Biophysics

Structural Insights of SARS-CoV-2 Spike Protein from Delta and Omicron Variants

1. Ali Sadek
2. David Zaha
3. Mahmoud Salama Ahmed

Reviewed by ScreenIT

Post-Translational Modifications Optimize the Ability of SARS-CoV-2 Spike for Effective Interaction with Host Cell Receptors

1. Karan Kapoor
2. Tianle Chen
3. Emad Tajkhorshid

Reviewed by ScreenIT

Predicting potential SARS-CoV-2 mutations of concern via full quantum mechanical modelling

1. Marco Zaccaria
2. Luigi Genovese
3. Brigitte E. Lawhorn
4. William Dawson
5. Andrew S. Joyal
6. Jingqing Hu
7. Patrick Autissier
8. Takahito Nakajima
9. Welkin E. Johnson
10. Ismael Fofana
11. Michael Farzan
12. Babak Momeni

Reviewed by ScreenIT

Thermal analysis of protein stability and ligand binding in complex media

1. Matthew W. Eskew
2. Albert S. Benight

Reviewed by ScreenIT

SARS-CoV-2 variants impact RBD conformational dynamics and ACE2 accessibility

1. Mariana Valério
2. Luís Borges-Araújo
3. Manuel N. Melo
4. Diana Lousa
5. Cláudio M. Soares

Reviewed by ScreenIT

In silico study on the effects of disulfide bonds in ORF8 of SARS-CoV-2

1. Yadi Cheng
2. Xubiao Peng

Reviewed by ScreenIT

Molecular pathology of the R117H cystic fibrosis mutation is explained by loss of a hydrogen bond

1. Márton A Simon
2. László Csanády

• Curated by eLife

  Evaluation Summary:

  Multiple inherited mutations in the epithelial CFTR anion-permeable channel cause cystic fibrosis through different molecular mechanisms that can be targeted by different types of drugs to treat the disease. Drawing from available structural information and double-mutant cycle analysis of patch-clamp recordings, Simon and Csanády find that one of the most common CFTR disease-causing mutations, R117H, disrupts an interaction between the R117 side-chain and a main-chain carbonyl that selectively stabilizes the open state of the channel. These findings may open new paths of exploration for treating patients carrying this mutation, and provide important mechanistic constraints towards understanding the gating mechanism of CFTR proteins.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

Probing the mutational landscape of the SARS-CoV-2 spike protein via quantum mechanical modeling of crystallographic structures

1. Marco Zaccaria
2. Luigi Genovese
3. William Dawson
4. Viviana Cristiglio
5. Takahito Nakajima
6. Welkin Johnson
7. Michael Farzan
8. Babak Momeni

Reviewed by ScreenIT

Label-free imaging of M1 and M2 macrophage phenotypes in the human dermis in vivo using two-photon excited FLIM

1. Marius Kröger
2. Jörg Scheffel
3. Evgeny A Shirshin
4. Johannes Schleusener
5. Martina C Meinke
6. Jürgen Lademann
7. Marcus Maurer
8. Maxim E Darvin

• Curated by eLife

  Evaluation Summary:

  Kröger et al use 2-photon FLIM tomography to perform correlative imaging on in vitro, ex vivo and in vivo blood and skin cells to determine characteristic NADPH fluorescence lifetimes for M1 and M2 ends of macrophage spectrum. Interestingly, M1 and M2 macrophages, and all other tissue cells, had distinctive lifetime features leading to robust prediction of phenotypes, with ground trust defined by cytokine staining. They generate a decision tree that has ~90% accuracy in identifying M1 and M2 based on FLIM parameters and additional information. The ability to use two photon fluorescence lifetime tomography of NADPH fluorescence to identify macrophages and their inflammatory status in human tissues should open opportunities in experimental medicine and eventually medical diagnosis.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

The versatile regulation of K2P channels by polyanionic lipids of the phosphoinositide and fatty acid metabolism

1. Elena B. Riel
2. Björn C. Jürs
3. Sönke Cordeiro
4. Marianne Musinszki
5. Marcus Schewe
6. Thomas Baukrowitz

Reviewed by Biophysics Colab