Cell Biology

Integrative vectors for regulated expression of SARS-CoV-2 proteins implicated in RNA metabolism

1. Stefan Bresson
2. Nic Robertson
3. Emanuela Sani
4. Tomasz W Turowski
5. Vadim Shchepachev
6. Michaela Kompauerova
7. Christos Spanos
8. Aleksandra Helwak
9. David Tollervey

Reviewed by ScreenIT

The Type 2 Asthma Mediator IL-13 Inhibits Severe Acute Respiratory Syndrome Coronavirus 2 Infection of Bronchial Epithelium

1. Luke R. Bonser
2. Walter L. Eckalbar
3. Lauren Rodriguez
4. Jiangshan Shen
5. Kyung Duk Koh
6. Khadija Ghias
7. Lorna T. Zlock
8. Stephanie Christenson
9. Prescott G. Woodruff
10. Walter E. Finkbeiner
11. David J. Erle

Reviewed by ScreenIT

A signal capture and proofreading mechanism for the KDEL-receptor explains selectivity and dynamic range in ER retrieval

1. Andreas Gerondopoulos
2. Philipp Bräuer
3. Tomoaki Sobajima
4. Zhiyi Wu
5. Joanne L Parker
6. Philip C Biggin
7. Francis A Barr
8. Simon Newstead

• Curated by eLife

  Evaluation Summary:

  Binding of cargo to sorting receptors in membrane trafficking is essential to cellular organization. This work is significant because it generates a detailed model of the key residues accounting for specificity and affinity of binding by the KDEL receptor. Interestingly, it is not the affinity per se that accounts for the specificity of cargo binding but rather charge-based exclusion of potentially competing signals.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Co-chaperone involvement in knob biogenesis implicates host-derived chaperones in malaria virulence

1. Mathias Diehl
2. Lena Roling
3. Lukas Rohland
4. Sebastian Weber
5. Marek Cyrklaff
6. Cecilia P. Sanchez
7. Carlo A. Beretta
8. Caroline S. Simon
9. Julien Guizetti
10. Julia Hahn
11. Norma Schulz
12. Matthias P. Mayer
13. Jude M. Przyborski

Reviewed by Review Commons

Quantitative mapping of human hair greying and reversal in relation to life stress

1. Ayelet M Rosenberg
2. Shannon Rausser
3. Junting Ren
4. Eugene V Mosharov
5. Gabriel Sturm
6. R Todd Ogden
7. Purvi Patel
8. Rajesh Kumar Soni
9. Clay Lacefield
10. Desmond J Tobin
11. Ralf Paus
12. Martin Picard

• Curated by eLife

  Evaluation Summary:

  This is an interesting and informative study reporting on the molecular features of reversible hair graying in humans and the connection with psychological stress. The study appears to have been very well conducted and the interpretations are generally supported by the data. While the results are primarily correlative at this stage, this work will set the stage for future more mechanistic studies and represents an important conceptual and methodological advance.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife, preLights

Ferroptotic stress promotes the accumulation of pro-inflammatory proximal tubular cells in maladaptive renal repair

1. Shintaro Ide
2. Yoshihiko Kobayashi
3. Kana Ide
4. Sarah A Strausser
5. Koki Abe
6. Savannah Herbek
7. Lori L O'Brien
8. Steven D Crowley
9. Laura Barisoni
10. Aleksandra Tata
11. Purushothama Rao Tata
12. Tomokazu Souma

• Curated by eLife

  Evaluation Summary:

  Ferroptotic cell death underlies tissue dysfunction inflicted by transient ischemia/reperfusion particularly in renal tissue. Here, the authors provide experimental evidence in animal models and human biopsies that mild and severe ischemic stress trigger distinctive cellular responses in proximal tubular cells which decide upon whether or not tissue may regenerate or fail. This is further corroborated in a genetic mouse model with mild ischemic stress-induced ablation of the key ferroptosis regulator glutathione peroxidase 4 (GPX4). These studies will be of significant interest both to those studying acute kidney injury and others interested in ischemic injury in other organ systems.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

SIRT1 regulates sphingolipid metabolism and neural differentiation of mouse embryonic stem cells through c-Myc-SMPDL3B

1. Wei Fan
2. Shuang Tang
3. Xiaojuan Fan
4. Yi Fang
5. Xiaojiang Xu
6. Leping Li
7. Jian Xu
8. Jian-Liang Li
9. Zefeng Wang
10. Xiaoling Li

• Curated by eLife

  Evaluation Summary:

  This paper will be of broad interest to cell biologists and advances the current understanding of the connection between lipid metabolism and stem cell function. The data generated from multiple complementary experimental approaches are of high quality and convincingly support the claims made.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

LIN37-DREAM prevents DNA end resection and homologous recombination at DNA double-strand breaks in quiescent cells

1. Bo-Ruei Chen
2. Yinan Wang
3. Anthony Tubbs
4. Dali Zong
5. Faith C Fowler
6. Nicholas Zolnerowich
7. Wei Wu
8. Amelia Bennett
9. Chun-Chin Chen
10. Wendy Feng
11. Andre Nussenzweig
12. Jessica K Tyler
13. Barry P Sleckman

• Curated by eLife

  Evaluation summary:

  This manuscript will be of interest for scientists interested in cell cycle, DNA repair, transcription and genome stability opening a new chapter in studies of cell cycle dependent regulation of DSB repair. Much of the prior work has focused on cell cycle-driven post-translational regulatory modification of DSB end resection, whereas the current work finds transcriptional programs are equally, if not more, important in controlling resection in G0. This could open possibilities for gene therapy in post-mitotic tissues. The data are of high quality and the conclusions drawn are supported by the experimental evidence.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

A phosphorylation of RIPK3 kinase initiates an intracellular apoptotic pathway that promotes prostaglandin2α-induced corpus luteum regression

1. Dianrong Li
2. Jie Chen
3. Jia Guo
4. Lin Li
5. Gaihong Cai
6. She Chen
7. Jia Huang
8. Hui Yang
9. Yinhua Zhuang
10. Fengchao Wang
11. Xiaodong Wang

• Curated by eLife

  Evaluation Summary:

  This manuscript is of potential interest to the field of cell death research in terms of understanding basic mechanisms and in the context of disease. The authors have used a broad range of methodologies and identified key phosphorylation sites on the protein kinase RIPK3 that determine whether cells undergo necroptotic or apoptotic cell death. The authors examine this phosphorylation event in the context of corpus luteum regression.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Quantitative mapping of dense microtubule arrays in mammalian neurons

1. Eugene A Katrukha
2. Daphne Jurriens
3. Desiree M Salas Pastene
4. Lukas C Kapitein

• Curated by eLife

  Evaluation Summary:

  In this manuscript, Katrukha et al. use STED and Expansion Microscopy techniques to map the distribution of different microtubule populations within the dendrites of neurons, a challenging task due to the tight bundling of microtubules along neuronal processes. They are able to show that dendritic microtubules are either acetylated or tyrosinated, but rarely have both or neither of these post-translational modifications. The strength of this paper is the quality of the experiments, the thoroughness of the analyses, and most importantly the transparent and critical discussion of the limitations the authors have encountered. This manuscript is of broad interest to the cytoskeletal and neurobiology fields.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #3 agreed to share their name with the authors.)

Reviewed by eLife