Cell Biology

Carm1-arginine methylation of the transcription factor C/EBPα regulates transdifferentiation velocity

1. Guillem Torcal Garcia
2. Elisabeth Kowenz-Leutz
3. Tian V Tian
4. Antonis Klonizakis
5. Jonathan Lerner
6. Luisa De Andres-Aguayo
7. Valeriia Sapozhnikova
8. Clara Berenguer
9. Marcos Plana Carmona
10. Maria Vila Casadesus
11. Romain Bulteau
12. Mirko Francesconi
13. Sandra Peiro
14. Philipp Mertins
15. Kenneth Zaret
16. Achim Leutz
17. Thomas Graf

• Curated by eLife

  eLife assessment

  This important manuscript describes how methylation of a single arginine residue in a transcription factor, C/EBPα, can alter dynamics of cell fate transition. The study provides one of the most striking examples of the transcription factor regulation by methylation and is well-executed, with compelling evidence to support the authors' claims.

Reviewed by eLife

Caveolae and Bin1 form ring-shaped platforms for T-tubule initiation

1. Eline Lemerle
2. Jeanne Lainé
3. Marion Benoist
4. Gilles Moulay
5. Anne Bigot
6. Clémence Labasse
7. Angéline Madelaine
8. Alexis Canette
9. Perrine Aubin
10. Jean-Michel Vallat
11. Norma B Romero
12. Marc Bitoun
13. Vincent Mouly
14. Isabelle Marty
15. Bruno Cadot
16. Laura Picas
17. Stéphane Vassilopoulos

• Curated by eLife

  eLife assessment

  Lemerle et al utilize advanced correlative light and electron microscopy and molecular biology approaches to convincingly demonstrate the presence of the membrane-bending protein Bin1 and caveolae containing rings capable of membrane tubulation in developing muscle. The data is highly significant as it potentially advances our fundamental understanding of how transverse tubules are formed, a significant gap in our understanding of excitation-contraction coupling and muscle biology more broadly.

Reviewed by eLife

Defining basic rules for hardening influenza A virus liquid condensates

1. Temitope Akhigbe Etibor
2. Silvia Vale-Costa
3. Sindhuja Sridharan
4. Daniela Brás
5. Isabelle Becher
6. Victor Hugo Mello
7. Filipe Ferreira
8. Marta Alenquer
9. Mikhail M Savitski
10. Maria-João Amorim

• Curated by eLife

  eLife assessment

  The work presented is fundamental to the field of negative-sense RNA viruses. It showcases a compelling set of ideas and approaches to study some physicochemical properties of organelles in live cells during a viral infection. However, the conclusions could benefit by adding a deeper theoretical approach and additional experimental support.

Reviewed by eLife

Creating an atlas of the bone microenvironment during oral inflammatory-related bone disease using single-cell profiling

1. Yi Fan
2. Ping Lyu
3. Ruiye Bi
4. Chen Cui
5. Ruoshi Xu
6. Clifford J Rosen
7. Quan Yuan
8. Chenchen Zhou

• Curated by eLife

  eLife assessment

  In this important project, the authors used single-cell RNA-sequencing (scRNA-Seq) technology to profile the transcriptome of alveolar bone marrow single cells and demonstrated the protective role of mesenchymal stem cells (MSCs) during apical periodontitis. With comprehensive data, the authors identified new inflammatory biomarkers associated with the pathogenesis of oral inflammatory diseases. Their study suggests that certain MSC subsets may have a potential role in healing bone lesions.

Reviewed by eLife

PI3P-dependent regulation of cell size and autophagy by phosphatidylinositol 5-phosphate 4-kinase

1. Avishek Ghosh
2. Aishwarya Venugopal
3. Dhananjay Shinde
4. Sanjeev Sharma
5. Meera Krishnan
6. Swarna Mathre
7. Harini Krishnan
8. Sankhanil Saha
9. Padinjat Raghu

Reviewed by Review Commons

Csf1 from marrow adipogenic precursors is required for osteoclast formation and hematopoiesis in bone

1. Leilei Zhong
2. Jiawei Lu
3. Jiankang Fang
4. Lutian Yao
5. Wei Yu
6. Tao Gui
7. Michael Duffy
8. Nicholas Holdreith
9. Catherine A Bautista
10. Xiaobin Huang
11. Shovik Bandyopadhyay
12. Kai Tan
13. Chider Chen
14. Yongwon Choi
15. Jean X Jiang
16. Shuying Yang
17. Wei Tong
18. Nathanial Dyment
19. Ling Qin

• Curated by eLife

  eLife assessment

  This paper is of potential interest for scientists who study bone marrow stem/progenitor cells, bone remodeling, and metabolism. Using Adipoq-Cre-based conditional deletion of Csf1 and scRNA-seq approaches, the authors provide compelling evidence that a subset of cells in murine bone marrow, characterized by AdipoQ expression, are a major source of M-CSF to regulate osteoclast formation and bone remodeling. This is a well-written, well-executed set of studies, the data from which largely support the above key claim.

Reviewed by eLife

A systematic approach to study protein-substrate specificity enables the identification of Ssh1 substrate range

1. Nir Cohen
2. Naama Aviram
3. Maya Schuldiner

Reviewed by Review Commons

MUC13 negatively regulates tight junction proteins and intestinal epithelial barrier integrity via protein kinase C

1. Celia Segui-Perez
2. Daphne A. C. Stapels
3. Ziliang Ma
4. Jinyi Su
5. Elsemieke Passchier
6. Bart Westendorp
7. Richard W. Wubbolts
8. Wei Wu
9. Jos P. M. van Putten
10. Karin Strijbis

Reviewed by Review Commons

An Sfi1-like centrin-interacting centriolar plaque protein affects nuclear microtubule homeostasis

1. Christoph Wenz
2. Caroline S. Simon
3. Tatiany Patricia Romão
4. Vanessa Stürmer
5. Marta Machado
6. Natacha Klages
7. Anja Klemmer
8. Yannik Voß
9. Markus Ganter
10. Mathieu Brochet
11. Julien Guizetti

Reviewed by Review Commons

LGG-1/GABARAP lipidation is not required for autophagy and development in Caenorhabditis elegans

1. Romane Leboutet
2. Céline Largeau
3. Leonie Müller
4. Magali Prigent
5. Grégoire Quinet
6. Manuel S Rodriguez
7. Marie-Hélène Cuif
8. Thorsten Hoppe
9. Emmanuel Culetto
10. Christophe Lefebvre
11. Renaud Legouis

Reviewed by Review Commons