A single-cell atlas of spatial and temporal gene expression in the mouse cranial neural plate
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eLife Assessment
This study reports an important new scRNAseq atlas of the mouse cranial neural plate during neural induction, patterning, and morphogenesis. The study includes a robust analysis of scRNAseq datasets covering six distinct developmental stages, as well as data describing the global transcriptional response of neural plate cells to a key ventralizing signaling molecule, Sonic Hedgehog. The computational data and validation of gene expression patterns are convincing, making this a helpful resource for investigators studying the early development of the cranial neural plate and cranial mesoderm.
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Abstract
The formation of the mammalian brain requires regionalization and morphogenesis of the cranial neural plate, which transforms from an epithelial sheet into a closed tube that provides the structural foundation for neural patterning and circuit formation. Sonic hedgehog (SHH) signaling is important for cranial neural plate patterning and closure, but the transcriptional changes that give rise to the spatially regulated cell fates and behaviors that build the cranial neural tube have not been systematically analyzed. Here we used single-cell RNA sequencing to generate an atlas of gene expression at six consecutive stages of cranial neural tube closure in the mouse embryo. Ordering transcriptional profiles relative to the major axes of gene expression predicted spatially regulated expression of 870 genes along the anterior-posterior and mediolateral axes of the cranial neural plate and reproduced known expression patterns with over 85% accuracy. Single-cell RNA sequencing of embryos with activated SHH signaling revealed distinct SHH-regulated transcriptional programs in the developing forebrain, midbrain, and hindbrain, suggesting a complex interplay between anterior-posterior and mediolateral patterning systems. These results define a spatiotemporally resolved map of gene expression during cranial neural tube closure and provide a resource for investigating the transcriptional events that drive early mammalian brain development.
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eLife Assessment
This study reports an important new scRNAseq atlas of the mouse cranial neural plate during neural induction, patterning, and morphogenesis. The study includes a robust analysis of scRNAseq datasets covering six distinct developmental stages, as well as data describing the global transcriptional response of neural plate cells to a key ventralizing signaling molecule, Sonic Hedgehog. The computational data and validation of gene expression patterns are convincing, making this a helpful resource for investigators studying the early development of the cranial neural plate and cranial mesoderm.
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Reviewer #1 (Public review):
Summary:
This impressive study presents a comprehensive scRNAseq atlas of the cranial region during neural induction, patterning, and morphogenesis. The authors collected a robust scRNAseq dataset covering six distinct developmental stages. The analysis focused on the neural tissue, resulting in a highly detailed temporal map of neural plate development. The findings demonstrate how different cell fates are organized in specific spatial patterns along the anterior-posterior and medial-lateral axes within the developing neural tissue. Additionally, the research utilized high-density single-cell RNA sequencing (scRNAseq) to reveal intricate spatial and temporal patterns independent of traditional spatial techniques.
The investigation utilized diffusion component analysis to spatially order cells based on their …
Reviewer #1 (Public review):
Summary:
This impressive study presents a comprehensive scRNAseq atlas of the cranial region during neural induction, patterning, and morphogenesis. The authors collected a robust scRNAseq dataset covering six distinct developmental stages. The analysis focused on the neural tissue, resulting in a highly detailed temporal map of neural plate development. The findings demonstrate how different cell fates are organized in specific spatial patterns along the anterior-posterior and medial-lateral axes within the developing neural tissue. Additionally, the research utilized high-density single-cell RNA sequencing (scRNAseq) to reveal intricate spatial and temporal patterns independent of traditional spatial techniques.
The investigation utilized diffusion component analysis to spatially order cells based on their positioning along the anterior-posterior axis, corresponding to the forebrain, midbrain, hindbrain, and medial-lateral axis. By cross-referencing with MGI expression data, the identification of cell types was validated, affirming the expression patterns of numerous known genes and implicating others as differentially expressed along these axes. These findings significantly advance our understanding of the spatially regulated genes in neural tissues during early developmental stages. The emphasis on transcription factors, cell surface, and secreted proteins provides valuable insights into the intricate gene regulatory networks underpinning neural tissue patterning. Analysis of a second scRNAseq dataset where Shh signaling was inhibited by culturing embryos in SAG identified known and previously unknown transcripts regulated by Shh, including the Wnt pathway.
The data includes the neural plate and captures all major cell types in the head, including the mesoderm, endoderm, non-neural ectoderm, neural crest, notochord, and blood. With further analyses, this high-quality data promises to significantly advance our understanding of how these tissues develop in conjunction with the neural tissue, paving the way for future breakthroughs in developmental biology and genomics.
