Cell Biology

Adult Stem Cell-derived Complete Lung Organoid Models Emulate Lung Disease in COVID-19

1. Courtney Tindle
2. MacKenzie Fuller
3. Ayden Fonseca
4. Sahar Taheri
5. Stella-Rita Ibeawuchi
6. Nathan Beutler
7. Gajanan D. Katkar
8. Amanraj Claire
9. Vanessa Castillo
10. Moises Hernandez
11. Hana Russo
12. Jason Duran
13. Laura E. Crotty Alexander
14. Ann Tipps
15. Grace Lin
16. Patricia A. Thistlethwaite
17. Ranajoy Chattopadhyay
18. Thomas F. Rogers
19. Debashis Sahoo
20. Pradipta Ghosh
21. Soumita Das

• Curated by eLife

  Evaluation Summary:

  The manuscript by Tindle et al describes generation of adult lung organoids (ALO) from human lung biopsies and their use to study the changes in gene expression as a result of SARS-CoV-2 infection. In this particular case the authors report the presence of AT1, AT2 cells, Basal cells, Goblet cells, Ciliated cells and Club cells. The authors were able to cultivate the cells at the air-liquid interface and to establish cultures of predominately proximal and predominately distal airway cells. The main finding is that proximal cells are more prone to viral infection, while distal cells are governing the exuberant inflammatory response, with both cells required for the exuberant response to occur. Useful information provided by the paper is the analysis gene signatures of various cellular models, in comparison to the infected human lung.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife, ScreenIT

CTP and parS coordinate ParB partition complex dynamics and ParA-ATPase activation for ParABS-mediated DNA partitioning

1. James A Taylor
2. Yeonee Seol
3. Jagat Budhathoki
4. Keir C Neuman
5. Kiyoshi Mizuuchi

• Curated by eLife

  Evaluation Summary:

  This manuscript reports carefully executed experiments on the dynamics of ParA-ParB and ParB-ParB interactions. Two main findings are presented: a change in stoichiometry of ParA-ParB interactions upon ligand binding and ligand dependent DNA condensation by ParB. The work is solid, the conclusions are generally well supported by the data, however, the relevance of some of the findings could be established more directly.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Single-cell transcriptomics defines heterogeneity of epicardial cells and fibroblasts within the infarcted heart

1. Julia Hesse
2. Christoph Owenier
3. Tobias Lautwein
4. Ria Zalfen
5. Jonas F. Weber
6. Zhaoping Ding
7. Christina Alter
8. Alexander Lang
9. Maria Grandoch
10. Norbert Gerdes
11. Jens W. Fischer
12. Gunnar W. Klau
13. Christoph Dieterich
14. Karl Köhrer
15. Jürgen Schrader

• Curated by eLife

  Evaluation Summary:

  The article by Hesse et al. defines heterogeneity of epicardial cells and fibroblasts in a murine model of cardiac injury to analyze the resulting populations through single cell RNA sequencing. Spatial confirmation of associated markers is performed using in-situ RNA hybridization. The work provides new insights into the heterogeneity of epicardial stromal and activated cardiac stromal cells post-injury.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Misoprostol Treatment Prevents Hypoxia-Induced Cardiac Dysfunction Through a 14-3-3 and PKA regulatory motif on Bnip3

1. Matthew D. Martens
2. Nivedita Seshadri
3. Lucas Nguyen
4. Donald Chapman
5. Elizabeth S. Henson
6. Bo Xiang
7. Landon Falk
8. Arielys Mendoza
9. Sunil Rattan
10. Spencer B. Gibson
11. Richard Keijzer
12. Ayesha Saleem
13. Grant M. Hatch
14. Christine A. Doucette
15. Jason M. Karch
16. Vernon W. Dolinsky
17. Ian M. Dixon
18. Adrian R. West
19. Christof Rampitsch
20. Joseph W. Gordon

Reviewed by Review Commons

HDLs extract lipophilic drugs from cells

1. Adi Zheng
2. Gilles Dubuis
3. Carla Susana Mendes Ferreira
4. Thomas Mercier
5. Laurent Decosterd
6. Christian Widmann

Reviewed by Review Commons

Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish

1. Meredith H Wilson
2. Stephen C Ekker
3. Steven A Farber

• Curated by eLife

  Evaluation Summary:

  This manuscript has generated novel and useful tools to mark cytoplasmic lipid droplets and monitor their dynamics in various tissues in live animals. It will be of interest to researchers studying lipid metabolism and related human diseases.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Augmin deficiency in neural stem cells causes p53-dependent apoptosis and aborts brain development

1. Ricardo Viais
2. Marcos Fariña-Mosquera
3. Marina Villamor-Payà
4. Sadanori Watanabe
5. Lluís Palenzuela
6. Cristina Lacasa
7. Jens Lüders

Reviewed by Review Commons

Latrophilin GPCR Signaling Mediates Synapse Formation

1. Richard Sando
2. Thomas C. Südhof

• Curated by eLife

  Evaluation Summary:

  The main finding that GPCR activity is necessary for latrophilins' role in synapse formation is both surprising and important. This work will inspire new research on compartmentalized GPCR signaling at the synapse.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

The ATF6β-calreticulin axis promotes neuronal survival under endoplasmic reticulum stress and excitotoxicity

1. Dinh Thi Nguyen
2. Thuong Manh Le
3. Tsuyoshi Hattori
4. Mika Takarada-Iemata
5. Hiroshi Ishii
6. Jureepon Roboon
7. Takashi Tamatani
8. Takayuki Kannon
9. Kazuyoshi Hosomichi
10. Atsushi Tajima
11. Shusuke Taniuchi
12. Masato Miyake
13. Seiichi Oyadomari
14. Shunsuke Saito
15. Kazutoshi Mori
16. Osamu Hori

Reviewed by Review Commons

Neuronal regulated ire-1-dependent mRNA decay controls germline differentiation in Caenorhabditis elegans

1. Mor Levi-Ferber
2. Rewayd Shalash
3. Adrien Le-Thomas
4. Yehuda Salzberg
5. Maor Shurgi
6. Jennifer IC Benichou
7. Avi Ashkenazi
8. Sivan Henis-Korenblit

• Curated by eLife

  Evaluation Summary:

  The authors of this manuscript previously showed that ER stress, and in particular the ER stress sensor Ire1, regulates transdifferentiation in C. elegans, leading to the ectopic differentiation of germline cells. In this follow-up manuscript, the authors present several lines of evidence supporting the idea that Ire1 modules these effects through degrading a novel mRNA substrate flp6. The authors identify the neurons and neuromodulators that affect accumulation of abnormal germline cells. The reviewers agreed that the discovery that flp6 is a regulated Ire-1-dependent decay target in C. elegans, and the demonstration of a non-cell-autonomous effect of Ire1 activity, are novel and likely to be of interest to a broad readership. However, more evidence is required to support some of the main conclusions.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife