Developmental Biology

Precise base editing for the in vivo study of developmental signaling and human pathologies in zebrafish

1. Marion Rosello
2. Juliette Vougny
3. François Czarny
4. Marina C Mione
5. Jean-Paul Concordet
6. Shahad Albadri
7. Filippo Del Bene

• Curated by eLife

  Evaluation Summary:

  The manuscript by Rosello et al. represents a major advance in implementation of cutting-edge genome editing methodologies in the zebrafish. The study seeks to describe optimized tools for precise base editing in zebrafish and to demonstrate their effective application. Overall, this study demonstrates that cytosine base editing is an efficient and powerful method for introducing precise in vivo edits into the zebrafish genome, and will be of interest to scientists who use zebrafish and other genetic systems to model human development and disease.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

Insulin-producing β-cells regenerate ectopically from a mesodermal origin under the perturbation of hemato-endothelial specification

1. Ka-Cheuk Liu
2. Alethia Villasenor
3. Maria Bertuzzi
4. Nicole Schmitner
5. Niki Radros
6. Linn Rautio
7. Kenny Mattonet
8. Ryota L Matsuoka
9. Sven Reischauer
10. Didier YR Stainier
11. Olov Andersson

• Curated by eLife

  Evaluation Summary:

  This is an elegant study demonstrating the emergence of mesoderm-derived beta-like cells following beta-cell ablation in an endothelial cell deficient context. These findings will be of interest to scientists in the areas of regeneration and reprogramming, as they reveal a previously unknown degree of germ layer plasticity in the embryo. In the long term the study has potential impact in the diabetes field, as it reveals a novel path for redirecting somatic cells into insulin-producing cells in an in vivo context.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #3 agreed to share their names with the authors.)

Reviewed by eLife

Glypicans specifically regulate Hedgehog signaling through their interaction with Ihog in cytonemes

1. Eleanor Simon
2. Carlos Jimenez-Jimenez
3. Irene Seijo-Barandiaran
4. Gustavo Aguilar
5. David Sanchez-Hernandez
6. Adrian Aguirre-Tamaral
7. Laura Gonzalez-Mendez
8. Pedro Ripoll
9. Isabel Guerrero

Reviewed by eLife

Combinatorial patterns of graded RhoA activation and uniform F-actin depletion promote tissue curvature

1. Marlis Denk-Lobnig
2. Jan F. Totz
3. Natalie C. Heer
4. Jörn Dunkel
5. Adam C. Martin

Reviewed by Review Commons, preLights

BAP1/ASXL complex modulation regulates epithelial-mesenchymal transition during trophoblast differentiation and invasion

1. Vicente Perez-Garcia
2. Georgia Lea
3. Pablo Lopez-Jimenez
4. Hanneke Okkenhaug
5. Graham J Burton
6. Ashley Moffett
7. Margherita Y Turco
8. Myriam Hemberger

Reviewed by eLife

Identification of Gli1 as a progenitor cell marker for meniscus development and injury repair

1. Yulong Wei
2. Hao Sun
3. Tao Gui
4. Lutian Yao
5. Leilei Zhong
6. Wei Yu
7. Su-Jin Heo
8. Lin Han
9. X. Sherry Liu
10. Yejia Zhang
11. Eiki Koyama
12. Fanxin Long
13. Miltiadis Zgonis
14. Robert L Mauck
15. Jaimo Ahn
16. Ling Qin

Reviewed by eLife

Beneficial impacts of neuromuscular electrical stimulation on muscle structure and function in the zebrafish model of Duchenne muscular dystrophy

1. Elisabeth A Kilroy
2. Amanda C Ignacz
3. Kaylee L Brann
4. Claire E Schaffer
5. Devon Varney
6. Sarah S Alrowaished
7. Kodey J Silknitter
8. Jordan N Miner
9. Ahmed Almaghasilah
10. Tashawna L Spellen
11. Alexandra D Lewis
12. Karissa Tilbury
13. Benjamin L King
14. Joshua B Kelley
15. Clarissa A Henry

• Curated by eLife

  Summary: The authors seek to tackle the question of exercise and inactivity in Duchenne muscular dystrophy, an important and unsolved issue. They use the zebrafish model system and two paradigms, one an inactivity paradigm (using tricaine) and the other an exercise paradigm using NMES. They find that inactivity worsens the dystrophic phenotype, and that different exercise paradigms impact the dystrophic phenotype differently. Overall this is an important study with exciting data and a potential to impact our understanding of exercise in DMD. However, as described below, all reviewers felt that several critical experimental considerations are necessary to consider in order to substantiate the data claims.

Reviewed by eLife

Glial Hedgehog signalling and lipid metabolism regulate neural stem cell proliferation in Drosophila

1. Qian Dong
2. Michael Zavortink
3. Francesca Froldi
4. Sofya Golenkina
5. Tammy Lam
6. Louise Y Cheng

Reviewed by Review Commons

TWIST1 and chromatin regulatory proteins interact to guide neural crest cell differentiation

1. Xiaochen Fan
2. V Pragathi Masamsetti
3. Jane QJ Sun
4. Kasper Engholm-Keller
5. Pierre Osteil
6. Joshua Studdert
7. Mark E Graham
8. Nicolas Fossat
9. Patrick PL Tam

Reviewed by eLife

NODAL/TGFβ signalling mediates the self-sustained stemness induced by PIK3CAH1047R homozygosity in pluripotent stem cells

1. Ralitsa R. Madsen
2. James Longden
3. Rachel G. Knox
4. Xavier Robin
5. Franziska Völlmy
6. Kenneth G. Macleod
7. Larissa S. Moniz
8. Neil O. Carragher
9. Rune Linding
10. Bart Vanhaesebroeck
11. Robert K. Semple

Reviewed by Review Commons