1. Fitness effects of CRISPR endonucleases in Drosophila melanogaster populations

    This article has 11 authors:
    1. Anna M Langmüller
    2. Jackson Champer
    3. Sandra Lapinska
    4. Lin Xie
    5. Matthew Metzloff
    6. Samuel E Champer
    7. Jingxian Liu
    8. Yineng Xu
    9. Jie Du
    10. Andrew G Clark
    11. Philipp W Messer
    This article has been curated by 1 group:
    • Curated by eLife

      Evaluation Summary:

      The issue of general fitness effects in organisms expressing Cas9 enzymes as part of gene drive genetic control strategies is important, particularly in the emerging field of vector control. This manuscript reports experiments aimed at teasing apart such effects in a Drosophila model system, providing evidence that off-target effects predominate, which may be ameliorated by utilising high-fidelity nucleases, but a more detailed analysis of data and justification for some of the assumptions, especially some direct evidence of off-target cleavage, are still needed to support the authors' inferences. It is currently also not entirely clear how the lines were generated and tested. Finally, additional modelling to include scenarios where the initial frequency of the drive allele is very low (as would be the case for an actual release) would help to strengthen the conclusions.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

    Reviewed by eLife

    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
  2. A single nucleotide variant in the PPARγ-homolog Eip75B affects fecundity in Drosophila

    This article has 4 authors:
    1. Katja M Hoedjes
    2. Hristina Kostic
    3. Thomas Flatt
    4. Laurent Keller
    This article has been curated by 1 group:
    • Curated by eLife

      Evaluation Summary:

      Hoedjes et al. examine how a single nucleotide variant in a regulatory region upstream of the Eip75B gene influences key aspects of life history in Drosophila, using RNAi knockdowns, inbred lines and CRISPR/Cas9 allele replacement at the endogenous locus. This study represents one of the very few examples in animals where the effect of a naturally segregating single nucleotide variant on a complex trait is carefully quantified.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

    Reviewed by eLife

    This article has 3 evaluationsAppears in 1 listLatest version Latest activity
  3. Lung evolution in vertebrates and the water-to-land transition

    This article has 13 authors:
    1. Camila Cupello
    2. Tatsuya Hirasawa
    3. Norifumi Tatsumi
    4. Yoshitaka Yabumoto
    5. Pierre Gueriau
    6. Sumio Isogai
    7. Ryoko Matsumoto
    8. Toshiro Saruwatari
    9. Andrew King
    10. Masato Hoshino
    11. Kentaro Uesugi
    12. Masataka Okabe
    13. Paulo M Brito
    This article has been curated by 1 group:
    • Curated by eLife

      Evaluation Summary:

      This study used valuable materials from five osteichthyan vertebrate species and investigated their lung morphology in them. The comparison of the observations suggest an origin of the lung as an unpaired organ, with the present-day paired forms in amniotes being a result of secondary modification. The sound morphological comparison presented provides valuable insight into the evolution of the lung. The work will be of interest to colleagues studying vertebrate evolution.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 and Reviewer #3 agreed to share their name with the authors.)

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  4. Investigating the evolutionary origins of the first three SARS-CoV-2 variants of concern

    This article has 5 authors:
    1. Mahan Ghafari
    2. Qihan Liu
    3. Arushi Dhillon
    4. Aris Katzourakis
    5. Daniel B. Weissman

    Reviewed by ScreenIT

    This article has 1 evaluationAppears in 1 listLatest version Latest activity
  5. Admixture of evolutionary rates across a butterfly hybrid zone

    This article has 4 authors:
    1. Tianzhu Xiong
    2. Xueyan Li
    3. Masaya Yago
    4. James Mallet
    This article has been curated by 1 group:
    • Curated by eLife

      Evaluation Summary:

      The authors leverage theory, simulations, and empirical population genomics to evaluate what are the consequences of differences in substitution rates in hybridizing species. This is a largely overlooked phenomenon. This study highlights the issue and demonstrates that two hybridizing species of Papilio have differences in their substitution rates. The work will be of interest to a large group of evolutionary biologists, especially those studying evolution at the whole-genome level.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  6. Widespread introgression across a phylogeny of 155 Drosophila genomes

