Developmental Biology

Proteolysis of fibrillin-2 microfibrils is essential for normal skeletal development

1. Timothy J Mead
2. Daniel R Martin
3. Lauren W Wang
4. Stuart A Cain
5. Cagri Gulec
6. Elisabeth Cahill
7. Joseph Mauch
8. Dieter Reinhardt
9. Cecilia Lo
10. Clair Baldock
11. Suneel S Apte

• Curated by eLife

  Evaluation Summary:

  The study by Mead and colleagues proves that the in vivo genetic interactions between Adamts 6 and fibrillin 2 is critical for normal endochondral bone development. In particular, the authors show that global loss of ADAMTS6 causes a severe chondrodysplasia that is significantly worsened by concomitant loss of ADAMTS10 and, conversely, almost fully prevented by haploinsufficiency for fibrillin 2, a substrate of ADAMTS6. The paper expands and deepens our current undertstanding of proteases and their substrates in endochondral bone development.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

A cellular and molecular atlas reveals the basis of chytrid development

1. Davis Laundon
2. Nathan Chrismas
3. Kimberley Bird
4. Seth Thomas
5. Thomas Mock
6. Michael Cunliffe

• Curated by eLife

  Evaluation Summary:

  This work provides transcriptional profiling and structural insight into chytrid development and will very likely stimulate further work on this model.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.

Reviewed by eLife

A cellular and molecular analysis of SoxB-driven neurogenesis in a cnidarian

1. Eleni Chrysostomou
2. Hakima Flici
3. Sebastian G Gornik
4. Miguel Salinas-Saavedra
5. James M Gahan
6. Emma T McMahon
7. Kerry Thompson
8. Shirley Hanley
9. Michelle Kilcoyne
10. Christine E Schnitzler
11. Paul Gonzalez
12. Andreas D Baxevanis
13. Uri Frank

Reviewed by Review Commons

A dysmorphic mouse model reveals developmental interactions of chondrocranium and dermatocranium

1. Susan M Motch Perrine
2. M Kathleen Pitirri
3. Emily L Durham
4. Mizuho Kawasaki
5. Hao Zheng
6. Danny Z Chen
7. Kazuhiko Kawasaki
8. Joan T Richtsmeier

• Curated by eLife

  Evaluation Summary:

  This work combines imaging and quantitative analyses to address the conclusions that the chondrocranium and dermatocranium form an integrated unit of development and alterations in the chondrocranium drive changes in the dermatocranium. However, the manuscript in the current form suffers from data uncertainty because raw data was not provided, and the title and the conclusions go too much beyond the results they have gathered.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Plexins promote Hedgehog signaling through their cytoplasmic GAP activity

1. Justine M Pinskey
2. Tyler M Hoard
3. Xiao-Feng Zhao
4. Nicole E Franks
5. Zoë C Frank
6. Alexandra N McMellen
7. Roman J Giger
8. Benjamin L Allen

• Curated by eLife

  Evaluation Summary:

  Prompted by previous findings that Neuropilins can stimulate activity of the Hh signalling pathway, the authors have investigated the possible involvement of Plexins in this process. Using NIH3T3 cells as an assay system, they present evidence that multiple Plexins are able to enhance the transcriptional response to SHH ligand and that this requires activity of the C-terminal intracellular domain of the Plexin protein. They present in vivo evidence that Plexins can stimulate and are required for HH-dependent processes in the CNS. The data are compelling and add further insights into the workings of this important signalling pathway.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife

Single-cell RNA-seq reveals dynamic transcriptome profiling in human early neural differentiation

1. Zhouchun Shang
2. Dongsheng Chen
3. Quanlei Wang
4. Shengpeng Wang
5. Qiuting Deng
6. Liang Wu
7. Chuanyu Liu
8. Xiangning Ding
9. Shiyou Wang
10. Jixing Zhong
11. Doudou Zhang
12. Xiaodong Cai
13. Shida Zhu
14. Huanming Yang
15. Longqi Liu
16. J Lynn Fink
17. Fang Chen
18. Xiaoqing Liu
19. Zhengliang Gao
20. Xun Xu

Reviewed by GigaScience

Inflammatory response in hematopoietic stem and progenitor cells triggered by activating SHP2 mutations evokes blood defects

1. Maja Solman
2. Sasja Blokzijl-Franke
3. Florian Piques
4. Chuan Yan
5. Qiqi Yang
6. Marion Strullu
7. Sarah M Kamel
8. Pakize Ak
9. Jeroen Bakkers
10. David M Langenau
11. Hélène Cavé
12. Jeroen den Hertog

• Curated by eLife

  Evaluation Summary:

  The authors of this paper model the D61G mutation in the gene PTPN11 that encodes the protein-tyrosine phosphatase SHP2 in zebrafish, creating a model consistent with the human Noonan syndrome (NS), which is predisposed to a juvenile myelomonocytic leukemia (JMML) and myeloproliferative neoplasm (MPN)-like syndrome. The study nicely provides a new model that can be used as the basis of future studies in the field. Because the mutant variably displays phenotypes along a spectrum from NS to MPN, different researchers can choose to focus on this as they see fit.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Wnt11 acts on dermomyotome cells to guide epaxial myotome morphogenesis

1. Ann Kathrin Heilig
2. Ryohei Nakamura
3. Atsuko Shimada
4. Yuka Hashimoto
5. Yuta Nakamura
6. Joachim Wittbrodt
7. Hiroyuki Takeda
8. Toru Kawanishi

• Curated by eLife

  Evaluation Summary:

  This study is of interest to researchers who study cell migration and or muscle development. It builds upon prior analysis of Double Anal fin (Da) mutants by using detailed bioinformatic and time-lapse analysis to explain dorsal somite extension and find evidence that dorsal muscle morphogenesis is actively guided, rather than being passively shaped by physical constraints alone. This work illustrates the dynamic behaviors of dorsal somitic cells, which form elaborate protrusions, delaminate from their parent somite, and bridge the gap between opposing epaxial myotomes. Looking downstream of Da, they show that Wnt signaling is central to dorsal extension of the epaxial myotome in medaka and propose that similar functions may shape the dorsal musculature across vertebrates.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

A regeneration-triggered metabolic adaptation is necessary for cell identity transitions and cell cycle re-entry to support blastema formation and bone regeneration

1. Ana S Brandão
2. Jorge Borbinha
3. Telmo Pereira
4. Patrícia H Brito
5. Raquel Lourenço
6. Anabela Bensimon-Brito
7. Antonio Jacinto

• Curated by eLife

  Evaluation Summary:

  The authors provide compelling evidence to show that injury induces activation of glycolysis during zebrafish adult tail fin regeneration. This early activation is crucial for osteoblast dedifferentiation and proliferation, which are required for blastema formation and tail fin regeneration. However, additional data are required to support the claim of a "metabolic switch" from oxidative phosphorylation to glycolysis. This study will be of interest to a broad audience in the fields of regeneration and metabolic regulation of developmental processes.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Lhx2 is a progenitor-intrinsic modulator of Sonic Hedgehog signaling during early retinal neurogenesis

1. Xiaodong Li
2. Patrick J Gordon
3. John A Gaynes
4. Alexandra W Fuller
5. Randy Ringuette
6. Clayton P Santiago
7. Valerie Wallace
8. Seth Blackshaw
9. Pulin Li
10. Edward M Levine

Reviewed by Review Commons