Molecular Biology

Stimulation of the catalytic activity of the tyrosine kinase Btk by the adaptor protein Grb2

1. Laura M Nocka
2. Timothy J Eisen
3. Anthony T Iavarone
4. Jay T Groves
5. John Kuriyan

• Curated by eLife

  eLife assessment

  The authors' finding that Btk kinase activation relies on specific interactions with the Grb2 scaffold protein, including for recruitment into signaling clusters on membranes, is an unexpected finding that will potentially be of broad interest. The authors make a case for reinterpretation of the "Saraste dimer" of Btk as a signaling entity and assign roles to the component domains in the Src module in Btk activation, but the data provided are not yet fully convincing for this scenario.

Reviewed by eLife

PRMT5 links lipid metabolism to contractile function of skeletal muscles

1. Kun Ho Kim
2. Zhihao Jia
3. Madigan Snyder
4. Jingjuan Chen
5. Jiamin Qiu
6. Stephanie N Oprescu
7. Xiyue Chen
8. Sabriya A Syed
9. Feng Yue
10. Bruno T Roseguini
11. Anthony N Imbalzano
12. Changdeng Hu
13. Shihuan Kuang

Reviewed by Review Commons

Isoforms of the TAL1 transcription factor have different roles in hematopoiesis and cell growth

1. Aveksha Sharma
2. Shani Mistriel-Zerbib
3. Rauf Ahmad Najar
4. Eden Engal
5. Mercedes Bentata
6. Nadeen Taqatqa
7. Sara Dahan
8. Klil Cohen
9. Shiri Jaffe-Herman
10. Ophir Geminder
11. Mai Baker
12. Yuval Nevo
13. Inbar Plaschkes
14. Gillian Kay
15. Yotam Drier
16. Michael Berger
17. Maayan Salton

Reviewed by Review Commons

Ablation of palladin in adult heart causes dilated cardiomyopathy associated with intercalated disc abnormalities

1. Giuseppina Mastrototaro
2. Pierluigi Carullo
3. Jianlin Zhang
4. Beatrice Scellini
5. Nicoletta Piroddi
6. Simona Nemska
7. Maria Carmela Filomena
8. Simone Serio
9. Carol A Otey
10. Chiara Tesi
11. Fabian Emrich
12. Wolfgang A Linke
13. Corrado Poggesi
14. Simona Boncompagni
15. Marie-Louise Bang

• Curated by eLife

  eLife assessment

  This manuscript will be of interest to scientists who study cardiomyocyte homeostasis and contraction. It assesses the functional consequences of cardiomyocyte-specific knockout of Palladin, leading to the identification of a compensation mechanism when Palladin is deleted in embryogenesis, but not in adulthood. In addition, the authors identified new Palladin interactors, revealing a role for Palladin in the maintenance of intercalated disc structure.

Reviewed by eLife

Gene editing and scalable functional genomic screening in Leishmania species using the CRISPR/Cas9 cytosine base editor toolbox LeishBASEedit

1. Markus Engstler
2. Tom Beneke

• Curated by eLife

  eLife assessment

  Only few species of Leishmania, an important human pathogen, have an RNAi machinery, alternative methods are needed for genetic screens. The authors resent and validate a valuable method, based on the introduction of premature stop codons, that can be used for several different species. The results are very convincing, the data are solid, and the approach will be of interest to researchers studying any eukaryote that lacks the RNAi machinery.

Reviewed by eLife

RNA Polymerase II transcription independent of TBP in murine embryonic stem cells

1. James ZJ Kwan
2. Thomas F Nguyen
3. Anuli C Uzozie
4. Marek A Budzynski
5. Jieying Cui
6. Joseph MC Lee
7. Filip Van Petegem
8. Philipp F Lange
9. Sheila S Teves

• Curated by eLife

  eLife assessment

  This important study employs auxin-induced degradation to show that the TATA-binding protein (TBP) is not required for ongoing RNA polymerase II transcription nor heat-shock or retinoic acid-induced transcription, but that TBP is essential for RNA polymerase III transcription, with TBP-independent TFIID complexes being assembled and present at the transcription start sites of polymerase II-transcribed promoters. The evidence for the major claims is currently incomplete.

Reviewed by eLife

Subcytoplasmic location of translation controls protein output

1. Ellen L. Horste
2. Mervin M. Fansler
3. Ting Cai
4. Xiuzhen Chen
5. Sibylle Mitschka
6. Gang Zhen
7. Flora C.Y. Lee
8. Jernej Ule
9. Christine Mayr

Reviewed by Life Science Editors Foundation

Rapid and sensitive detection of native glycoRNAs

1. Helena Hemberger
2. Peiyuan Chai
3. Charlotta G. Lebedenko
4. Reese M. Caldwell
5. Benson M. George
6. Ryan A. Flynn

Reviewed by Arcadia Science

Integrating analog and digital modes of gene expression at Arabidopsis FLC

1. Rea L Antoniou-Kourounioti
2. Anis Meschichi
3. Svenja Reeck
4. Scott Berry
5. Govind Menon
6. Yusheng Zhao
7. John Fozard
8. Terri Holmes
9. Lihua Zhao
10. Huamei Wang
11. Matthew Hartley
12. Caroline Dean
13. Stefanie Rosa
14. Martin Howard

• Curated by eLife

  eLife assessment

  Regulation of gene expression in many biological systems occurs either through a binary mode where gene expression is either on or off (digital regulation), or through an analog mode leading to a graded modulation of gene expression. In this manuscript, the authors report how these two regulatory modes are integrated into a one-way switch pattern to control the expression of the Arabidopsis floral repressor gene FLOWERING LOCUS C (FLC). They suggest that an analog regulation in the autonomous pathway precedes a digital regulation conferred by Polycomb silencing before cold exposure, and this temporal switch correlates with the strength of transcription at the FLC locus in different genetic backgrounds.

Reviewed by eLife

Cohesin is involved in transcriptional repression of stage-specific genes in the human malaria parasite

1. Catarina Rosa
2. Parul Singh
3. Ameya Sinha
4. Peter R Preiser
5. Peter C Dedon
6. Sebastian Baumgarten
7. Artur Scherf
8. Jessica M Bryant

Reviewed by Review Commons