A chromosome-scale reference genome of Pteropus pselaphon
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The Bonin flying fox ( Pteropus pselaphon ) is an endemic species of the Ogasawara Islands, Japan, and is classified as a Critically Endangered species. Here, we report a chromosome- scale genome assembly of P. pselaphon , constructed using Nanopore long reads and Omni-C scaffolding. The final assembly spans 2.16 Gb across 929 scaffolds and achieves a Benchmarking Universal Single-Copy Orthologs (BUSCO) completeness score of C:98.2% [S:94.3%, D:4.0%], F:0.7%, M:1.0%. Gene prediction with Helixer software identified 20,647 protein-coding sequences, with a BUSCO score of C:92.2% [S:88.9%, D:3.3%], F:3.3%, M:4.5%. This genome provides a valuable resource for the conservation management of this endangered species, as well as for evolutionary genomics and studies of host–pathogen interactions in flying fox.