Developmental Biology

Complex aneuploidy triggers autophagy and p53-mediated apoptosis and impairs the second lineage segregation in human preimplantation embryos

1. Marius Regin
2. Yingnan Lei
3. Edouard Couvreu De Deckersberg
4. Charlotte Janssens
5. Anfien Huyghebaert
6. Yves Guns
7. Pieter Verdyck
8. Greta Verheyen
9. Hilde Van de Velde
10. Karen Sermon
11. Claudia Spits

• Curated by eLife

  eLife Assessment

  This study provides valuable insights into the cellular responses to complex aneuploidy in human preimplantation embryos. The evidence supporting the claims of the authors is now convincing after addressing previous concerns. This work will be of interest to embryologists, geneticists and scholars working on reproductive medicine by increasing our understanding of how human embryos respond to chromosomal abnormalities.

Reviewed by eLife

Inclusive, exclusive and hierarchical atlas of NFATc1+/PDGFR-α+ cells in dental and periodontal mesenchyme

1. Xue Yang
2. Chuyi Han
3. Changhao Yu
4. Bin Zhou
5. Ling Ye
6. Feifei Li
7. Fanyuan Yu

• Curated by eLife

  eLife Assessment

  Utilizing transgenic lineage tracing techniques and tissue clearing-based advanced imaging and three-dimensional slices reconstruction, the authors comprehensively mapped the distribution atlas of NFATc1+ and PDGFR-α+ cells in dental and periodontal mesenchyme and tracked their in vivo fate trajectories. This important work extends our understanding of NFATc1+ and PDGFR-α+ cells in dental and periodontal mesenchyme homeostasis, and should provide impact on clinical application and investigation. The strength of this work is compelling in employing CRISPR/Cas9-mediated gene editing to generate two dual recombination systems, and mapped gNFATc1+ and PDGFR-α+cells residing in dental and periodontal mesenchyme, their capacity for progeny cell generation, and their inclusive, exclusive and hierarchical relations in homeostasis, generating a spatiotemporal atlas of these skeletal stem cell population.

Reviewed by eLife

Flamingo participates in multiple models of cell competition

1. Pablo Sanchez Bosch
2. Bomsoo Cho
3. Jeffrey D Axelrod

• Curated by eLife

  eLife Assessment

  This study investigates the role of the Cadherin Flamingo (Fmi) in cell competition in developing tissues in Drosophila melanogaster. The findings are valuable in that they show that Fmi is required in winning cells in several competitive contexts. The evidence supporting the conclusions is solid, as the authors identify Fmi as a potential new regulator of cell competition, however, they don't delve into a mechanistic understanding of how this occurs.

Reviewed by eLife

In vivo transition in chromatin accessibility during differentiation of deep-layer excitatory neurons in the neocortex

1. Seishin Sakai
2. Yurie Maeda
3. Mai Saeki
4. Daijiro Konno
5. Keita Kawaji
6. Fumio Matsuzaki
7. Yutaka Suzuki
8. Yukiko Gotoh
9. Yusuke Kishi

Reviewed by Review Commons

Lineage-specific intersection of endothelin and GDNF signaling in enteric nervous system development

1. Denise M Poltavski
2. Alexander T Cunha
3. Jaime Tan
4. Henry M Sucov
5. Takako Makita

• Curated by eLife

  eLife Assessment

  This study provides valuable insights into our understanding of the development of the enteric nervous system. The authors use genetically engineered mice to study the behavior of stem cells in organizing the enteric nervous system and the secreted signals that regulate these cells. The study rests on a degree of incomplete evidence since the characterization of some of the mouse resources is not complete in the current version.