Strengths:
The data is well presented in the figures and thoroughly described in the text. The quality of the scRNAseq data and bioinformatic analysis is exceptional.
Weaknesses:
No weaknesses were identified by this reviewer.
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Reviewer #2 (Public review):
Summary:
Brooks et al. generate a gene expression atlas of the early embryonic cranial neural plate. They generate single-cell transcriptome data from early cranial neural plate cells at 6 consecutive stages between E7.5 to E9. Utilizing computational analysis they infer temporal gene expression dynamics and spatial gene expression patterns along the anterior-posterior and mediolateral axis of the neural plate. Subsequent comparison with known gene expression patterns revealed a good agreement with their inferred patterns, thus validating their approach. They then focus on Sonic Hedgehog (Shh) signalling, a key morphogen signal, whose activities partition the neural plate into distinct gene expression domains along the mediolateral axis. Single-cell transcriptome analysis of embryos in which the Shh pathway …
Reviewer #2 (Public review):
Summary:
Brooks et al. generate a gene expression atlas of the early embryonic cranial neural plate. They generate single-cell transcriptome data from early cranial neural plate cells at 6 consecutive stages between E7.5 to E9. Utilizing computational analysis they infer temporal gene expression dynamics and spatial gene expression patterns along the anterior-posterior and mediolateral axis of the neural plate. Subsequent comparison with known gene expression patterns revealed a good agreement with their inferred patterns, thus validating their approach. They then focus on Sonic Hedgehog (Shh) signalling, a key morphogen signal, whose activities partition the neural plate into distinct gene expression domains along the mediolateral axis. Single-cell transcriptome analysis of embryos in which the Shh pathway was pharmacologically activated throughout the neural plate revealed characteristic changes in gene expression along the mediolateral axis and the induction of distinct Shh-regulated gene expression programs in the developing fore-, mid-, and hindbrain.
Strengths:
This manuscript provides a comprehensive transcriptomic characterisation of the developing cranial neural plate, a part of the embryo that to my knowledge has not been extensively analysed by single-cell transcriptomic approaches. The single-cell sequencing data appears to be of high quality and will be a great resource for the wider scientific community. Moreover, the computational analysis is well executed and the validation of the sequencing data using published gene expression patterns is convincing. Taken together, this is a well-executed study that describes a relevant scientific resource for the wider scientific community.
Weaknesses:
Conceptually, the findings that gene expression patterns differ along the rostrocaudal, mediolateral, and temporal axes of the neural plate and that Shh signalling induces distinct target genes along the anterior-posterior axis of the nervous system are more expected than surprising. However, the strength of this manuscript is again the comprehensive characterization of the spatiotemporal gene expression patterns and how they change upon ectopic activation of the Shh pathway.
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Reviewer #3 (Public review):
Summary:
The authors performed a detailed single-cell analysis of the early embryonic cranial neural plate with unprecedented temporal resolution between embryonic days 7.5 and 8.75. They employed diffusion analysis to identify genes that correspond to different temporal and spatial locations within the embryo. Finally, they also examined the global response of cranial tissue to a Smoothened agonist.
Strengths:
Overall, this is an impressive resource, well-validated against sets of genes with known temporal and spatial patterns of expression. It will be of great value to investigators examining the early stages of neural plate patterning, neural progenitor diversity, and the roles of signaling molecules and gene regulatory networks controlling the regionalization and diversification of the neural plate.
Weakn…
Reviewer #3 (Public review):
Summary:
The authors performed a detailed single-cell analysis of the early embryonic cranial neural plate with unprecedented temporal resolution between embryonic days 7.5 and 8.75. They employed diffusion analysis to identify genes that correspond to different temporal and spatial locations within the embryo. Finally, they also examined the global response of cranial tissue to a Smoothened agonist.
Strengths:
Overall, this is an impressive resource, well-validated against sets of genes with known temporal and spatial patterns of expression. It will be of great value to investigators examining the early stages of neural plate patterning, neural progenitor diversity, and the roles of signaling molecules and gene regulatory networks controlling the regionalization and diversification of the neural plate.
Weaknesses:
The manuscript should be considered a resource. Experimental manipulation is limited to the analysis of neural plate cells that were cultured in vitro for 12 hours with SAG. Besides the identification of a significant set of previously unreported genes that are differentially expressed in the cranial neural plate, there is little new biological insight emerging from this study. Some additional analyses might help to highlight novel hypotheses arising from this remarkable resource.
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