Microbiology

aCPSF1 cooperates with terminator U-tract to dictate archaeal transcription termination efficacy

1. Jie Li
2. Lei Yue
3. Zhihua Li
4. Wenting Zhang
5. Bing Zhang
6. Fangqing Zhao
7. Xiuzhu Dong

• Curated by eLife

  Evaluation Summary:

  This study presents new evidence that support a model of aCPSF1-dependent transcription termination in Archaea. Archaeal transcription termination is shown to rely on both poly-U tract terminator signals and the endoribonuclease aCPSF1 of the β-CASP family. This mechanism resembles the eukaryal RNAP II termination process. These new insights fill a gap in our understanding of the mechanism of transcription termination in Archaea and they are of general importance for the RNA biology community.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their names with the authors.)

Reviewed by eLife

A bacterial derived plant- mimicking cytokinin hormone regulates social behaviour in a rice pathogen

1. Sohini Deb
2. Chandan Kumar
3. Rahul Kumar
4. Amandeep Kaur
5. Palash Ghosh
6. Gopaljee Jha
7. Prabhu B. Patil
8. Subhadeep Chatterjee
9. Hitendra K. Patel
10. Ramesh V. Sonti

• Curated by eLife

  Evaluation Summary:

  The major finding of this manuscript is that cytokinin produced by a bacterial plant pathogen affects bacterial growth and physiology. Production of cytokinin is linked to the well-known type three effector XopQ, which has primarily been studied for its function inside plant cells. The authors provide evidence that XopQ is required for the pathogen Xanthomonas oryzae to produce cytokinin in culture, and that cytokinin production controls whether or not the bacterium engages in planktonic growth or biofilm formation (i.e., biofilms form in the absence of cytokinin). These data indicate that bacterially produced cytokinins affect bacterial physiology, indicating that these hormones control signaling beyond photosynthetic organisms. The findings are of interest both to those studying plant-pathogen interactions and to microbiologists in general.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Post-entry, spike-dependent replication advantage of B.1.1.7 and B.1.617.2 over B.1 SARS-CoV-2 in an ACE2-deficient human lung cell line

1. Daniela Niemeyer
2. Simon Schroeder
3. Kirstin Friedmann
4. Friderike Weege
5. Jakob Trimpert
6. Anja Richter
7. Saskia Stenzel
8. Jenny Jansen
9. Jackson Emanuel
10. Julia Kazmierski
11. Fabian Pott
12. Lara M. Jeworowski
13. Ruth Olmer
14. Mark-Christian Jaboreck
15. Beate Tenner
16. Jan Papies
17. Julian Heinze
18. Felix Walper
19. Marie L. Schmidt
20. Nicolas Heinemann
21. Elisabeth Möncke-Buchner
22. Talitha Veith
23. Morris Baumgardt
24. Karen Hoffmann
25. Marek Widera
26. Tran Thi Nhu Thao
27. Anita Balázs
28. Jessica Schulze
29. Christin Mache
30. Markus Morkel
31. Sandra Ciesek
32. Leif G. Hanitsch
33. Marcus A. Mall
34. Andreas C. Hocke
35. Volker Thiel
36. Klaus Osterrieder
37. Thorsten Wolff
38. Ulrich Martin
39. Victor M. Corman
40. Marcel A. Müller
41. Christine Goffinet
42. Christian Drosten

Reviewed by ScreenIT

S100A8 and S100A9, biomarkers of SARS-Cov2-infected patients, suppress HIV replication in primary macrophages

1. Raphael M. Oguariri
2. Terrence W. Brann
3. Joseph W. Adelsberger
4. Qian Chen
5. Suranjana Goswami
6. Anthony R. Mele
7. Tomozumi Imamichi

Reviewed by ScreenIT

Nucleocapsid mutations in SARS-CoV-2 augment replication and pathogenesis

1. Bryan A. Johnson
2. Yiyang Zhou
3. Kumari G. Lokugamage
4. Michelle N. Vu
5. Nathen Bopp
6. Patricia A. Crocquet-Valdes
7. Birte Kalveram
8. Craig Schindewolf
9. Yang Liu
10. Dionna Scharton
11. Jessica A. Plante
12. Xuping Xie
13. Patricia Aguilar
14. Scott C. Weaver
15. Pei-Yong Shi
16. David H. Walker
17. Andrew L. Routh
18. Kenneth S. Plante
19. Vineet D. Menachery

