Microbiology

STING mediates immune responses in the closest living relatives of animals

1. Arielle Woznica
2. Ashwani Kumar
3. Carolyn R Sturge
4. Chao Xing
5. Nicole King
6. Julie K Pfeiffer

• Curated by eLife

  Evaluation Summary:

  The authors addressed the role of a STING ortholog in antimicrobial defense of choanoflagellates. The analysis of the response of the choanoflagellate Monosiga brevicollis to a variety of bacterial species revealed that exposure of M. brevicollis to Pseudomonas aeruginosa conditioned medium results in choanoflagellate death and the authors found that this is dependent on the newly discovered ortholog of STING. Characterization reveals that the STING response can be induced by 2'3' cGAMP, which parallels the activation of STING in diverse species. In addition, the finding that cyclic dinucleotide treatment induces autophagy also has parallels with the effector pathways observed in other organisms. There are a number of strengths as outlined by the Reviewers. First, the development of a Choanaoflagellate model system to study innate immunity, second with the development of genetics for M. brevicollis, third, the demonstration of a functional STING system in one of the closest relatives to animals and fourth, that cell death occurs in response to cyclic dinucleotides.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife, preLights

Immunometabolic hijacking of immune cells by a Pseudomonas aeruginosa quorum-sensing signal

1. Arunava Bandyopadhaya
2. Vijay K Singh
3. Arijit Chakraborty
4. A. Aria Tzika
5. Laurence G Rahme

• Curated by eLife

  Evaluation Summary:

  In a previous study the authors showed that the quorum sensing signal molecule 2-aminoacetophenone (2-AA) produced by Pseudomonas aeruginosa enables persistence in host tissue of this pathogen. They propose that this effect depends on a Warburg-like metabolic reprogramming effect in macrophages.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife, preLights

Complete protection by a single-dose skin patch–delivered SARS-CoV-2 spike vaccine

1. Christopher L. D. McMillan
2. Jovin J. Y. Choo
3. Adi Idris
4. Aroon Supramaniam
5. Naphak Modhiran
6. Alberto A. Amarilla
7. Ariel Isaacs
8. Stacey T. M. Cheung
9. Benjamin Liang
10. Helle Bielefeldt-Ohmann
11. Armira Azuar
12. Dhruba Acharya
13. Gabrielle Kelly
14. Germain J. P. Fernando
15. Michael J. Landsberg
16. Alexander A. Khromykh
17. Daniel Watterson
18. Paul R. Young
19. Nigel A. J. McMillan
20. David A. Muller

Reviewed by ScreenIT

The SARS-CoV-2 variants associated with infections in India, B.1.617, show enhanced spike cleavage by furin

1. Thomas P. Peacock
2. Carol M. Sheppard
3. Jonathan C. Brown
4. Niluka Goonawardane
5. Jie Zhou
6. Max Whiteley
7. PHE Virology Consortium
8. Thushan I. de Silva
9. Wendy S. Barclay

Reviewed by ScreenIT

Spike mutation T403R allows bat coronavirus RaTG13 to use human ACE2

1. Fabian Zech
2. Daniel Schniertshauer
3. Christoph Jung
4. Alexandra Herrmann
5. Qinya Xie
6. Rayhane Nchioua
7. Caterina Prelli Bozzo
8. Meta Volcic
9. Lennart Koepke
10. Jana Krüger
11. Sandra Heller
12. Alexander Kleger
13. Timo Jacob
14. Karl-Klaus Conzelmann
15. Armin Ensser
16. Konstantin M.J. Sparrer
17. Frank Kirchhoff

Reviewed by ScreenIT

SARS-CoV-2 spreads through cell-to-cell transmission

1. Cong Zeng
2. John P. Evans
3. Tiffany King
4. Yi-Min Zheng
5. Eugene M. Oltz
6. Sean P. J. Whelan
7. Linda J. Saif
8. Mark E. Peeples
9. Shan-Lu Liu

Reviewed by ScreenIT

Kite-Shaped Molecules Block SARS-CoV-2 Cell Entry at a Post-Attachment Step

1. Shiu-Wan Chan
2. Talha Shafi
3. Robert C. Ford

Reviewed by ScreenIT

Species-Specific Molecular Barriers to SARS-CoV-2 Replication in Bat Cells

1. Sophie-Marie Aicher
2. Felix Streicher
3. Maxime Chazal
4. Delphine Planas
5. Dongsheng Luo
6. Julian Buchrieser
7. Monika Nemcova
8. Veronika Seidlova
9. Jan Zukal
10. Jordi Serra-Cobo
11. Dominique Pontier
12. Bertrand Pain
13. Gert Zimmer
14. Olivier Schwartz
15. Philippe Roingeard
16. Jiri Pikula
17. Laurent Dacheux
18. Nolwenn Jouvenet

Reviewed by ScreenIT

A suitable murine model for studying respiratory coronavirus infection and therapeutic countermeasures in BSL-2 laboratories

1. Ana Cláudia dos Santos Pereira Andrade
2. Gabriel Henrique Campolina-Silva
3. Celso Martins Queiroz-Junior
4. Leonardo Camilo de Oliveira
5. Larisse de Souza Barbosa Lacerda
6. Jordane Clarisse Pimenta
7. Filipe Resende Oliveira de Souza
8. Ian de Meira Chaves
9. Ingredy Beatriz Passos
10. Danielle Cunha Teixeira
11. Paloma Graziele Bittencourt-Silva
12. Priscila Aparecida Costa Valadão
13. Leonardo Rossi-Oliveira
14. Maisa Mota Antunes
15. André Felipe Almeida Figueiredo
16. Natália Teixeira Wnuk
17. Jairo R. Temerozo
18. André Costa Ferreira
19. Allysson Cramer
20. Cleida Aparecida Oliveira
21. Ricardo Durães-Carvalho
22. Clarice Weis Arns
23. Pedro Pires Goulart Guimarães
24. Guilherme Mattos Jardim Costa
25. Gustavo Batista de Menezes
26. Cristina Guatimosim
27. Glauber Santos Ferreira da Silva
28. Thiago Moreno L. Souza
29. Breno Rocha Barrioni
30. Marivalda de Magalhães Pereira
31. Lirlândia Pires de Sousa
32. Mauro Martins Teixeira
33. Vivian Vasconcelos Costa

Reviewed by ScreenIT

Bronchial epithelia from adults and children: SARS-CoV-2 spread via syncytia formation and type III interferon infectivity restriction

1. Guillaume Beucher
2. Marie-Lise Blondot
3. Alexis Celle
4. Noémie Pied
5. Patricia Recordon-Pinson
6. Pauline Esteves
7. Muriel Faure
8. Mathieu Métifiot
9. Sabrina Lacomme
10. Denis Dacheux
11. Derrick R. Robinson
12. Gernot Längst
13. Fabien Beaufils
14. Marie-Edith Lafon
15. Patrick Berger
16. Marc Landry
17. Denis Malvy
18. Thomas Trian
19. Marie-Line Andreola
20. Harald Wodrich

Reviewed by ScreenIT