Cancer Biology

Intravital deep-tumor single-beam 3-photon, 4-photon, and harmonic microscopy

1. Gert-Jan Bakker
2. Sarah Weischer
3. Júlia Ferrer Ortas
4. Judith Heidelin
5. Volker Andresen
6. Marcus Beutler
7. Emmanuel Beaurepaire
8. Peter Friedl

• Curated by eLife

  Summary: Nonlinear microscopy is in the unique position that high-resolution images of cells and other tissue components can be obtained in live tissue. However, scattering and absorption limit the penetration depth. The impact of nonlinear microscopy in biomedicine and biology would be much improved if higher imaging depths can be achieved. In this manuscript, the authors show they can accomplish imaging in complex specimens using 3- and 4-photon excitation, deeper in the specimen than comparable optics can accomplish with 2-photon excitation laser scanning microscopy. Using a customised commercial system, the authors have incorporated a high-powered laser source with an OPA and dispersion compensation to generate either 1330nm or 1650nm laser lines with high peak pulse energies at low pulse repetition rates. They then compare the relative capabilities of each laser line in terms of number of fluorescence emission channels measured (skin tumour xenografts), fluorescence bleaching analysis and functional toxicity thresholds and fluorescence signal attenuation (excised murine bone).

  This is a very interesting study with some potentially important findings from a technical perspective. However, there is a disconnect at present between the quality of the work and the quality of the presentation. There are many areas of quantitative imaging and intravital imaging that are well known to those in the direct field, and that are a complete mystery to the vast majority of those that are not. It would therefore be highly beneficial to restructure the manuscript in such a way that the findings can reach the many researchers that could benefit from this powerful approach rather than the few who already use it.

Reviewed by eLife

Convergent organization of aberrant MYB complex controls oncogenic gene expression in acute myeloid leukemia

1. Sumiko Takao
2. Lauren Forbes
3. Masahiro Uni
4. Shuyuan Cheng
5. Jose Mario Bello Pineda
6. Yusuke Tarumoto
7. Paolo Cifani
8. Gerard Minuesa
9. Celine Chen
10. Michael G. Kharas
11. Robert K. Bradley
12. Christopher R. Vakoc
13. Richard P. Koche
14. Alex Kentsis

Reviewed by Review Commons

Gene expression has more power for predicting in vitro cancer cell vulnerabilities than genomics

1. Joshua M. Dempster
2. John M. Krill-Burger
3. James M. McFarland
4. Allison Warren
5. Jesse S. Boehm
6. Francisca Vazquez
7. William C. Hahn
8. Todd R. Golub
9. Aviad Tsherniak

• Curated by eLife

  Summary: The authors propose a new approach to the derivation of cancer signatures and compare the relative impact of gene expression data with respect to other variables, particularly SVN and CVNs. The simplicity of the idea and of the technical approach, to the point of singling out a single gene predictive value, is a positive aspect. There are also critical aspects that will require substantial revision including the underlying influence of tissue specific genes. Overall, the paper provides a good basis for the generation of specific hypotheses that can be followed by additional validation studies at the computational and/or experimental level.

Reviewed by eLife

Tumor-derived extracellular vesicles regulate tumor-infiltrating regulatory T cells via the inhibitory immunoreceptor CD300a

1. Yuta Nakazawa
2. Nanako Nishiyama
3. Hitoshi Koizumi
4. Kazumasa Kanemaru
5. Chigusa Nakahashi-Oda
6. Akira Shibuya

Reviewed by eLife

The origins and consequences of UPF1 variants in pancreatic adenosquamous carcinoma

1. Jacob T. Polaski
2. Dylan B. Udy
3. Luisa F. Escobar-Hoyos
4. Gokce Askan
5. Steven D. Leach
6. Andrea Ventura
7. Ram Kannan
8. Robert K. Bradley

Reviewed by eLife

A statistical framework for assessing pharmacological response and biomarkers using uncertainty estimates

1. Dennis Wang
2. James Hensman
3. Ginte Kutkaite
4. Tzen S. Toh
5. GDSC Screening Team
6. Jonathan R Dry
7. Julio Saez-Rodriguez
8. Mathew J. Garnett
9. Michael P. Menden
10. Frank Dondelinger

Reviewed by eLife

Phenotypic plasticity underlies local invasion and distant metastasis in colon cancer

1. Andrea Sacchetti
2. Miriam Teeuwssen
3. Mathijs Verhagen
4. Rosalie Joosten
5. Tong Xu
6. Roberto Stabile
7. Berdine van der Steen
8. Martin M. Watson
9. Alem Gusinac
10. Won Kyu Kim
11. Inge Ubink
12. Harmen J.G. van de Werken
13. Arianna Fumagalli
14. Madelon Paauwe
15. Jacco van Rheenen
16. Owen Sansom
17. Onno Kranenburg
18. Riccardo Fodde

Reviewed by eLife

Multiomics integrative analysis reveals antagonistic roles of CBX2 and CBX7 in metabolic reprogramming of breast cancer

1. Mohammad Askandar Iqbal
2. Shumaila Siddiqui
3. Asad Ur Rehman
4. Farid Ahmad Siddiqui
5. Prithvi Singh
6. Bhupender Kumar
7. Daman Saluja

Reviewed by eLife

HIP1 mediates oncogenic transformation and cancer progression through STAT3 signalling

1. Roheet Bantval Rao
2. Antonio Ramos-Montoya
3. Helen Scott
4. Lorraine Berry
5. Stefanie Reichelt
6. Stewart MacArthur
7. Roslin Russell
8. David E Neal
9. Emma Evergren
10. Ian G Mills

Reviewed by eLife

Ral GTPases promote metastasis by controlling biogenesis and organ colonization of exosomes

1. S Ghoroghi
2. B Mary
3. A Larnicol
4. A Klein
5. N Osmani
6. I Busnelli
7. F Delalande
8. N Paul
9. S Halary
10. F Gros
11. L Fouillen
12. AM Haeberle
13. C Royer
14. C Spiegelhalter
15. G André-Grégoire
16. K Murphy
17. P Timpson
18. R Carapito
19. M Blot-Chabaud
20. J Gavard
21. C Carapito
22. N Vitale
23. O Lefebvre
24. JG Goetz
25. V Hyenne

Reviewed by eLife