Biochemistry

Altered fibrin clot structure and dysregulated fibrinolysis contribute to thrombosis risk in severe COVID-19

1. Malgorzata Wygrecka
2. Anna Birnhuber
3. Benjamin Seeliger
4. Laura Michalick
5. Oleg Pak
6. Astrid-Solveig Schultz
7. Fabian Schramm
8. Martin Zacharias
9. Gregor Gorkiewicz
10. Sascha David
11. Tobias Welte
12. Julius J. Schmidt
13. Norbert Weissmann
14. Ralph T. Schermuly
15. Guillermo Barreto
16. Liliana Schaefer
17. Philipp Markart
18. Markus C. Brack
19. Stefan Hippenstiel
20. Florian Kurth
21. Leif E. Sander
22. Martin Witzenrath
23. Wolfgang M. Kuebler
24. Grazyna Kwapiszewska
25. Klaus T. Preissner

Reviewed by ScreenIT

Copper(II) Gluconate Boosts the Anti-SARS-CoV-2 Effect of Disulfiram In Vitro

1. Luyan Xu
2. Wei Xu
3. Simeng Zhao
4. Suwen Zhao
5. Lu Lu
6. Bo-Lin Lin

Reviewed by ScreenIT

The Nuts and Bolts of SARS-CoV-2 Spike Receptor-Binding Domain Heterologous Expression

1. Mariano Maffei
2. Linda Celeste Montemiglio
3. Grazia Vitagliano
4. Luigi Fedele
5. Shaila Sellathurai
6. Federica Bucci
7. Mirco Compagnone
8. Valerio Chiarini
9. Cécile Exertier
10. Alessia Muzi
11. Giuseppe Roscilli
12. Beatrice Vallone
13. Emanuele Marra

Reviewed by ScreenIT

Competitive binding of MatP and topoisomerase IV to the MukB dimerization hinge

1. Gemma L. M. Fisher
2. Jani R. Bolla
3. Karthik V. Rajasekar
4. Jarno Mäkelä
5. Rachel Baker
6. Man Zhou
7. Josh P. Prince
8. Mathew Stracy
9. Carol V. Robinson
10. Lidia K. Arciszewska
11. David J. Sherratt

• Curated by eLife

  Evaluation Summary:

  This paper is of potential interest to an audience of biochemists, cell biologists, and structural biologists working in the area of chromosome organization and segregation. A wide range of in vitro methods is used to provide compelling biochemical evidence for the interaction of MatP and ParEC at the hinge of MukB, the condensin of Enterobacteria. However, the evidence supporting the significance of these interactions in vivo is less strong, limiting the biological implications of the elegant biochemical findings.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. The reviewers remained anonymous to the authors.)

Reviewed by eLife

Prolific Induction of IL-6 in Human Cells by SARS-CoV-2-derived Peptide is Attenuated by Recombinant Human Anti-inflammatory Cytokines made in planta

1. Pieter H. Anborgh
2. Igor Kolotilin
3. Nisha Owens
4. Abdulla Azzam Mahboob

Reviewed by ScreenIT

Proton transfer pathway in anion channelrhodopsin-1

1. Masaki Tsujimura
2. Keiichi Kojima
3. Shiho Kawanishi
4. Yuki Sudo
5. Hiroshi Ishikita

• Curated by eLife

  Evaluation Summary:

  The authors report continuum electrostatics, molecular dynamics and QM/MM simulations to probe the protonation pattern of key residues in anion channelrhodopsin (GtACR1). The findings provide new mechanistic insights into the gating mechanism of GtACR1, and the study will be of potential interest for the community focused on biophysical chemistry, protein simulations and optogenetics. More generally, the study helps highlight that due to potential compensatory effects, care needs to be exercised when interpreting absorption spectra of mutant proteins.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Ultrafast, one-step, and microwave heating-based synthesis of DNA/RNA-AuNP conjugates

1. Mengqi Huang
2. Erhu Xiong
3. Menglu Hu
4. Huahua Yue
5. Tian Tian
6. Debin Zhu
7. Xiaoming Zhou

Reviewed by ScreenIT

pH-dependent polymorphism of the structure of SARS-CoV-2 nsp7

1. Yeongjoon Lee
2. Marco Tonelli
3. Mehdi Rahimi
4. Thomas K. Anderson
5. Robert N. Kirchdoerfer
6. Katherine Henzler-Wildman
7. Woonghee Lee

Reviewed by ScreenIT

Structure of mycobacterial CIII2CIV2 respiratory supercomplex bound to the tuberculosis drug candidate telacebec (Q203)

1. David J Yanofsky
2. Justin M Di Trani
3. Sylwia Król
4. Rana Abdelaziz
5. Stephanie A Bueler
6. Peter Imming
7. Peter Brzezinski
8. John L Rubinstein

• Curated by eLife

  Evaluation Summary:

  The manuscript by Yanofsky et al. describes the high-resolution structure of the CIII/CIV super-complex from Mycobacterium smegmatis bound to the anti-tuberculosis drug Q203 which is currently in clinical trials. The authors also provide biochemical data for inhibition of purified CIII/CIV and add important new information regarding the mechanism of Q203. This is an important contribution that will be of broad interest to the field of bioenergetic and tuberculosis, as telacebec represents one of few novel drug classes with potential for treatment of tuberculosis and other mycobacterial infections.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 agreed to share their name with the authors.)

Reviewed by eLife

Structural basis of polyamine transport by human ATP13A2 (PARK9)

1. Sue Im Sim
2. Sören von Bülow
3. Gerhard Hummer
4. Eunyong Park

Reviewed by Biophysics Colab