1. Evaluation of Machine Learning-Assisted Directed Evolution Across Diverse Combinatorial Landscapes

    This article has 6 authors:
    1. Francesca-Zhoufan Li
    2. Jason Yang
    3. Kadina E. Johnston
    4. Emre Gürsoy
    5. Yisong Yue
    6. Frances H. Arnold

    Reviewed by Arcadia Science

    This article has 10 evaluationsAppears in 1 listLatest version Latest activity
  2. Applied tutorial for the design and fabrication of biomicrofluidic devices by resin 3D printing

    This article has 3 authors:
    1. Hannah.B. Musgrove
    2. Megan.A. Catterton
    3. Rebecca.R. Pompano

    Reviewed by Arcadia Science

    This article has 1 evaluationAppears in 3 listsLatest version Latest activity
  3. The flexible stalk domain of sTREM2 modulates its interactions with phospholipids in the brain

    This article has 6 authors:
    1. David Saeb
    2. Emma E Lietzke
    3. Daisy I Fuchs
    4. Emma C Aldrich
    5. Kimberley D Bruce
    6. Kayla G Sprenger
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This useful manuscript addresses some key molecular mechanisms on the neuroprotective roles of soluble TREM2 in neurodegenerative diseases. Thw study will advance our understanding of TREM2 mutations, particularly on the damaging effect of known TREM2 mutations, and also explain why soluble TREM2 can antagonize Aβ aggregation. However, the primary experimental method, MD simulations, suffers from limited sampling, rendering the results incomplete for definite conclusions.

    Reviewed by eLife

    This article has 4 evaluationsAppears in 1 listLatest version Latest activity
  4. Semantical and Geometrical Protein Encoding Toward Enhanced Bioactivity and Thermostability

    This article has 5 authors:
    1. Yang Tan
    2. Bingxin Zhou
    3. Lirong Zheng
    4. Guisheng Fan
    5. Liang Hong
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      ProtSSN is a valuable approach that generates protein embeddings by integrating sequence and structural information, demonstrating improved prediction of mutation effects on thermostability compared to competing models. The evidence supporting the authors' claims is solid, with well-executed comparisons. This work will be of particular interest to researchers in bioinformatics and structural biology, especially those focused on protein function and stability.

    Reviewed by eLife

    This article has 7 evaluationsAppears in 1 listLatest version Latest activity
  5. Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation

    This article has 22 authors:
    1. Mami Matsuo-Takasaki
    2. Sho Kambayashi
    3. Yasuko Hemmi
    4. Tamami Wakabayashi
    5. Tomoya Shimizu
    6. Yuri An
    7. Hidenori Ito
    8. Kazuhiro Takeuchi
    9. Masato Ibuki
    10. Terasu Kawashima
    11. Rio Masayasu
    12. Manami Suzuki
    13. Naoki Nishishita
    14. Yoshikazu Kawai
    15. Masafumi Umekage
    16. Tomoaki M Kato
    17. Michiya Noguchi
    18. Koji Nakade
    19. Yukio Nakamura
    20. Tomoyuki Nakaishi
    21. Masayoshi Tsukahara
    22. Yohei Hayashi
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This comprehensive and compelling study presents a robust, cost-effective method for expanding pluripotent stem cells. The authors have identified a media condition that maintains iPSCs in suspension cultures by inhibiting the PKCβ and Wnt signaling pathways. The manuscript is important for the pluripotent stem cell field as it seeks robust and economical approaches to expand iPSCs at scale for high throughput screens and preclinical studies. While the authors have tested their media and protocol on a few lines, given the variability of iPSCs, further testing across more cell lines and in different laboratory settings will be crucial to evaluate its reproducibility.

    Reviewed by eLife

    This article has 9 evaluationsAppears in 1 listLatest version Latest activity
  6. HiExM: high-throughput expansion microscopy enables scalable super-resolution imaging

    This article has 8 authors:
    1. John H Day
    2. Catherine Marin Della Santina
    3. Pema Maretich
    4. Alexander L Auld
    5. Kirsten K Schnieder
    6. Tay Shin
    7. Edward S Boyden
    8. Laurie A Boyer
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This important study develops a high throughput version of expansion microscopy that can be performed in 96-well plates. The engineered technology is convincing and compatible with standard microplates and automated microscopes and thus will be of broad interest. The application to hiPCS-derived cardiomyocytes treated with doxorubicin provides a solid proof-of-concept demonstrating the potential for high-throughput analysis.

    Reviewed by eLife

    This article has 15 evaluationsAppears in 1 listLatest version Latest activity
  7. Patch-walking: Coordinated multi-pipette patch clamp for efficiently finding synaptic connections

    This article has 11 authors:
    1. Mighten C Yip
    2. Mercedes M Gonzalez
    3. Colby F Lewallen
    4. Corey R Landry
    5. Ilya Kolb
    6. Bo Yang
    7. William M Stoy
    8. Ming-fai Fong
    9. Matthew JM Rowan
    10. Edward S Boyden
    11. Craig R Forest
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      This technical study presents a novel sampling strategy for detecting synaptic coupling between neurons from dual pipette patch-clamp recordings in acute slices of mammalian brain tissue in vitro. The authors present solid evidence that this strategy, which incorporates automated patch clamp electrode positioning and cleaning for reuse with strategic neuron targeting, has the potential to substantially improve the efficiency of neuronal sampling with paired recordings. This technique and the extensions discussed will be useful for neuroscientists wanting to apply or already conducting automated multi-pipette patch clamp recording electrophysiology experiments in vitro for neuron connectivity analyses.

    Reviewed by eLife

    This article has 9 evaluationsAppears in 1 listLatest version Latest activity
  8. An automated microfluidic platform for toxicity testing based on Caenorhabditis elegans

    This article has 9 authors:
    1. Jiaying Wu
    2. Jing Xi
    3. Zhouhai Zhu
    4. Qiyuan Peng
    5. Chunbo Liu
    6. Ziqian Wan
    7. Yiyi Cao
    8. Xiang Chen
    9. Yang Luan

    Reviewed by Arcadia Science

    This article has 10 evaluationsAppears in 1 listLatest version Latest activity
  9. Development of a new genotype–phenotype linked antibody screening system

    This article has 9 authors:
    1. Takashi Watanabe
    2. Hikaru Hata
    3. Yoshiki Mochizuki
    4. Fumie Yokoyama
    5. Tomoko Hasegawa
    6. Naveen Kumar
    7. Tomohiro Kurosaki
    8. Osamu Ohara
    9. Hidehiro Fukuyama
    This article has been curated by 1 group:
    • Curated by eLife

      eLife Assessment

      The useful studies described here are broadly applicable to all antibody discovery subfields, even though they are not a significant improvement over published methods. The findings are incomplete with respect to the methodology, since details that are crucial in order to repeat the experiments are lacking (such as a timestamp). They also do not take into account multiple recent papers that have tested similar strategies. These studies will be of interest to a specialized audience working on generating antibodies to infectious agents.

    Reviewed by eLife

    This article has 7 evaluationsAppears in 1 listLatest version Latest activity
  10. Engineered Nanotopographies Induce Transient Openings in the Nuclear Membrane

    This article has 17 authors:
    1. Einollah Sarikhani
    2. Vrund Patel
    3. Zhi Li
    4. Dhivya Pushpa Meganathan
    5. Keivan Rahmani
    6. Leah Sadr
    7. Ryan Hosseini
    8. Diether Visda
    9. Shivani Shukla
    10. Hamed Naghsh-Nilchi
    11. Adarsh Balaji
    12. Gillian McMahon
    13. Shaoming Chen
    14. Johannes Schöneberg
    15. Colleen A. McHugh
    16. Lingyan Shi
    17. Zeinab Jahed

    Reviewed by preLights

    This article has 1 evaluationAppears in 1 listLatest version Latest activity
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