Biophysics

Local topology, bifurcations and mutation hot-spots in proteins with SARS-CoV-2 spike protein as an example

1. Xubiao Peng
2. Antti J Niemi

Reviewed by ScreenIT

Molecular mechanism of inhibiting the SARS-CoV-2 cell entry facilitator TMPRSS2 with camostat and nafamostat

1. Tim Hempel
2. Lluís Raich
3. Simon Olsson
4. Nurit P. Azouz
5. Andrea M. Klingler
6. Markus Hoffmann
7. Stefan Pöhlmann
8. Marc E. Rothenberg
9. Frank Noé

Reviewed by ScreenIT

SARS-CoV-2 NSP1 C-terminal (residues 131–180) is an intrinsically disordered region in isolation

1. Amit Kumar
2. Ankur Kumar
3. Prateek Kumar
4. Neha Garg
5. Rajanish Giri

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Role of spatial patterning of N-protein interactions in SARS-CoV-2 genome packaging

1. Ian Seim
2. Christine A. Roden
3. Amy S. Gladfelter

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Structure of SARS-CoV-2 ORF8, a rapidly evolving immune evasion protein

1. Thomas G. Flower
2. Cosmo Z. Buffalo
3. Richard M. Hooy
4. Marc Allaire
5. Xuefeng Ren
6. James H. Hurley

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Inhibition of SARS-CoV-2 polymerase by nucleotide analogs: a single molecule perspective

1. Mona Seifert
2. Subhas Chandra Bera
3. Pauline van Nies
4. Robert N. Kirchdoerfer
5. Ashleigh Shannon
6. Thi-Tuyet-Nhung Le
7. Xiangzhi Meng
8. Hongjie Xia
9. James M. Wood
10. Lawrence D. Harris
11. Flávia S. Papini
12. Jamie J. Arnold
13. Steven C. Almo
14. Tyler L. Grove
15. Pei-Yong Shi
16. Yan Xiang
17. Bruno Canard
18. Martin Depken
19. Craig E. Cameron
20. David Dulin

• Curated by eLife

  Evaluation Summary:

  This work presented in this manuscript paper may advance our understanding of an important class of anti-viral drugs (nucleoside analogs) that target polymerase enzymes by being directly incorporated into the product strand. This class of drugs is known to be quite diverse in their precise mechanisms of action, yet many of the particular details have remained elusive, often due to experimental limitations. The current study employs a single-molecule magnetic-tweezers platform to provide a new paradigm for the mechanism of drug remdesivir against the SARS-CoV-2 polymerase target. The authors propose that remdesivir does not prevent the complete viral RNA synthesis but causes an increase of polymerase pausing and back tracking. This paper is of broad interest to readers and scientists working on SARS-CoV-2 and general nucleotide inhibitors. The work includes the single molecule characterization of other NAs.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife, ScreenIT

A Blueprint for High Affinity SARS-CoV-2 Mpro Inhibitors from Activity-Based Compound Library Screening Guided by Analysis of Protein Dynamics

1. Jonas Gossen
2. Simone Albani
3. Anton Hanke
4. Benjamin P. Joseph
5. Cathrine Bergh
6. Maria Kuzikov
7. Elisa Costanzi
8. Candida Manelfi
9. Paola Storici
10. Philip Gribbon
11. Andrea R. Beccari
12. Carmine Talarico
13. Francesca Spyrakis
14. Erik Lindahl
15. Andrea Zaliani
16. Paolo Carloni
17. Rebecca C. Wade
18. Francesco Musiani
19. Daria B. Kokh
20. Giulia Rossetti

Reviewed by ScreenIT

pH Effect on the Dynamics of SARS-CoV-2 Main Protease (M ^pro )

1. Shilpa Sharma
2. Shashank Deep

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On the molecular mechanism of SARS-CoV-2 retention in the upper respiratory tract

1. Kristina A. Paris
2. Ulises Santiago
3. Carlos J. Camacho

Reviewed by ScreenIT

Structure and inhibition of the SARS-CoV-2 main protease reveal strategy for developing dual inhibitors against M ^pro and cathepsin L

1. Michael Dominic Sacco
2. Chunlong Ma
3. Panagiotis Lagarias
4. Ang Gao
5. Julia Alma Townsend
6. Xiangzhi Meng
7. Peter Dube
8. Xiujun Zhang
9. Yanmei Hu
10. Naoya Kitamura
11. Brett Hurst
12. Bart Tarbet
13. Michael Thomas Marty
14. Antonios Kolocouris
15. Yan Xiang
16. Yu Chen
17. Jun Wang

Reviewed by ScreenIT