Biochemistry

Disulfide bridge-dependent dimerization triggers FGF2 membrane translocation into the extracellular space

1. Fabio Lolicato
2. Julia P Steringer
3. Roberto Saleppico
4. Daniel Beyer
5. Jaime Fernandez-Sobaberas
6. Sebastian Unger
7. Steffen Klein
8. Petra Riegerová
9. Sabine Wegehingel
10. Hans-Michael Müller
11. Xiao J Schmitt
12. Shreyas Kaptan
13. Christian Freund
14. Martin Hof
15. Radek Šachl
16. Petr Chlanda
17. Ilpo Vattulainen
18. Walter Nickel

• Curated by eLife

  eLife assessment

  This manuscript reports important findings, demonstrating a critical role for a cysteine-containing dimerization interface in the secretion of FGF2 through an unconventional pathway. The authors provide compelling evidence, combining in vitro biochemical assays with structural simulation. The work will be of interest to researchers working on protein trafficking and secretion.

Reviewed by eLife

Mitochondrial temperature homeostasis resists external metabolic stresses

1. Mügen Terzioglu
2. Kristo Veeroja
3. Toni Montonen
4. Teemu O Ihalainen
5. Tiina S Salminen
6. Paule Bénit
7. Pierre Rustin
8. Young-Tae Chang
9. Takeharu Nagai
10. Howard T Jacobs

• Curated by eLife

  eLife assessment

  The study provides useful data supporting prior findings that mitochondria in cultured cells maintain a temperature that is up to 15°C above the external temperature at which cultured cells are maintained. The evidence supporting the hypothesis is solid, although direct measures of temperature in isolated mitochondria or comparison with other cellular compartments would have strengthened the ability to interpret the relevance of the findings. Nevertheless, the bioenergetic implications of the work will be of interest to cell biologists, biochemists, and physiologists.

Reviewed by eLife

Mechanism of stepwise electron transfer in six-transmembrane epithelial antigen of the prostate (STEAP) 1 and 2

1. Kehan Chen
2. Lie Wang
3. Jiemin Shen
4. Ah-Lim Tsai
5. Ming Zhou
6. Gang Wu

• Curated by eLife

  eLife assessment

  This study provides useful insights into the mechanisms of electron transport in STEAP proteins, consistent with current models. The work strengthens and supports previously published biochemical and structural data, and the experimental results are of solid technical quality. The manuscript will be of interest to colleagues who work on STEAP proteins and related electron transfer systems.

Reviewed by eLife

What AlphaFold tells us about cohesin’s retention on and release from chromosomes

1. Kim A Nasmyth
2. Byung-Gil Lee
3. Maurici Brunet Roig
4. Jan Löwe

• Curated by eLife

  eLife assessment

  This important study makes use of AlphaFold2 to predict the models of tens of cohesin subcomplexes from different species. The models, which are in most cases consistent with published cohesin variants with compromised in vitro and in vivo cohesin activity, provide convincing evidence that leads to testable hypotheses of cohesin dynamics and regulation. More broadly, this study serves as an example of how to use AlphaFold2 to build models of protein complexes that involve the docking of flexible regions to globular domains.

Reviewed by eLife

Engineered Active Zymogen of Microbial Transglutaminase

1. Ryutaro Ariyoshi
2. Takashi Matsuzaki
3. Ryo Sato
4. Kosuke Minamihata
5. Kounosuke Hayashi
6. Rie Wakabayashi
7. Masahiro Goto
8. Noriho Kamiya

Reviewed by Arcadia Science

Structural basis of EHEP-mediated offense against phlorotannin-induced defense from brown algae to protect akuBGL activity

1. Xiaomei Sun
2. Yuxin Ye
3. Naofumi Sakurai
4. Hang Wang
5. Koji Kato
6. Jian Yu
7. Keizo Yuasa
8. Akihiko Tsuji
9. Min Yao

• Curated by eLife

  eLife assessment

  This important study presents convincing evidence on how the sea slug Aplysia kurodai optimizes its digestion of brown algae, in a classical predator-prey 'arms race' at the molecular level. The experimental protein structures and enzyme assays provide support for the claims of how A. kurodai avoids inhibition by algal compounds, and also hold promise for biotechnological applications.

Reviewed by eLife

The structural basis for deubiquitination by the fingerless USP-type effector TssM

1. Thomas Hermanns
2. Matthias Uthoff
3. Ulrich Baumann
4. Kay Hofmann

Reviewed by Review Commons

Structural foundation for the role of enterococcal PrgB in conjugation, biofilm formation, and virulence

1. Wei-Sheng Sun
2. Lena Lassinantti
3. Michael Järvå
4. Andreas Schmitt
5. Josy ter Beek
6. Ronnie P-A Berntsson

• Curated by eLife

  eLife assessment

  This study presents valuable structural data for the bacterial adhesin PrgB, an atypical microbial cell surface-anchored polypeptide that binds DNA. There is convincing support for the claims regarding the overall function and importance of individual domains, which integrate a wide range of new and previously published experimental data. The structure-based model of PrgB molecular activity will be impactful in the field of bacterial adhesins, conjugation, and biofilm formation, especially because it focuses on a clinically relevant Gram-positive pathogen, whereas most work in the field has been focused on Gram-negative model systems.

Reviewed by eLife

Vibrio cholerae’s ToxRS bile sensing system

1. Nina Gubensäk
2. Theo Sagmeister
3. Christoph Buhlheller
4. Bruno Di Geronimo
5. Gabriel E Wagner
6. Lukas Petrowitsch
7. Melissa A Gräwert
8. Markus Rotzinger
9. Tamara M Ismael Berger
10. Jan Schäfer
11. Isabel Usón
12. Joachim Reidl
13. Pedro A Sánchez-Murcia
14. Klaus Zangger
15. Tea Pavkov-Keller

• Curated by eLife

  eLife assessment

  This study provides important insights into the structural biology and molecular mechanism of the sensory proteins ToxR/S that are associated with survival and virulence of the cholera pathogen. The structural studies are solid and supported by a series of biophysical experiments revealing a split, periplasmic protein binding interface for bile acid. The results are of interest to both protein biochemistry and pharmacology, potentially opening new routes for intervention in cholera disease.

Reviewed by eLife

Indole produced during dysbiosis mediates host–microorganism chemical communication

1. Rui-Qiu Yang
2. Yong-Hong Chen
3. Qin-yi Wu
4. Jie Tang
5. Shan-Zhuang Niu
6. Qiu Zhao
7. Yi-Cheng Ma
8. Cheng-Gang Zou

• Curated by eLife

  eLife assessment

  This fundamental study provides compelling evidence for a new mechanism of host-microbe interaction, with indole, produced by proliferating bacteria in the C. elegans digestive system, signalling through the host via the transcription factor DAF-16 to induce the expression of genes controlling bacterial growth in the gut. The work is relevant to a wide audience as it invites deeper research into this mechanism, while also serving as a template for similar microbiome/host interactions in other systems.

Reviewed by eLife