Reviewed by eLife

Identification of an early subset of cerebellar nuclei neurons in mice

1. Maryam Rahimi-Balaei
2. Shayan Amiri
3. Thomas Lamonerie
4. Sih-Rong Wu
5. Huda Y Zoghbi
6. G Giacomo Consalez
7. Daniel Goldowitz
8. Hassan Marzban

• Curated by eLife

  eLife Assessment

  The authors are interested in the developmental origin of the neurons of the cerebellar nuclei. In this study, they identify a population of neurons with a specific complement of markers that originate in a distinct location from where cerebellar nuclear precursor cells have been thought to originate that show distinct developmental properties. The discovery of a new germinal zone giving rise to a new population of neurons is an exciting finding, and it enriches our understanding of cerebellar development. The important claims, better explained in the current version, are well supported by solid evidence with the authors using a wide range of technical approaches, including transgenic mice that allow them to disentangle the influence of distinct developmental organizers

Reviewed by eLife

Maternal obesity may disrupt offspring metabolism by inducing oocyte genome hyper-methylation via increased DNMTs

1. Shuo Chao
2. Jun Lu
3. Li-Jun Li
4. Hong-Yan Guo
5. Kuipeng Xu
6. Ning Wang
7. Shu-Xian Zhao
8. Xiao-Wen Jin
9. Shao-Ge Wang
10. Shen Yin
11. Wei Shen
12. Ming-Hui Zhao
13. Gui-An Huang
14. Qing-Yuan Sun
15. Zhao-Jia Ge

• Curated by eLife

  eLife Assessment

  This manuscript reports important findings on the impact of maternal obesity on offspring metabolism. It presents solid evidence that maternal obesity induces genomic methylation alterations in oocytes, which can be partly transmitted to F2 in females, and that melatonin is involved in regulating the hyper-methylation of high fat diet oocytes by increasing the expression of DNMTs via the cAMP/PKA/CREB pathway. This study would be of interest to biologists in the fields of epigenetics and metabolism.

Reviewed by eLife

Single Cell Transcriptomics-Informed Induced Pluripotent Stem Cells Differentiation to Tenogenic Lineage

1. Angela Papalamprou
2. Victoria Yu
3. Wensen Jiang
4. Julia Sheyn
5. Tina Stefanovic
6. Angel Chen
7. Chloe Castaneda
8. Melissa Chavez
9. Dmitriy Sheyn

• Curated by eLife

  eLife Assessment

  The authors established a useful syndetome differentiation protocol from human induced pluripotent stem cells, guided by single-cell transcriptomic analysis. Their findings could significantly impact the field, particularly for patients needing tendon cell therapy. However, the evidence presented is currently incomplete, as the authors did not yet test the applicability of their protocol across multiple human induced pluripotent stem cell lines.

Reviewed by eLife

Basement membranes are crucial for proper olfactory placode shape, position and boundary with the brain, and for olfactory axon development

1. Pénélope Tignard
2. Karen Pottin
3. Audrey Geeverding
4. Mohamed Doulazmi
5. Mélody Cabrera
6. Coralie Fouquet
7. Mathilde Liffran
8. Jonathan Fouchard
9. Marion Rosello
10. Shahad Albadri
11. Filippo Del Bene
12. Alain Trembleau
13. Marie Anne Breau

• Curated by eLife

  eLife Assessment

  This important study describes the function of Laminin y1-dependent basement membranes in development of the olfactory placode, including morphogenesis of the placode, boundary formation, and olfactory axonal pathfinding. The study uses elegant live imaging approaches and extensive quantitative analyses, combined with detailed mutant analyses to provide a compelling description of the role of Laminin in olfactory placode development. In addition to the contributions this study makes to understanding olfactory placode development, it will also be of broader interest to individuals studying extracellular matrix regulation of tissue morphogenesis, and neural development including neuronal pathfinding.

Reviewed by eLife

Actin-based deformations of the nucleus control multiciliated ependymal cell differentiation

1. Marianne Basso
2. Alexia Mahuzier
3. Syed Kaabir Ali
4. Anaïs Marty
5. Marion Faucourt
6. Ana-Maria Lennon-Duménil
7. Ayush Srivastava
8. Michella Khoury Damaa
9. Alexia Bankole
10. Alice Meunier
11. Ayako Yamada
12. Julie Plastino
13. Nathalie Spassky
14. Nathalie Delgehyr

Reviewed by preLights, Arcadia Science