Reviewed by ScreenIT

Bees can be trained to identify SARS-CoV-2 infected samples

1. Evangelos Kontos
2. Aria Samimi
3. Renate W. Hakze-van der Honing
4. Jan Priem
5. Aurore Avarguès-Weber
6. Alexander Haverkamp
7. Marcel Dicke
8. Jose L. Gonzales
9. Wim H. M. van der Poel

Reviewed by ScreenIT

A monoclonal antibody that neutralizes SARS-CoV-2 variants, SARS-CoV, and other sarbecoviruses

1. Pengfei Wang
2. Ryan G. Casner
3. Manoj S. Nair
4. Jian Yu
5. Yicheng Guo
6. Maple Wang
7. Jasper F.-W. Chan
8. Gabriele Cerutti
9. Sho Iketani
10. Lihong Liu
11. Zizhang Sheng
12. Zhiwei Chen
13. Kwok-Yung Yuen
14. Peter D. Kwong
15. Yaoxing Huang
16. Lawrence Shapiro
17. David D. Ho

Reviewed by ScreenIT

The adenosine analogue prodrug ATV006 is orally bioavailable and has potent preclinical efficacy against SARS-CoV-2 and its variants

1. Liu Cao
2. Yingjun Li
3. Sidi Yang
4. Guanguan Li
5. Qifan Zhou
6. Jing Sun
7. Tiefeng Xu
8. Yujian Yang
9. Tiaozhen Zhu
10. Siyao Huang
11. Yanxi Ji
12. Feng Cong
13. Yinzhu Luo
14. Yujun Zhu
15. Hemi Luan
16. Huan Zhang
17. Jingdiao Chen
18. Xue Liu
19. Ping Wang
20. Yang Yu
21. Fan Xing
22. Bixia Ke
23. Huanying Zheng
24. Xiaoling Deng
25. Wenyong Zhang
26. Chun-Mei Li
27. Yu Zhang
28. Jincun Zhao
29. Xumu Zhang
30. Deyin Guo

Reviewed by ScreenIT

A SARS-CoV-2 variant elicits an antibody response with a shifted immunodominance hierarchy

1. Allison J. Greaney
2. Tyler N. Starr
3. Rachel T. Eguia
4. Andrea N. Loes
5. Khadija Khan
6. Farina Karim
7. Sandile Cele
8. John E. Bowen
9. Jennifer K. Logue
10. Davide Corti
11. David Veesler
12. Helen Y. Chu
13. Alex Sigal
14. Jesse D. Bloom

Reviewed by ScreenIT

Clp protease and antisense RNA jointly regulate the global regulator CarD to mediate mycobacterial starvation response

1. Xinfeng Li
2. Fang Chen
3. Xiaoyu Liu
4. Jinfeng Xiao
5. Binda T Andongma
6. Qing Tang
7. Xiaojian Cao
8. Shan-Ho Chou
9. Michael Y Galperin
10. Jin He

• Curated by eLife

  Evaluation Summary:

  CarD is an RNA polymerase interacting protein that is essential for mycobacterial viability, the levels of which are important for controlling gene expression in mycobacteria during various stress conditions. This study reports two mechanisms that regulate levels of CarD under stress conditions, including starvation. The authors report that CarD levels are tightly regulated and that there was a dramatic decrease in the levels of CarD when cells switched from the nutrient-rich to the starvation condition. They discovered two synergistic mechanisms that led to this dramatic decrease in CarD. The first is SigF-dependent induction of antisense RNA of CarD (AscarD), which inhibits CarD translation and a second mechanism involving Clp protease-mediated degradation of intracellular CarD. The work will be of interest to researchers studying non-coding RNAs, microbial gene expression, physiology and stress response.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife