Bioengineering

  1. Free-Form Microfluidic Microneedle Array Patches

    1. Ian A. Coates
    2. Madison M. Driskill
    3. Netra U. Rajesh
    4. Gabriel Lipkowitz
    5. Dan Ilyin
    6. Yue Xu
    7. Maria T. Dulay
    8. Gunilla B. Jacobson
    9. Jillian L. Perry
    10. Shaomin Tian
    11. Joseph M. DeSimone

    Reviewed by Arcadia Science

    1 evaluationAppears in 1 listLatest version Latest activity

  2. Broad-spectrum immune suppression encoded in self-amplifying RNA enables non-cytotoxic, non-immunostimulatory, externally controllable transgene expression

    1. Tony KY Lim
    2. Anne Ritoux
    3. Luke W Paine
    4. Larissa Ferguson
    5. Tawab Abdul
    6. Ewan St John Smith

    • Curated by eLife

      eLife Assessment

      In this manuscript, Lim and collaborators present a useful system for developing self-amplifying RNA that should not provoke a strong host inflammatory response. However, some of the claims are incomplete; additional experiments to investigate the effects on translation of the gene of interest and replication efficiency of the self-amplifying RNA could strengthen the manuscript.

    Reviewed by eLife

    4 evaluationsAppears in 1 listLatest version Latest activity

  3. Isolating Small Extracellular Vesicles from Small Volumes of Blood Plasma using size exclusion chromatography and density gradient ultracentrifugation: A Comparative Study

    1. Fang Kong
    2. Megha Upadya
    3. Andrew See Wong Weng
    4. Rinkoo Dalan
    5. Ming Dao

    • Curated by eLife

      eLife Assessment

      This work provides a simple, rapid and valuable protocol for the isolation of small extracellular vesicles from small volumes of plasma, using two well-known methodologies, in tandem: size exclusion chromatography (SEC) and density gradient ultracentrifugation (DGUC). The authors exhaustively test these methodologies separately and in combination, showing superior results for the SEC-DGUC in terms of purity and yield. The results obtained in this work are convincing, using multiple state-of-art methodologies for the characterization of the isolates that support their conclusions.

    Reviewed by eLife

    6 evaluationsAppears in 1 listLatest version Latest activity

  4. Development of antigen-dextramers for detection and evaluation of CAR T cells

    1. Rasmus U. W. Friis
    2. Maria Ormhøj
    3. Cecilie S. Krüger-Jensen
    4. Markus Barden
    5. Keerthana Ramanathan
    6. Mikkel R. Hansen
    7. Hinrich Abken
    8. Sine R. Hadrup

    Reviewed by Arcadia Science

    1 evaluationAppears in 1 listLatest version Latest activity

  5. Lab-in-the-loop therapeutic antibody design with deep learning

    1. Nathan C. Frey
    2. Isidro Hötzel
    3. Samuel D. Stanton
    4. Ryan Kelly
    5. Robert G. Alberstein
    6. Emily Makowski
    7. Karolis Martinkus
    8. Daniel Berenberg
    9. Jack Bevers
    10. Tyler Bryson
    11. Pamela Chan
    12. Alicja Czubaty
    13. Tamica D’Souza
    14. Henri Dwyer
    15. Anna Dziewulska
    16. James W. Fairman
    17. Allen Goodman
    18. Jennifer Hofmann
    19. Henry Isaacson
    20. Aya Ismail
    21. Samantha James
    22. Taylor Joren
    23. Simon Kelow
    24. James R. Kiefer
    25. Matthieu Kirchmeyer
    26. Joseph Kleinhenz
    27. James T. Koerber
    28. Julien Lafrance-Vanasse
    29. Andrew Leaver-Fay
    30. Jae Hyeon Lee
    31. Edith Lee
    32. Donald Lee
    33. Wei-Ching Liang
    34. Joshua Yao-Yu Lin
    35. Sidney Lisanza
    36. Andreas Loukas
    37. Jan Ludwiczak
    38. Sai Pooja Mahajan
    39. Omar Mahmood
    40. Homa Mohammadi-Peyhani
    41. Santrupti Nerli
    42. Ji Won Park
    43. Jaewoo Park
    44. Stephen Ra
    45. Sarah Robinson
    46. Saeed Saremi
    47. Franziska Seeger
    48. Imee Sinha
    49. Anna M. Sokol
    50. Natasa Tagasovska
    51. Hao To
    52. Edward Wagstaff
    53. Amy Wang
    54. Andrew M. Watkins
    55. Blair Wilson
    56. Shuang Wu
    57. Karina Zadorozhny
    58. John Marioni
    59. Aviv Regev
    60. Yan Wu
    61. Kyunghyun Cho
    62. Richard Bonneau
    63. Vladimir Gligorijević

