Biochemistry

TMPRSS2 Inhibitor Discovery Facilitated through an In Silico and Biochemical Screening Platform

1. Amanda L. Peiffer
2. Julie M. Garlick
3. Yujin Wu
4. Jesse W. Wotring
5. Sahil Arora
6. Alexander S. Harmata
7. Daniel A. Bochar
8. Corey J. Stephenson
9. Matthew B. Soellner
10. Jonathan Z. Sexton
11. Charles L. Brooks
12. Anna K. Mapp

Reviewed by ScreenIT

Targeted in situ cross-linking mass spectrometry and integrative modeling reveal the architectures of three proteins from SARS-CoV-2

1. Moriya Slavin
2. Joanna Zamel
3. Keren Zohar
4. Tsiona Eliyahu
5. Merav Braitbard
6. Esther Brielle
7. Leah Baraz
8. Miri Stolovich-Rain
9. Ahuva Friedman
10. Dana G. Wolf
11. Alexander Rouvinski
12. Michal Linial
13. Dina Schneidman-Duhovny
14. Nir Kalisman

Reviewed by ScreenIT

Free fatty acid binding pocket in the locked structure of SARS-CoV-2 spike protein

1. Christine Toelzer
2. Kapil Gupta
3. Sathish K. N. Yadav
4. Ufuk Borucu
5. Andrew D. Davidson
6. Maia Kavanagh Williamson
7. Deborah K. Shoemark
8. Frederic Garzoni
9. Oskar Staufer
10. Rachel Milligan
11. Julien Capin
12. Adrian J. Mulholland
13. Joachim Spatz
14. Daniel Fitzgerald
15. Imre Berger
16. Christiane Schaffitzel

Reviewed by ScreenIT

A metal ion orients SARS-CoV-2 mRNA to ensure accurate 2′-O methylation of its first nucleotide

1. Thiruselvam Viswanathan
2. Anurag Misra
3. Siu-Hong Chan
4. Shan Qi
5. Nan Dai
6. Shailee Arya
7. Luis Martinez-Sobrido
8. Yogesh K. Gupta

Reviewed by ScreenIT

Relative Mutant N501Y SARS-CoV-2 Spike Protein RBD Inhibition of Anti-Spike Protein IgG and ACE-2 Binding to Spike Protein Species

1. Melvin E. Klegerman
2. Jeffrey D. Cirillo
3. David D. McPherson

Reviewed by ScreenIT

Identifying SARS-CoV-2 antiviral compounds by screening for small molecule inhibitors of Nsp3 papain-like protease

1. Chew Theng Lim
2. Kang Wei Tan
3. Mary Wu
4. Rachel Ulferts
5. Lee A. Armstrong
6. Eiko Ozono
7. Lucy S. Drury
8. Jennifer C. Milligan
9. Theresa U. Zeisner
10. Jingkun Zeng
11. Florian Weissmann
12. Berta Canal
13. Ganka Bineva-Todd
14. Michael Howell
15. Nicola O'Reilly
16. Rupert Beale
17. Yogesh Kulathu
18. Karim Labib
19. John F.X. Diffley

Reviewed by ScreenIT

Highly synergistic combinations of nanobodies that target SARS-CoV-2 and are resistant to escape

1. Fred D Mast
2. Peter C Fridy
3. Natalia E Ketaren
4. Junjie Wang
5. Erica Y Jacobs
6. Jean Paul Olivier
7. Tanmoy Sanyal
8. Kelly R Molloy
9. Fabian Schmidt
10. Magdalena Rutkowska
11. Yiska Weisblum
12. Lucille M Rich
13. Elizabeth R Vanderwall
14. Nicholas Dambrauskas
15. Vladimir Vigdorovich
16. Sarah Keegan
17. Jacob B Jiler
18. Milana E Stein
19. Paul Dominic B Olinares
20. Louis Herlands
21. Theodora Hatziioannou
22. D Noah Sather
23. Jason S Debley
24. David Fenyö
25. Andrej Sali
26. Paul D Bieniasz
27. John D Aitchison
28. Brian T Chait
29. Michael P Rout

• Curated by eLife

  Evaluation Summary:

  The manuscript by Mast et al. describes an impressive collection of new nanobodies against SARS-CoV-2 spike, which probably provides the most complete coverage of the accessible epitopes of spike to date. The authors thoroughly characterize biophysical and functional properties of the nanobodies and set an example of how to best combine multiple nanobodies to target a pathogen. As the latest in a long series of SARS-CoV-2 nanobody papers, this study stands out for its completeness, although it does not provide a novel mechanism of action or biological insights.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife, ScreenIT

A High-Throughput Radioactivity-Based Assay for Screening SARS-CoV-2 nsp10-nsp16 Complex

1. Aliakbar Khalili Yazdi
2. Fengling Li
3. Kanchan Devkota
4. Sumera Perveen
5. Pegah Ghiabi
6. Taraneh Hajian
7. Albina Bolotokova
8. Masoud Vedadi

Reviewed by ScreenIT

Functional diversity among cardiolipin binding sites on the mitochondrial ADP/ATP carrier

1. Nanami Senoo
2. Dinesh K Chinthapalli
3. Matthew G Baile
4. Vinaya K Golla
5. Bodhisattwa Saha
6. Abraham O Oluwole
7. Oluwaseun B Ogunbona
8. James A Saba
9. Teona Munteanu
10. Yllka Valdez
11. Kevin Whited
12. Macie S Sheridan
13. Dror Chorev
14. Nathan N Alder
15. Eric R May
16. Carol V Robinson
17. Steven M Claypool

Reviewed by preLights

A high-throughput fluorescence polarization assay to discover inhibitors of arenavirus and coronavirus exoribonucleases

1. Sergio Hernández
2. Mikael Feracci
3. Carolina Trajano De Jesus
4. Priscila El-Kazzi
5. Rafik Kaci
6. Laura Garlatti
7. Etienne Decroly
8. Bruno Canard
9. François Ferron
10. Karine Alvarez

Reviewed by ScreenIT