The Complete Mitochondrial Genome of a Newly Recorded Chinese Species of Diglyphus sabulosus (Hymenoptera: Eulophidae) and Insights into Its Phylogenetic Position

Diglyphus Walker, 1844 is an economically important genus which many species acting as biocontrol agents against agromyzid leafminer pests, but there is a lack of mitogenomic data on the evolutionary relationships within this genus, hindering a comprehensive understanding of its evolutionary history. we used traditional morphological methods to identify species, and present the first complete mitochondrial genome sequence and characterization of features of Diglyphus sabulosus and further infer its phylogenetic position based on the complete mitochondrial genome sequence. The complete mitochondrial genome of Diglyphus sabulosus is 15,690 bp in length, including 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and 1 control region. The AT content of the whole genome sequence was 81.0%, indicating a significant AT bias. All 13 protein-coding genes have the typical ATN as the start codon and TAA as the stop codon. Phylogenetic analysis inferred from the complete mitogenome revealed that all species within the family Eulophidae constituted a monophyletic clade, supporting the monophyly of this family. Diglyphus sabulosus and Diglyphus poppoea form a well-supported sister group, representing the species with the closest phylogenetic relationship within the analyzed taxa. This inferred close phylogenetic affinity is highly consistent with their morphological characteristics, which exhibit the highest degree of similarity among the studied species. In this study, the mitogenome structure was analyzed and the taxonomic status of Diglyphus sabulosus was clarified, thus providing a theoretical basis for understanding the phylogenetic relationships of Diglyphus .