Biochemistry

Mutations in the spike RBD of SARS-CoV-2 omicron variant may increase infectivity without dramatically altering the efficacy of current multi-dosage vaccinations

1. Bingrui Li
2. Xin Lu
3. Kathleen M. McAndrews
4. Raghu Kalluri

Reviewed by ScreenIT

Cell-surface tethered promiscuous biotinylators enable comparative small-scale surface proteomic analysis of human extracellular vesicles and cells

1. Lisa L Kirkemo
2. Susanna K Elledge
3. Jiuling Yang
4. James R Byrnes
5. Jeff E Glasgow
6. Robert Blelloch
7. James A Wells

• Curated by eLife

  Evaluation Summary:

  This report describes a new technique to detect the surface proteome of normal and myc-transformed cells in relation to extracellular vesicles from the same cells. The data obtained from this comparison may be useful in evaluating cell surface and extracellular vesicle marker proteins that may be of diagnostic value. The article could possibly be more interesting if the actual proteomic results of control vs Myc and cells vs extracellular vesicles were more extensively exploited.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #1 and Reviewer #2 agreed to share their names with the authors.)

Reviewed by eLife

A proteomic perspective and involvement of cytokines in SARS-CoV-2 infection

1. Sarena Banu
2. Ramakrishnan Nagaraj
3. Mohammed M. Idris

Reviewed by ScreenIT

The High Transmission of SARS-CoV-2 Omicron (B.1.1.529) Variant is Not Only Due to Its hACE2 binding: A Free Energy of Perturbation Study

1. Filip Fratev

Reviewed by ScreenIT

Interactions of SARS-CoV-2 protein E with cell junctions and polarity PDZ-containing proteins

1. Yanlei Zhu
2. Flavio Alvarez
3. Nicolas Wolff
4. Ariel Mechaly
5. Sébastien Brûlé
6. Benoit Neitthoffer
7. Sandrine Etienne-Manneville
8. Ahmed Haouz
9. Batiste Boëda
10. Célia Caillet-Saguy

Reviewed by ScreenIT

Alzheimer's Aβ assembly binds sodium pump and blocks endothelial NOS activity via ROS-PKC pathway in brain vascular endothelial cells

1. Tomoya Sasahara
2. Kaori Satomura
3. Mari Tada
4. Akiyoshi Kakita
5. Minako Hoshi

• Curated by eLife

  Evaluation Summary:

  This paper examines the effects of amylospheroids, highly neurotoxic assemblies of β-amyloid, on aortic function and on cultured cells. The authors propose that the interaction of amylospheroids with the sodium pump in endothelial cells induces production of reactive oxygen species to ultimately comprise nitric oxide generation. The study provides some new insight into mechanisms underlying brain blood vessel dysfunction and will be interesting neuroscientists who study neurovascular contribution to neurodegenerative diseases. The conclusions of the manuscript are supported by the data, but alternative approaches would make the study stronger.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. Reviewer #2 agreed to share their name with the authors.)

Reviewed by eLife

Possible Interference in Protein – Protein interaction as a new approach in microinhibition of respiratory pathogens on nasal– oral epithelium: An early on-screen study with reference toSARS-Cov-2–ACE2 binding interference

1. Debatosh Datta
2. Suyash Pant
3. Devendra Kumar Dhaked
4. Somasundaram Arumugam
5. Ravichandiran Velayutham
6. Pallab Datta

Reviewed by ScreenIT

Delta Variant with P681R Critical Mutation Revealed by Ultra-Large Atomic-Scale Ab Initio Simulation: Implications for the Fundamentals of Biomolecular Interactions

1. Puja Adhikari
2. Bahaa Jawad
3. Praveen Rao
4. Rudolf Podgornik
5. Wai-Yim Ching

Reviewed by ScreenIT

Main protease mutants of SARS-CoV-2 variants remain susceptible to nirmatrelvir

1. Sven Ullrich
2. Kasuni B. Ekanayake
3. Gottfried Otting
4. Christoph Nitsche

Reviewed by ScreenIT

Coiled coil control of growth factor and inhibitor-dependent EGFR trafficking and degradation

1. Deepto Mozumdar
2. Sol Hsun-Hui Chang
3. Kim Quach
4. Amy Doerner
5. Alanna Schepartz

• Curated by eLife

  Evaluation Summary:

  This manuscript investigates the cellular role of the juxtamembrane region in the EGF receptor, a poorly understood portion of the EGFR cytosolic domain that connects the transmembrane segment to the kinase domain. Through a series of well-designed experiments, the work shows that the endocytic trafficking route of EGFR following its activation is determined by the juxtamembrane coiled-coil conformation in a model cell line. This finding is important for three reasons. It identifies a critical role for the juxtamembrane region; it resolves the discrepancy that TGF-beta dissociation from EGFR is supposed to occur at higher pH, yet the EGFR-TGF-beta complex continues to signal from endosomes; and it pinpoints the mechanism of EGFR inhibition by a new class of tyrosine kinase inhibitors.

  (This preprint has been reviewed by eLife. We include the public reviews from the reviewers here; the authors also receive private feedback with suggested changes to the manuscript. All reviewers agreed to share their names with the authors.)

Reviewed by eLife