraldine Nouailles
25. Jakob Trimpert

Reviewed by ScreenIT

Transcriptomic responses of the human kidney to acute injury at single cell resolution

1. Christian Hinze
2. Christine Kocks
3. Janna Leiz
4. Nikos Karaiskos
5. Anastasiya Boltengagen
6. Christopher Mark Skopnik
7. Jan Klocke
8. Jan-Hendrik Hardenberg
9. Helena Stockmann
10. Inka Gotthardt
11. Benedikt Obermayer
12. Laleh Haghverdi
13. Emanuel Wyler
14. Markus Landthaler
15. Sebastian Bachmann
16. Andreas C. Hocke
17. Victor Corman
18. Jonas Busch
19. Wolfgang Schneider
20. Nina Himmerkus
21. Markus Bleich
22. Kai-Uwe Eckardt
23. Philipp Enghard
24. Nikolaus Rajewsky
25. Kai M. Schmidt-Ott

Reviewed by ScreenIT