Molecular Biology

Phosphorylation of Rab29 at Ser185 regulates its localization and role in the lysosomal stress response in concert with LRRK2

1. Tadayuki Komori
2. Tomoki Kuwahara
3. Tetta Fujimoto
4. Maria Sakurai
5. Ikuko Koyama-Honda
6. Mitsunori Fukuda
7. Takeshi Iwatsubo

Reviewed by Review Commons

STAT3 promotes RNA polymerase III-directed transcription by controlling the miR-106a-5p/TP73 axis

1. Cheng Zhang
2. Shasha Zhao
3. Huan Deng
4. Shihua Zhang
5. Juan Wang
6. Xiaoye Song
7. Deen Yu
8. Yue Zhang
9. Wensheng Deng

• Curated by eLife

  eLife assessment

  The author arrive at the convincing conclusion that STAT3 expression promotes TFIIIB assembly through miR-106A-5p-mediated inhibition of TP73 expression, thereby increasing Pol III transcription, which contributes to enhanced cell proliferation. The data are very good and clearly support the proposed model.

Reviewed by eLife

Myoglobin-derived iron causes wound enlargement and impaired regeneration in pressure injuries of muscle

1. Nurul Jannah Mohamed Nasir
2. Hans Heemskerk
3. Julia Jenkins
4. Nur Hidayah Hamadee
5. Ralph Bunte
6. Lisa Tucker-Kellogg

Reviewed by Review Commons

eCRUIS captures RNA-protein interaction in vitro and in vivo

1. Ziheng Zhang
2. Yuanbing Zhang
3. Ji-Long Liu

Reviewed by Arcadia Science

Recognition of galactose by a scaffold protein recruits a transcriptional activator for the GAL regulon induction in Candida albicans

1. Xun Sun
2. Jing Yu
3. Cheng Zhu
4. Xinreng Mo
5. Qiangqiang Sun
6. Dandan Yang
7. Chang Su
8. Yang Lu

• Curated by eLife

  eLife assessment

  This important manuscript investigates the circuitry connecting the galactose utilization regulon of the human pathogen and model organism Candida albicans to the sensing of galactose. In the non-pathogenic model yeast Saccharomyces cerevisiae this circuit represents a textbook model that rivals the lac operon as a teaching tool. Using a broad array of mainly classical approaches, this study convincingly demonstrates the transcriptional activators that are required for galactose (and GlcNAc) responsive galactose metabolic genes in C. albicans. The recognition of just how different the regulation of the galactose pathway across fungal species represents an important advance in our understanding of the evolution of the regulatory control of these circuits, and would make a nice addition to the textbook version of eukaryotic gene regulation.

Reviewed by eLife

Hypoxia truncates and constitutively activates the key cholesterol synthesis enzyme squalene monooxygenase

1. Hudson W Coates
2. Isabelle M Capell-Hattam
3. Ellen M Olzomer
4. Ximing Du
5. Rhonda Farrell
6. Hongyuan Yang
7. Frances L Byrne
8. Andrew J Brown

• Curated by eLife

  eLife assessment

  Hudson and colleagues provide a new link between oxygen sensing and cholesterol synthesis. In previous studies, this group had shown that the cholesterol synthetic enzyme squalene monooxygenase (SM) is subjected to partial proteasomal degradation, which leads to the production of a truncated, constitutively active enzyme. Here, the authors provide evidence for the physiological significance of SM truncation by showing that subjecting cells to hypoxia (oxygen deprivation) induces truncation of SM. The synthesis of cholesterol requires 11 molecules of oxygen and SM is the first oxygen-dependent enzyme in the cholesterol-committed branch of the pathway. It is possible that constitutive activation of SM under oxygen-deficient conditions could reduce the toxicity of squalene and other sterol intermediates.

Reviewed by eLife

The role of Limch1 alternative splicing in skeletal muscle function

1. Matthew S. Penna
2. George G. Rodney
3. Rong-Chi Hu
4. Thomas A. Cooper

Reviewed by Review Commons

Translational buffering by ribosome stalling in upstream open reading frames

1. Ty A. Bottorff
2. Heungwon Park
3. Adam P. Geballe
4. Arvind Rasi Subramaniam

Reviewed by Review Commons

Drosophila cap-binding protein eiF4EHP promotes translation via a 3’UTR-dependent mechanism under hypoxia and contributes to fruit fly adaptation to oxygen variations

1. Manfei Liang
2. Clara Hody
3. Vanessa Yammine
4. Romuald Soin
5. Yuqiu Sun
6. Xing Lin
7. Xiaoying Tian
8. Romane Meurs
9. Camille Perdrau
10. Nadège Delacourt
11. Fabienne Andris
12. Louise Conrard
13. Véronique Kruys
14. Cyril Gueydan

Reviewed by Review Commons

Nucleotide-level linkage of transcriptional elongation and polyadenylation

1. Joseph V Geisberg
2. Zarmik Moqtaderi
3. Nova Fong
4. Benjamin Erickson
5. David L Bentley
6. Kevin Struhl

• Curated by eLife

  eLife assessment

  Giesberg and colleagues provide evidence both in yeast and human cells that fast elongation speeds of RNA polymerases result in a "downstream-shifted" poly(A) profile while the opposite is true for slower speeds of elongating polymerases. GC content of sequences downstream of poly(A) clusters influences the cluster profiles by affecting elongation and thus allowing more time for the 3'-cleavage complex to find the poly(A) site and form the transcript terminus. Although the findings presented in this manuscript are not surprising, they are new and contribute a missing piece to our knowledge of how the transcription machinery determines which poly(A) site to utilize at the end of genes.

Reviewed by eLife