Molecular Biology

High-precision mapping of nuclear pore-chromatin interactions reveals new principles of genome organization at the nuclear envelope

1. Swati Tyagi
2. Juliana S. Capitanio
3. Jiawei Xu
4. Fei Chen
5. Rahul Sharma
6. Jialiang Huang
7. Martin W. Hetzer

• Curated by eLife

  eLife assessment

  This manuscript describes important findings that will impact our understanding of nuclear pore complex (NPC)-genome interactions and how nuclear pore proteins (nucleoporins) impact super enhancer function. The authors develop a clever new approach termed NPC-DamID to map chromatin-NPC interactions that has several important advantages over existing techniques and may be used in contexts that are incompatible with genetic manipulation. The authors provide compelling evidence that nucleoporins interact with super enhancers in multiple cell types and also suggest that the phase separation behavior of nucleoporins contributes to the hierarchical organization of super enhancers, which is exciting and will stimulate additional work.

Reviewed by eLife

TCR-pMHC complex formation triggers CD3 dynamics

1. Floris J. van Eerden
2. Aalaa Alrahman Sherif
3. Mara Anais Llamas-Covarrubias
4. Arthur Millius
5. Xiuyuan Lu
6. Shigenari Ishizuka
7. Sho Yamasaki
8. Daron M. Standley

• Curated by eLife

  eLife assessment

  There was a range of opinion among three highly expert reviewers from different perspectives in the field. This is a significant topic and it was felt that the contribution at present is valuable to those in the field. However, it was agreed after consultation that the description of the simulation methodology was inadequate.

Reviewed by eLife

p38γ and p38δ modulate innate immune response by regulating MEF2D activation

1. Alejandra Escós
2. Ester Diaz-Mora
3. Michael Pattison
4. Pilar Fajardo
5. Diego González-Romero
6. Ana Risco
7. José Martín-Gómez
8. Éric Bonneil
9. Nahum Sonenberg
10. Seyed Mehdi Jafarnejad
11. Juan José Sanz-Ezquerro
12. Steven C Ley
13. Ana Cuenda

• Curated by eLife

  eLife assessment

  The authors have established a model for studying p38g/d signaling, which is an important accomplishment given that previous models have been compromised by changes in the TPL2/ERK pathway. Compelling evidence is presented to support the conclusions.

Reviewed by eLife

The obesity-linked human lncRNA AATBC regulates adipocyte plasticity by stimulating mitochondrial dynamics and respiration

1. Maude Giroud
2. Stefan Kotschi
3. Yun Kwon
4. Ophélia Le Thuc
5. Anne Hoffmann
6. Manuel Gil-Lozano
7. Michael Karbiener
8. Juan Carlos Higareda-Almaraz
9. Sajjad Khani
10. Daniel Tews
11. Pamela Fischer-Posovszky
12. Wenfei Sun
13. Hua Dong
14. Adhideb Ghosh
15. Christian Wolfrum
16. Martin Wabitsch
17. Kirsi A. Virtanen
18. Matthias Blüher
19. Søren Nielsen
20. Anja Zeigerer
21. Cristina García-Cáceres
22. Marcel Scheideler
23. Stephan Herzig
24. Alexander Bartelt

Reviewed by Review Commons

The yeast RNA methylation complex consists of conserved yet reconfigured components with m6A-dependent and independent roles

1. Imke Ensinck
2. Alexander Maman
3. Waleed S Albihlal
4. Michelangelo Lassandro
5. Giulia Salzano
6. Theodora Sideri
7. Steven A Howell
8. Enrica Calvani
9. Harshil Patel
10. Guy Bushkin
11. Markus Ralser
12. Ambrosius P Snijders
13. Mark Skehel
14. Ana Casañal
15. Schraga Schwartz
16. Folkert J van Werven

• Curated by eLife

  eLife assessment

  This fundamental study identifies the components of the N6-methyladenosine methyltransferase complexes in yeasts, with major differences with the same complexes in mammals and flies. The evidence supporting the conclusions is convincing, with rigorous high-throughput sequencing approaches and detailed functional analysis. This work will be of broad interest to colleagues in the RNA modification and meiosis fields.

Reviewed by eLife

Annexin A6 mediates calcium-dependent exosome secretion during plasma membrane repair

1. Justin Krish Williams
2. Jordan Matthew Ngo
3. Isabelle Madeline Lehman
4. Randy Schekman

• Curated by eLife

  eLife assessment

  This compelling study brings together two earlier observations: that Ca2+ influx can trigger exosome release from multivesicular bodies, and that plasma membrane repair after wounding requires Ca2+ and involves Ca2+-binding annexin proteins. This important work takes these earlier findings in an interesting new direction by showing that exosome release from MVBs is also triggered by Ca2+ influx during plasma membrane wounding and requires the annexin isoform ANX6. The study suggests a few possible mechanisms (such as Ca2+-dependent tethering of MVBs to the plasma membrane by ANX6) and raises the interesting possibility that cell injury and repair may contribute to the release of exosomes into biological fluids.

Reviewed by eLife

The nucleolar protein GNL3 prevents resection of stalled replication forks

1. Rana Lebdy
2. Marine Canut
3. Julie Patouillard
4. Jean‐Charles Cadoret
5. Anne Letessier
6. Josiane Ammar
7. Jihane Basbous
8. Serge Urbach
9. Benoit Miotto
10. Angelos Constantinou
11. Raghida Abou Merhi
12. Cyril Ribeyre

Reviewed by Review Commons

Using machine learning to predict protein–protein interactions between a zombie ant fungus and its carpenter ant host

1. Ian Will
2. William C. Beckerson
3. Charissa de Bekker

Reviewed by Arcadia Science

From silence to song: Testosterone triggers extensive transcriptional changes in the female canary HVC

1. Meng‐Ching Ko
2. Carolina Frankl‐Vilches
3. Antje Bakker
4. Nina Sohnius‐Wilhelmi
5. Pepe Alcami
6. Manfred Gahr

• Curated by eLife

  eLife assessment

  This paper is of interest to neuroscientists studying the effects of sex hormones on neural and behavioral plasticity, and more specifically to researchers studying the neural mechanisms underlying birdsong plasticity. The experimental design is excellent, and the work provides a comprehensive resource for understanding the intersection between testosterone's influence on gene expression and behavior. However, the work also makes claims concerning cellular effects and gene regulatory mechanisms that extend beyond the data and under the current analyses are not rigorously supported.

Reviewed by eLife

Control of telomere length in yeast by SUMOylated PCNA and the Elg1 PCNA unloader

1. Pragyan Singh
2. Inbal Gazy
3. Martin Kupiec

• Curated by eLife

  eLife assessment

  This important study aims to discover the mechanisms governing the switch between conventional DNA replication and the specialized mechanism of telomere end replication. Solid genetic and biochemical assays suggest an interplay between sumoylated PCNA and chromosome terminal capping proteins. The questions addressed have implications for several fields, such as genome stability.

Reviewed by eLife