    Reviewed by Arcadia Science

    4 evaluationsAppears in 1 listLatest version Latest activity

  6. CRISPR-Edited DPSCs, Constitutively Expressing BDNF Enhance Dentin Regeneration in Injured Teeth

    1. Ji Hyun Kim
    2. Muhammad Irfan
    3. Sreelekshmi Sreekumar
    4. Stephanie Kim
    5. Atsawasuwan Phimon
    6. Seung Chung

    • Curated by eLife

      eLife Assessment

      This study provides a potentially useful investigation into the positive role of BDNF/TrkB signaling in implanted dental pulp stem cells to enhance dentin regeneration in the context of dental caries. Some of the key methods used need to be much better documented, and the data should be strengthened and added to in support of several of the claims of functional benefit, which are inadequately supported at present. Additional details for the validation of the reagents and techniques are needed to support the interpretation of the results.

    Reviewed by eLife

    4 evaluationsAppears in 1 listLatest version Latest activity

  7. Mitochondrial adenine base editing of mouse somatic tissues via adeno-associated viral delivery

    1. Christian D Mutti
    2. Lindsey Van Haute
    3. Lucia Luengo-Gutierrez
    4. Keira Turner
    5. Pedro Silva-Pinheiro
    6. Michal Minczuk

    • Curated by eLife

      eLife Assessment

      The authors have demonstrated the use of adenine base editors delivered via adeno-associated viruses to introduce edits in the mitochondrial genome. The manuscript describes the methodology well, and the conclusions are convincingly supported by the results. The valuable results highlight the potential of these base editors to model mtDNA variations in somatic tissues in animal models.

    Reviewed by eLife

    3 evaluationsAppears in 1 listLatest version Latest activity

  8. Scaly-Tail Organ Enhances Static Stability during Pel’s Scaly-tailed Flying Squirrels’ Arboreal Locomotion

    1. Andrew K. Schulz
    2. Mrudul Chellapurath
    3. Pranav C. Khandelwal
    4. SeyedReza Rezaei
    5. Stefan Merker
    6. Ardian Jusufi

    Reviewed by preLights

    1 evaluationAppears in 1 listLatest version Latest activity

  9. Semantical and Geometrical Protein Encoding Toward Enhanced Bioactivity and Thermostability

    1. Yang Tan
    2. Bingxin Zhou
    3. Lirong Zheng
    4. Guisheng Fan
    5. Liang Hong

    • Curated by eLife

      eLife Assessment

      ProtSSN is a valuable approach that generates protein embeddings by integrating sequence and structural information, demonstrating improved prediction of mutation effects on thermostability compared to competing models. The evidence supporting the authors' claims is compelling, with well-executed comparisons. This work will be of particular interest to researchers in bioinformatics and structural biology, especially those focused on protein function and stability.

    Reviewed by eLife

    11 evaluationsAppears in 1 listLatest version Latest activity

  10. DyAb: sequence-based antibody design and property prediction in a low-data regime

    1. Joshua Yao-Yu Lin
    2. Jennifer L. Hofmann
    3. Andrew Leaver-Fay
    4. Wei-Ching Liang
    5. Stefania Vasilaki
    6. Edith Lee
    7. Pedro O. Pinheiro
    8. Natasa Tagasovska
    9. James R. Kiefer
    10. Yan Wu
    11. Franziska Seeger
    12. Richard Bonneau
    13. Vladimir Gligorijevic
    14. Andrew Watkins
    15. Kyunghyun Cho
    16. Nathan C. Frey

    Reviewed by Arcadia Science

    5 evaluationsAppears in 1 listLatest version Latest activity
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