Annotated Sequence Record - Complete genomic sequences of two novel Ralstonia jumbo phages isolated from leaf litter compost

Two Ralstonia phages, FLC1-1B and FLC4-3B, were isolated from leaf litter compost, using Ralstonia pseudosolanacearum , which is a causal agent of bacterial wilt disease, as a host. The genomic DNA sequences of FLC1-1B and FLC4-3B were 290,008 bp and 291,257 bp, respectively, and they were therefore classified as jumbo phages. However, they did not show high similarity to any jumbo phage genomic sequences according to a blastn search using the NCBI nt database. The closest hit was to the jumbo phage Ripduovirus RP12 with only 35% coverage and 77% sequence identity, whereas 99.0% of the genomic sequence between FLC1-1B and FLC4-3B was similar. Based on these findings, FLC1-1B and FLC4-3B should be classified into a different genus of the order Caudoviricetes . The wilt disease–suppressive activity of FLC4-3B in tomato plants suggested that these two Ralstonia phages have potential application as a biocontrol agent for managing R . pseudosolanacearum .