Biophysics

Ligand sensing enhances bacterial flagellar motor output via stator recruitment

1. Farha Naaz
2. Megha Agrawal
3. Soumyadeep Chakraborty
4. Mahesh S Tirumkudulu
5. KV Venkatesh

• Curated by eLife

  Summary: This is an interesting study reporting an increase in the rotation speed of the E. coli flagellar motor upon the sensing of a non-metabolizable glucose analog (2Dg) by the cell. The authors conclude that this increase is due to an increase in the number of torque-generating stator complexes that drive the motor. Knockout of the trg gene abolished this effect, suggesting that sensing of 2Dg by the Trg chemosensor is responsible. Involvement of membrane potential, the PTS pathway, and the chemotaxis response regulator CheY is ruled out. The manuscript is well-written, and the data are convincing. But the mechanism remains unclear.

  Reviewer #3 opted to reveal their name to the authors in the decision letter after review.

Reviewed by eLife

How subtle changes in 3D structure can create large changes in transcription

1. Jordan Yupeng Xiao
2. Antonina Hafner
3. Alistair N Boettiger

• Curated by eLife

  Summary: The work describes a simple theoretical model for enhancer action that explains several major controversies in the field of long-range gene regulation and the role of topologically associating domains and insulating boundaries in modulating enhancer-promoter interactions. Further, the model makes predictions that can be experimentally tested. This is valuable for the field of gene regulation.

  Reviewer #2 and Reviewer #3 opted to reveal their name to the authors in the decision letter after review.

Reviewed by eLife

The genotype‐phenotype landscape of an allosteric protein

1. Drew S Tack
2. Peter D Tonner
3. Abe Pressman
4. Nathan D Olson
5. Sasha F Levy
6. Eugenia F Romantseva
7. Nina Alperovich
8. Olga Vasilyeva
9. David Ross

Reviewed by Review Commons

Structural characterization of the ANTAR antiterminator domain bound to RNA

1. James L Walshe
2. Rezwan Siddiquee
3. Karishma Patel
4. Sandro F Ataide

• Curated by eLife

  Summary: The reviewers were excited by the structural data, and felt that the structure represents an important advance in our understanding of ANTAR domain proteins. Nonetheless, while the reviewers found the proposed model of ANTAR regulation to be interesting, they raised concerns about the limited evidence in support of this model. In addition to the suggestions in the individual reviews, the authors thought the model could be tested using mutagenesis together with an in vivo or in vitro reporter system, and/or by structural studies of nascent transcripts in transcription complexes with EutV.

Reviewed by eLife

SARS-CoV-2 nucleocapsid protein forms condensates with viral genomic RNA

1. Amanda Jack
2. Luke S. Ferro
3. Michael J. Trnka
4. Eddie Wehri
5. Amrut Nadgir
6. Xammy Huu Wrynla
7. Douglas Fox
8. Katelyn Costa
9. Sarah Stanley
10. Julia Schaletzky
11. Ahmet Yildiz

Reviewed by eLife, ScreenIT

The metal cofactor zinc and interacting membranes modulate SOD1 conformation-aggregation landscape in an in vitro ALS model

1. Achinta Sannigrahi
2. Sourav Chowdhury
3. Bidisha Das
4. Amrita Banerjee
5. Animesh Halder
6. Amaresh Kumar
7. Mohammed Saleem
8. Athi N Naganathan
9. Sanat Karmakar
10. Krishnananda Chattopadhyay

Reviewed by eLife

Characterization of the dynamic resting state of a pentameric ligand-gated ion channel by cryo-electron microscopy and simulations

1. U Rovšnik
2. Y Zhuang
3. L Axelsson
4. BO Forsberg
5. V Lim
6. M Carroni
7. C Blau
8. RJ Howard
9. E Lindahl

Reviewed by eLife

Calponin-homology domain mediated bending of membrane-associated actin filaments

1. Saravanan Palani
2. Sayantika Ghosh
3. Esther Ivorra-Molla
4. Scott Clarke
5. Andrejus Suchenko
6. Mohan K Balasubramanian
7. Darius Vasco Köster

Reviewed by eLife

Through-grid wicking enables high-speed cryoEM specimen preparation

1. Yong Zi Tan
2. John L. Rubinstein

Reviewed by eLife

Proofreading through spatial gradients

1. Vahe Galstyan
2. Kabir Husain
3. Fangzhou Xiao
4. Arvind Murugan
5. Rob Phillips

Reviewed by eLife