    This article has 15 authors:
    1. Anton Suvorov
    2. Bernard Y. Kim
    3. Jeremy Wang
    4. Ellie E. Armstrong
    5. David Peede
    6. Emmanuel R.R. D’Agostino
    7. Donald K. Price
    8. Peter J. Waddell
    9. Michael Lang
    10. Virginie Courtier-Orgogozo
    11. Jean R. David
    12. Dmitri Petrov
    13. Daniel R. Matute
    14. Daniel R. Schrider
    15. Aaron A. Comeault
    This article has been curated by 1 group:
    • Curated by eLife

      Evaluation Summary:

      The authors present an impressive view of introgression across the Drosophila clade. There is strong support for signals of introgression along numerous branches of the phylogeny. However, the placement of these introgression events on the phylogeny and their impact on genome-wide patterns of relatedness are less clear.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  7. The spatiotemporal patterns of major human admixture events during the European Holocene

    This article has 3 authors:
    1. Manjusha Chintalapati
    2. Nick Patterson
    3. Priya Moorjani
    This article has been curated by 1 group:
    • Curated by eLife

      Evaluation Summary:

      This manuscript presents DATES, a method to infer the timing of admixture events using genetic data from present-day or ancient individuals. This is a robust method that is useful in the field of paleogenomics and outperforms existing methods. In this manuscript, DATES is applied to >1000 ancient human genomes to characterize major admixture events during the European Holocene. This work will be of interest to scholars in the fields of population genetics, paleogenomics, archeology, biological anthropology, and history.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

    Reviewed by eLife

    This article has 3 evaluationsAppears in 2 listsLatest version Latest activity
  8. Convergent mosaic brain evolution is associated with the evolution of novel electrosensory systems in teleost fishes

    This article has 2 authors:
    1. Erika L Schumacher
    2. Bruce A Carlson
    This article has been curated by 1 group:
    • Curated by eLife

      Evaluation Summary:

      This ms examines changes in brain region size in several groups of weakly electric fishes, the Mormyroidea, and the Gymnotiformes and weakly electric catfishes (Synodontis spp.), which evolved electroreception independently of mormyrids. These are an interesting group for examination of mosaic growth. Many analyses are thoughtful and well executed, but there is some concern about whether the observed volumetric decreases are a consequence of the method.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

    Reviewed by eLife

    This article has 6 evaluationsAppears in 1 listLatest version Latest activity
  9. Squamation and scale morphology at the root of jawed vertebrates

    This article has 2 authors:
    1. Yajing Wang
    2. Min Zhu
    This article has been curated by 1 group:
    • Curated by eLife

      Evaluation Summary:

      This manuscript will be of strong interest to scientists studying the development of early jawed vertebrates, in particular the extent and structure of their dermal skeleton, but it will also interest a broader audience, given how it connects modern-day morphological techniques to paleobiology. The authors provide the most complete account to date of the body scales of an antiarch stem-group gnathostome; this is the first work to model in 3-D the entire scale cover of such a fossil fish. The authors show that the body scales are varied in form, regionalized and that they comprise two main tissue layers. Based on this they argue that these conditions are plesiomorphic for antiarchs and the gnathostome crown group.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their name with the authors.)

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  10. Male rat leukocyte population dynamics predict a window for intervention in aging

    This article has 10 authors:
    1. Hagai Yanai
    2. Christopher Dunn
    3. Bongsoo Park
    4. Christopher Coletta
    5. Ross A McDevitt
    6. Taylor McNeely
    7. Michael Leone
    8. Robert P Wersto
    9. Kathy A Perdue
    10. Isabel Beerman
    This article has been curated by 1 group:
    • Curated by eLife

      Evaluation Summary:

      Yanai et al. used flow cytometry and methlyation profiling to characterize populations of immune cells in the peripheral blood of male rats, finding age-dependent differences in cell composition and DNA methylation profiles, with marked changes occurring at specific time points (e.g., at 15 months and 24 months of age). This raises the possibility that interventions to modify blood aging may be most effective if done prior to these inflection points. This manuscript will be of broad interest to scientists in the geroscience realm and in particular to those using the aging rat as a model for the aging human hematopoietic system.

      (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

    Reviewed by eLife

    This article has 5 evaluationsAppears in 1 listLatest version Latest activity
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