Cell Biology

Nuclear Argonaute protein NRDE-3 switches small RNA binding partners during embryogenesis coincident with the formation of SIMR granules

1. Shihui Chen
2. Carolyn M Phillips

• Curated by eLife

  eLife Assessment

  The study by Chen and Phillips provides evidence for a dynamic switch in the small RNA repertoire of the Argonaute protein NRDE-3 during embryogenesis in C. elegans. The work is supported by solid experimental data, although some conclusions regarding the functional role of specific RNA granules remain uncertain. Nevertheless, this study offers valuable insights into RNA regulation and developmental biology, with broader implications for understanding small RNA pathways in other systems.

Reviewed by eLife

Membrane potential modulates ERK activity and cell proliferation

1. Mari Sasaki
2. Masanobu Nakahara
3. Takuya Hashiguchi
4. Fumihito Ono

• Curated by eLife

  eLife Assessment

  This important paper employs multiple experimental approaches and presents evidence that changes in membrane voltage directly affect ERK signaling to regulate cell division. This result is relevant because it supports an ion channel-independent pathway by which changes in membrane voltage can affect cell growth. The reviewers point out that some experimental results and interpretations are compelling, but the strength of evidence is incomplete and additional experiments are needed to rule out other possible interpretations of the data.

Reviewed by eLife

Intracellular diffusion in the cytoplasm increases with cell size in fission yeast

1. Catherine Tan
2. Michael C. Lanz
3. Matthew Swaffer
4. Jan Skotheim
5. Fred Chang

Reviewed by preLights

PKA regulation of neuronal function requires the dissociation of catalytic subunits from regulatory subunits

1. Wei-Hong Xiong
2. Maozhen Qin
3. Haining Zhong

• Curated by eLife

  eLife Assessment

  This important paper demonstrates that different PKA subtypes exhibit distinct subcellular localization at rest in CA1 neurons. The authors provide compelling evidence that when all tested PKA subtypes are activated by norepinephrine, catalytic subunits translocate to dendritic spines but regulatory subunits remain unmoved. Furthermore, PKA-dependent regulation of synaptic plasticity and transmission can be supported only by wildtype, dissociable PKA, but not by inseparable PKA.

Reviewed by eLife

Spatiotemporal recruitment of the ubiquitin-specific protease USP8 directs endosome maturation

1. Yue Miao
2. Yongtao Du
3. Baolei Wang
4. Jingjing Liang
5. Yu Liang
6. Song Dang
7. Jiahao Liu
8. Dong Li
9. Kangmin He
10. Mei Ding

• Curated by eLife

  eLife Assessment

  The manuscript presents an important model for the field of endosome maturation, providing perspective on the role of the deubiquitinating enzyme UPS-50/USP8 in the process. The evidence presented in the paper is clear, incorporating well-designed experiments that suggest the dual actions of UPS-50 and USP8 in the conversion of early endosomes into late endosomes. Overall, the work is convincing and centers on an intriguing subject.

Reviewed by eLife

Amoeboid cells undergo durotaxis with soft end polarized NMIIA

1. Chenlu Kang
2. Pengcheng Chen
3. Xin Yi
4. Dong Li
5. Yiping Hu
6. Yihong Yang
7. Huaqing Cai
8. Bo Li
9. Congying Wu

• Curated by eLife

  eLife Assessment

  This study presents an important finding on durotaxis in various amoeboid cells that is independent of focal adhesions. The evidence supporting the authors' claims is compelling. The work will be of interest to cell biologists and biophysicists working on rigidity sensing, the cytoskeleton, and cell migration.

Reviewed by eLife

Secreted exosomes induce filopodia formation

1. Caitlin McAtee
2. Mikin Patel
3. Daisuke Hoshino
4. Bong Hwan Sung
5. Ariana von Lersner
6. Mingjian Shi
7. Nan Hyung Hong
8. Anna Young
9. Evan Krystofiak
10. Andries Zijlstra
11. Alissa M Weaver

• Curated by eLife

  eLife Assessment

  The authors identify a novel relationship between exosome secretion and filopodia formation that has implications for cancer cell metastasis and neuronal synapse formation. Further, they identify the exosomal cargo, THSD7A, as a regulator of this process. The data presented is convincing, and represents an important advancement in our understanding of how these two biological processes are linked and play roles in regulating cell migration and cell-cell communication.

Reviewed by eLife

Non-disruptive inducible labeling of ER-membrane contact sites using the Lamin B Receptor

1. Laura Downie
2. Nuria Ferrandiz
3. Megan Jones
4. Stephen J. Royle

Reviewed by preLights

Golgi compaction facilitates microtubule nucleation to drive adult vertebrate peripheral neuron regeneration

1. Alice E Mortimer
2. Adam J Reid
3. Raman M Das

Reviewed by preLights

Single-nucleus transcriptomics reveal the cytological mechanism of conjugated linoleic acids in regulating intramuscular fat deposition

1. Liyi Wang
2. Shiqi Liu
3. Shu Zhang
4. Yizhen Wang
5. Yanbing Zhou
6. Tizhong Shan

• Curated by eLife

  eLife Assessment

  This revised study provides valuable information on the single nucleus RNA sequencing transcriptome, pathways, and cell types in pig skeletal muscle in response to conjugated linoleic acid (CLA) supplementation. Based on the comprehensive data analyses, the data are considered compelling and provide new insight into the mechanisms underlying intramuscular fat deposition and muscle fiber remodeling. The revised study clarifies major aspects of its methodology and analysis, addresses previous reviewer concerns, and contributes significantly to the understanding of nutritional strategies for fat infiltration in pig muscle.

Reviewed by eLife