Immunology

mRNA Vaccination Induces Durable Immune Memory to SARS-CoV-2 with Continued Evolution to Variants of Concern

1. Rishi R. Goel
2. Mark M. Painter
3. Sokratis A. Apostolidis
4. Divij Mathew
5. Wenzhao Meng
6. Aaron M. Rosenfeld
7. Kendall A. Lundgreen
8. Arnold Reynaldi
9. David S. Khoury
10. Ajinkya Pattekar
11. Sigrid Gouma
12. Leticia Kuri-Cervantes
13. Philip Hicks
14. Sarah Dysinger
15. Amanda Hicks
16. Harsh Sharma
17. Sarah Herring
18. Scott Korte
19. Amy E. Baxter
20. Derek A. Oldridge
21. Josephine R. Giles
22. Madison E. Weirick
23. Christopher M. McAllister
24. Moses Awofolaju
25. Nicole Tanenbaum
26. Elizabeth M. Drapeau
27. Jeanette Dougherty
28. Sherea Long
29. Kurt D’Andrea
30. Jacob T. Hamilton
31. Maura McLaughlin
32. Justine C. Williams
33. Sharon Adamski
34. Oliva Kuthuru
35. The UPenn COVID Processing Unit
36. Ian Frank
37. Michael R. Betts
38. Laura A. Vella
39. Alba Grifoni
40. Daniela Weiskopf
41. Alessandro Sette
42. Scott E. Hensley
43. Miles P. Davenport
44. Paul Bates
45. Eline T. Luning Prak
46. Allison R. Greenplate
47. E. John Wherry

Reviewed by ScreenIT

De novo synthesized polyunsaturated fatty acids operate as both host immunomodulators and nutrients for Mycobacterium tuberculosis

1. Thomas Laval
2. Laura Pedró-Cos
3. Wladimir Malaga
4. Laure Guenin-Macé
5. Alexandre Pawlik
6. Véronique Mayau
7. Hanane Yahia-Cherbal
8. Océane Delos
9. Wafa Frigui
10. Justine Bertrand-Michel
11. Christophe Guilhot
12. Caroline Demangel

• Curated by eLife

  Evaluation Summary:

  In this study, the authors highlight a role for de novo biosynthesis of Poly-unsaturated Fatty Acids and the consequence effect of these metabolites on the production of arachidonic acid. The increased bio-availability of arachidonic acid seemingly promotes mycobacterial growth whilst inhibition of arachidonic acid formation, and its resultant downstream eicosanoid products, affect macrophage function but somewhat surprisingly do not affect growth of M. tuberculosis in macrophages or in mice. The uptake of the different classes of fatty acids in axenic culture as well as in macrophages is explored and the authors demonstrate that the Mce1 transporter is largely responsible for their uptake during in vitro growth but only plays a partial role in their uptake during growth of the pathogen in host cells. This work will be of interest to bacteriologists and those studying infectious diseases.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

Protein kinase Cδ is essential for the IgG response against T-cell-independent type 2 antigens and commensal bacteria

1. Saori Fukao
2. Kei Haniuda
3. Hiromasa Tamaki
4. Daisuke Kitamura

• Curated by eLife

  Evaluation Summary:

  The unexpected key finding is that T-cell-independent B cell activation engages the intracellular protein kinase PKC-delta to induce the necessary machinery of altered gene transcription culminating in IgG production. The methodology and the experimental setting are well-controlled, although how exactly B-cell stimulation by T-cell-independent antigens triggers PKC-delta remains to be elucidated.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

An endogenously activated antiviral state restricts SARS-CoV-2 infection in differentiated primary airway epithelial cells

1. Lindsay Broadbent
2. Connor G. G. Bamford
3. Guillermo Lopez Campos
4. Sheerien Manzoor
5. David Courtney
6. Ahlam Ali
7. Olivier Touzelet
8. Conall McCaughey
9. Ken Mills
10. Ultan F. Power

Reviewed by ScreenIT

Platelet proteome analysis reveals an early hyperactive phenotype in SARS-CoV-2-infected humanized ACE2 mice

1. Saravanan Subramaniam
2. Ryan Matthew Hekman
3. Archana Jayaraman
4. Aoife Kateri O’Connell
5. Paige Montanaro
6. Benjamin Blum
7. Devin Kenney
8. Maria Ericsson
9. Katya Ravid
10. Nicholas A Crossland
11. Andrew Emili
12. Florian Douam
13. Markus Bosmann

Reviewed by ScreenIT

Pandemic, Epidemic, Endemic: B Cell Repertoire Analysis Reveals Unique Anti-Viral Responses to SARS-CoV-2, Ebola and Respiratory Syncytial Virus

1. Alexander Stewart
2. Emma Sinclair
3. Joseph Chi-Fung Ng
4. Joselli Silva O’Hare
5. Audrey Page
6. Ilaria Serangeli
7. Christian Margreitter
8. Federica Orsenigo
9. Katherine Longman
10. Cecile Frampas
11. Catia Costa
12. Holly-May Lewis
13. Nora Kasar
14. Bryan Wu
15. David Kipling
16. Peter JM Openshaw
17. Christopher Chiu
18. J Kenneth Baillie
19. Janet T. Scott
20. Malcolm G. Semple
21. Melanie J. Bailey
22. Franca Fraternali
23. Deborah K. Dunn-Walters

Reviewed by ScreenIT

T Cell Predominant Response to AAV-Spike Protects hACE2 Mice from SARS-CoV-2 Pneumonia

1. Christopher D. Greer
2. Coral M. Kasden
3. Leon Morales
4. Kendall A. Lundgreen
5. Philip D. Hicks
6. Lilia J. Carpenter
7. Cristhian Salas-Quinchucua
8. Elisia D. Tichy
9. Albert M. Maguire
10. Jennifer G. Hoffman
11. Robin J. Bailey
12. Shangzhen Zhou
13. Angela Luo
14. Steven J. Chomistek
15. Benjamin W. Kozyak
16. Charles R. Bridges
17. Gilad S. Gordon
18. Geoffrey C. Tabin
19. Paul F. Bates
20. Jorge E. Osorio
21. Jean Bennett
22. Hansell H. Stedman

Reviewed by ScreenIT

Molecular Basis of a Dominant SARS-CoV-2 Spike-Derived Epitope Presented by HLA-A*02:01 Recognised by a Public TCR

1. Christopher Szeto
2. Andrea T. Nguyen
3. Christian A. Lobos
4. Demetra S. M. Chatzileontiadou
5. Dhilshan Jayasinghe
6. Emma J. Grant
7. Alan Riboldi-Tunnicliffe
8. Corey Smith
9. Stephanie Gras

Reviewed by ScreenIT

Durability of SARS-CoV-2-specific T cell responses at 12-months post-infection

1. Zhongyan Lu
2. Eric D. Laing
3. Jarina Pena-Damata
4. Katherine Pohida
5. Marana S. Tso
6. Emily C. Samuels
7. Nusrat J. Epsi
8. Batsukh Dorjbal
9. Camille Lake
10. Stephanie A. Richard
11. Ryan C. Maves
12. David A. Lindholm
13. Julia Rozman
14. Caroline English
15. Nikhil Huprikar
16. Katrin Mende
17. Rhonda E. Colombo
18. Christopher J. Colombo
19. Christopher C. Broder
20. Anuradha Ganesan
21. Charlotte A. Lanteri
22. Brian K. Agan
23. David Tribble
24. Mark P. Simons
25. Clifton L. Dalgard
26. Paul W. Blair
27. Josh Chenoweth
28. Simon D. Pollett
29. Andrew L. Snow
30. Timothy H. Burgess
31. Allison M.W. Malloy
32. the EPICC COVID-19 Cohort Study Group

Reviewed by ScreenIT

ACE2 is the critical in vivo receptor for SARS-CoV-2 in a novel COVID-19 mouse model with TNF- and IFNγ-driven immunopathology

1. Riem Gawish
2. Philipp Starkl
3. Lisabeth Pimenov
4. Anastasiya Hladik
5. Karin Lakovits
6. Felicitas Oberndorfer
7. Shane JF Cronin
8. Anna Ohradanova-Repic
9. Gerald Wirnsberger
10. Benedikt Agerer
11. Lukas Endler
12. Tümay Capraz
13. Jan W Perthold
14. Domagoj Cikes
15. Rubina Koglgruber
16. Astrid Hagelkruys
17. Nuria Montserrat
18. Ali Mirazimi
19. Louis Boon
20. Hannes Stockinger
21. Andreas Bergthaler
22. Chris Oostenbrink
23. Josef M Penninger
24. Sylvia Knapp

• Curated by eLife

  Evaluation Summary:

  To establish a mouse model for the SARS-CoV-2 infection, Gawish and colleagues performed serial passage of a human virus isolate in mice. They show that the mouse-adapted SARS-CoV-2 variant remains dependent on ACE2 for efficient infection and recapitulates some clinical characteristics of COVID-19. In addition, they demonstrate that inhalation of recombinant ACE2 protected mice from mouse COVID-19 suggesting this this model will be useful for the testing of antiviral agents.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife, ScreenIT