Cell Biology

A TOPBP1 allele causing male infertility uncouples XY silencing dynamics from sex body formation

1. Carolline Ascenção
2. Jennie R Sims
3. Alexis Dziubek
4. William Comstock
5. Elizabeth A Fogarty
6. Jumana Badar
7. Raimundo Freire
8. Andrew Grimson
9. Robert S Weiss
10. Paula E Cohen
11. Marcus B Smolka

• Curated by eLife

  eLife assessment

  This important study reports a new mutant mouse line with compromised function of a DNA damage response protein. The evidence supporting the conclusion that the mutants display defective maintenance of meiotic sex chromosome inactivation is solid. This work is of interest to biomedical researchers working on meiosis and meiotic sex chromosome inactivation.

Reviewed by eLife

Organelle landscape analysis using a multi-parametric particle-based method

1. Yoshitaka Kurikawa
2. Ikuko Koyama-Honda
3. Keiko Igarashi
4. Norito Tamura
5. Seiichi Koike
6. Noboru Mizushima

Reviewed by Review Commons

Calcium transients trigger switch-like discharge of prostaglandin E2 in an extracellular signal-regulated kinase-dependent manner

1. Tetsuya Watabe
2. Shinya Yamahira
3. Kanako Takakura
4. Dean Thumkeo
5. Shuh Narumiya
6. Michiyuki Matsuda
7. Kenta Terai

• Curated by eLife

  eLife assessment

  This important study reports on the dynamics of PKA investigated at the single-cell level in vitro and in epithelia in vivo. Using different fluorescent biosensors and optogenetic actuators, the authors dissect the signaling pathway responsible for PKA waves, finding that PKA activation is a consequence of PGE2 release, which in turn is triggered by calcium pulses, requiring high ERK activity. The evidence supporting the claims is solid. At this stage the work is still partly descriptive in nature, and additional measurements would increase the strength of mechanistic insights and physiological relevance.

Reviewed by eLife

Surface curvature and basal hydraulic stress induce spatial bias in cell extrusion

1. Cheng-Kuang Huang
2. Xianbin Yong
3. David T. She
4. Chwee Teck Lim

• Curated by eLife

  eLife assessment

  This paper presents important findings into the response of epithelial monolayers to the combined effects of surface curvature and hydraulic stress, offering insights into how these cues contribute to epithelial cell extrusion. Most of the evidence is convincing, relying mainly on a combination of imaging-based techniques. This paper is of interest to a broad and growing community of biologists, biophysicists, and engineers interested in cell-geometry interactions.

Reviewed by eLife

ADPKD-Causing Missense Variants in Polycystin-1 Disrupt Cell Surface Localization or Polycystin Channel Function

1. Kotdaji Ha
2. Gabriel B. Loeb
3. Meyeon Park
4. Mohona Gupta
5. Yukako Akiyama
6. Jillian Argiris
7. Aide Pinedo
8. Christine Haewon Park
9. Nadav Brandes
10. F. Ritu
11. Chun Jimmie Ye
12. Jeremy F. Reiter
13. Markus Delling

Reviewed by Arcadia Science

Heparan sulfate promotes TRAIL-induced tumor cell apoptosis

1. Yin Luo
2. Huanmeng Hao
3. Zhangjie Wang
4. Chih Yean Ong
5. Robert Dutcher
6. Yongmei Xu
7. Jian Liu
8. Lars C Pedersen
9. Ding Xu

• Curated by eLife

  eLife assessment

  This fundamental study advances our understanding of TRAIL-induced apoptosis by defining how Heparan triggers this pathway at the molecular level. The evidence supporting the conclusions is compelling, with rigorous binding assays, structural methods, and cellular studies. The work will be of broad interest to cell biologists and biochemists.

Reviewed by eLife

Context-dependent modification of PFKFB3 in hematopoietic stem cells promotes anaerobic glycolysis and ensures stress hematopoiesis

1. Shintaro Watanuki
2. Hiroshi Kobayashi
3. Yuki Sugiura
4. Masamichi Yamamoto
5. Daiki Karigane
6. Kohei Shiroshita
7. Yuriko Sorimachi
8. Shinya Fujita
9. Takayuki Morikawa
10. Shuhei Koide
11. Motohiko Oshima
12. Akira Nishiyama
13. Koichi Murakami
14. Miho Haraguchi
15. Shinpei Tamaki
16. Takehiro Yamamoto
17. Tomohiro Yabushita
18. Yosuke Tanaka
19. Go Nagamatsu
20. Hiroaki Honda
21. Shinichiro Okamoto
22. Nobuhito Goda
23. Tomohiko Tamura
24. Ayako Nakamura-Ishizu
25. Makoto Suematsu
26. Atsushi Iwama
27. Toshio Suda
28. Keiyo Takubo

• Curated by eLife

  eLife assessment

  This important study provides novel strategies to overcome certain limitations when investigating the metabolism of hematopoietic stem cells, mainly due to their low abundance. The study provides compelling evidence suggesting that proliferative hematopoietic stem cells mainly use glycolysis (rather than mitochondrial OXPHOS or TCA cycle) as their primary energy source during emergency hematopoiesis. The article provides direct links between metabolic features and cell proliferation and explores alternative energy sources, and is of great interest to stem cell biologists.

Reviewed by eLife

Basement membrane diversification relies on two competitive secretory routes defined by Rab10 and Rab8 and modulated by dystrophin and the exocyst complex

1. Cynthia Dennis
2. Pierre Pouchin
3. Graziella Richard
4. Vincent Mirouse

Reviewed by Review Commons

Post-transcriptional splicing can occur in a slow-moving zone around the gene

1. Allison Coté
2. Aoife O'Farrell
3. Ian Dardani
4. Margaret Dunagin
5. Chris Coté
6. Yihan Wan
7. Sareh Bayatpour
8. Heather L Drexler
9. Katherine A Alexander
10. Fei Chen
11. Asmamaw T Wassie
12. Rohan Patel
13. Kenneth Pham
14. Edward S Boyden
15. Shelly Berger
16. Jennifer Phillips-Cremins
17. L Stirling Churchman
18. Arjun Raj

• Curated by eLife

  eLife assessment

  This fundamental study addresses a long-standing mystery in splicing regulation: does splicing occur co- or post-transcriptionally? The authors provide compelling evidence demonstrating that splicing can occur post-transcriptionally at a transcription site proximal zone, changing the way we think about splicing.

Reviewed by eLife

Arp2/3-dependent endocytosis ensures Cdc42 oscillations by removing Pak1-mediated negative feedback

1. Marcus A. Harrell
2. Ziyi Liu
3. Bethany F. Campbell
4. Olivia Chinsen
5. Tian Hong
6. Maitreyi Das

Reviewed by Arcadia Science