Developmental Biology

Incomplete activation of developmentally required genes Alyref1 and Gabpb1 leads to preimplantation arrest in cloned mouse embryos

1. Shunya Ihashi
2. Mizuto Hamanaka
3. Masaya Kaji
4. Miki Mori
5. Yuma Imasato
6. Misaki Nakamura
7. Masayuki Anzai
8. Kazuya Matsumoto
9. Masahito Ikawa
10. Kei Miyamoto

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Sociosexual behavior requires both activating and repressive roles of Tfap2e/AP-2ε in vomeronasal sensory neurons

1. Jennifer M Lin
2. Tyler A Mitchell
3. Megan Rothstein
4. Alison Pehl
5. Ed Zandro M Taroc
6. Raghu R Katreddi
7. Katherine E Parra
8. Damian G Zuloaga
9. Marcos Simoes-Costa
10. Paolo Emanuele Forni

• Curated by eLife

  Evaluation Summary:

  This paper studies how pheromone-sensing neurons of the vomeronasal organ establish and maintain cell identity. A role for a particular transcription factor, AP-2e, is investigated using targeted gene knockout and rescue, and observations reveal an unexpected plasticity in the cell identity of mature sensory neurons. This paper should be of broad interest to neuroscientists and cell biologists studying mechanisms of cell development and differentiation.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

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A tripartite structure, the complex nuclear receptor element (cNRE), is a c is-regulatory module of viral origin required for atrial chamber preferential gene expression

1. Luana Nunes Santos
2. Ângela Maria da Souza Costa
3. Martin Nikolov
4. Allysson Coelho Sampaio
5. Frank E. Stockdale
6. Gang F Wangø
7. Hozana Andrade Castillo
8. Mariana Bortoletto Grizante
9. Stefanie Dudczig
10. Michelle Vasconcelos
11. Nadia Rosenthal
12. Patricia Regina Jusuf
13. Paulo de Oliveira
14. Tatiana Guimarães de Freitas Matos
15. William Nikovits
16. Michael Schubert
17. Mirana Ramialison
18. José Xavier-Neto

• Curated by eLife

  Evaluation Summary:

  In this manuscript Nunes Santos et al. use a combination of computation and experimental methods to identify and characterize a cis-regulatory element that mediates expression of the quail Slow Myosin Heavy Chain III (SMyHC III) gene in the heart. The study contributes to our understanding of how genes can be expressed differentially in the atrial and ventricular chambers of the heart. The evidence for the newly-identified gene regulatory sequence, and its origin, in exclusively directing these gene expression differences could be stronger. This study is of potential interest to readers in the fields of developmental biology, evolution, gene regulation, and biology of repeats.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

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Src42A is required for E-cadherin dynamics at cell junctions during Drosophila axis elongation

1. Lenin Chandran
2. Wilko Backer
3. Raphael Schleutker
4. Deqing Kong
5. Seyed A. H. Beati
6. Stefan Luschnig
7. H.-Arno J. Müller

Reviewed by Review Commons

“Integration of multimodal data in the developing tooth reveals candidate dental disease genes”

1. Emma Wentworth Winchester
2. Alexis Hardy
3. Justin Cotney

• Curated by eLife

  Evaluation Summary:

  This study, which will be of interest to basic scientists and clinicians in the fields of dental and craniofacial malformations, explores the validity and usefulness of mouse teeth as a model for studying the role of enhancers in human dental development and disease, based on a multi-layered integrated analysis of genetic data with different available data from mice and humans. The results from the study can provide a useful tool for the manipulation of expression of reporter or other genes in a tooth- or enamel-specific manner.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

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A neurogenic signature involving monoamine Oxidase-A controls human thermogenic adipose tissue development

1. Javier Solivan-Rivera
2. Zinger Yang Loureiro
3. Tiffany DeSouza
4. Anand Desai
5. Sabine Pallat
6. Qin Yang
7. Raziel Rojas-Rodriguez
8. Rachel Ziegler
9. Pantos Skritakis
10. Shannon Joyce
11. Denise Zhong
12. Tammy Nguyen
13. Silvia Corvera

• Curated by eLife

  Evaluation Summary:

  This manuscript uses a species-hybrid model in which functional human white and thermogenic adipose tissues develop in mice. Interestingly, human adipose tissue is fully able to recruit mouse vascular networks and sympathetic innervation during human adipocyte development. These findings provide novel and valuable information about the development of human thermogenic adipose tissue.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

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C. elegans molting requires rhythmic accumulation of the Grainyhead/ LSF transcription factor GRH ‐1

1. Milou W M Meeuse
2. Yannick P Hauser
3. Smita Nahar
4. A Alexander T Smith
5. Kathrin Braun
6. Chiara Azzi
7. Markus Rempfler
8. Helge Großhans

Reviewed by Review Commons

The cardiopharyngeal mesoderm contributes to lymphatic vessel development in mouse

1. Kazuaki Maruyama
2. Sachiko Miyagawa-Tomita
3. Yuka Haneda
4. Mayuko Kida
5. Fumio Matsuzaki
6. Kyoko Imanaka-Yoshida
7. Hiroki Kurihara

Reviewed by Review Commons

Regionally distinct trophoblast regulate barrier function and invasion in the human placenta

1. Bryan Marsh
2. Yan Zhou
3. Mirhan Kapidzic
4. Susan Fisher
5. Robert Blelloch

• Curated by eLife

  Evaluation Summary:

  By using single-cell RNA sequencing, elegant computational approaches, protein validation, and in vitro functional assays, this study characterizes the cellular composition and gene expression profiles of the human placenta in mid-gestation. The findings and dataset provided by the authors represent an important resource for readers interested in human development and placenta biology. However, conclusions require additional experimental support.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

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TALPID3/KIAA0586 Regulates Multiple Aspects of Neuromuscular Patterning During Gastrointestinal Development in Animal Models and Human

1. Jean Marie Delalande
2. Nandor Nagy
3. Conor J. McCann
4. Dipa Natarajan
5. Julie E. Cooper
6. Gabriela Carreno
7. David Dora
8. Alison Campbell
9. Nicole Laurent
10. Polychronis Kemos
11. Sophie Thomas
12. Caroline Alby
13. Tania Attié-Bitach
14. Stanislas Lyonnet
15. Malcolm P. Logan
16. Allan M. Goldstein
17. Megan G. Davey
18. Robert M. W. Hofstra
19. Nikhil Thapar
20. Alan J. Burns

• Curated by eLife

  Evaluation Summary:

  In this study, the authors used a chick and a mouse model and human tissues to analyze the role of the conserved protein TALPID3/KIAA0586, previously linked to ciliogenesis, in gut development. Using a multi-species approach, the authors conclude that TALPID3 has an evolutionary conserved role in regulating gut patterning along the radial axis, apparently orchestrated by neural crest cells in a non-cell-autonomous manner and mediated by perturbation of Sonic Hedgehog signaling and the composition of extracellular matrix.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript.The reviewers remained anonymous to the authors.)

Reviewed by eLife